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Protein

Obscurin-like protein 1

Gene

Obsl1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Core component of the 3M complex, a complex required to regulate microtubule dynamics and genome integrity. It is unclear how the 3M complex regulates microtubules, it could act by controlling the level of a microtubule stabilizer. Acts as a regulator of the Cul7-RING(FBXW8) ubiquitin-protein ligase, playing a critical role in the ubiquitin ligase pathway that regulates Golgi morphogenesis and dendrite patterning in brain. Required to localize CUL7 to the Golgi apparatus in neurons (By similarity).By similarity

GO - Molecular functioni

  • actin filament binding Source: GO_Central
  • muscle alpha-actinin binding Source: GO_Central
  • structural constituent of muscle Source: GO_Central

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-MMU-8951664 Neddylation

Names & Taxonomyi

Protein namesi
Recommended name:
Obscurin-like protein 1
Gene namesi
Name:Obsl1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2138628 Obsl1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004302501 – 1804Obscurin-like protein 1Add BLAST1804

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei10PhosphoserineBy similarity1
Disulfide bondi33 ↔ 84PROSITE-ProRule annotation
Disulfide bondi149 ↔ 209PROSITE-ProRule annotation
Disulfide bondi267 ↔ 319PROSITE-ProRule annotation
Disulfide bondi362 ↔ 412PROSITE-ProRule annotation
Disulfide bondi738 ↔ 788PROSITE-ProRule annotation
Disulfide bondi829 ↔ 879PROSITE-ProRule annotation
Disulfide bondi920 ↔ 970PROSITE-ProRule annotation
Disulfide bondi1011 ↔ 1061PROSITE-ProRule annotation
Disulfide bondi1103 ↔ 1153PROSITE-ProRule annotation
Disulfide bondi1195 ↔ 1245PROSITE-ProRule annotation
Disulfide bondi1289 ↔ 1430PROSITE-ProRule annotation
Disulfide bondi1558 ↔ 1608PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

MaxQBiD3YYU8
PaxDbiD3YYU8
PeptideAtlasiD3YYU8
PRIDEiD3YYU8

PTM databases

iPTMnetiD3YYU8
PhosphoSitePlusiD3YYU8

Expressioni

Gene expression databases

BgeeiENSMUSG00000026211 Expressed in 183 organ(s), highest expression level in internal carotid artery
ExpressionAtlasiD3YYU8 baseline and differential
GenevisibleiD3YYU8 MM

Interactioni

Subunit structurei

Component of the 3M complex, composed of core components CUL7, CCDC8 and OBSL1. Interacts with CCDC8. Interacts with CUL7; the interaction is direct. Interacts with FBXW8. Interacts (via N-terminal Ig-like domain) with TTN/titin (via C-terminal Ig-like domain); the interaction is direct (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000109197

Structurei

3D structure databases

ProteinModelPortaliD3YYU8
SMRiD3YYU8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 100Ig-like 1Add BLAST89
Domaini128 – 225Ig-like 2Add BLAST98
Domaini241 – 330Ig-like 3Add BLAST90
Domaini339 – 425Ig-like 4Add BLAST87
Domaini517 – 615Fibronectin type-IIIPROSITE-ProRule annotationAdd BLAST99
Domaini720 – 800Ig-like 5Add BLAST81
Domaini804 – 891Ig-like 6Add BLAST88
Domaini902 – 982Ig-like 7Add BLAST81
Domaini986 – 1075Ig-like 8Add BLAST90
Domaini1078 – 1165Ig-like 9Add BLAST88
Domaini1176 – 1261Ig-like 10Add BLAST86
Domaini1266 – 1442Ig-like 11Add BLAST177
Domaini1536 – 1621Ig-like 12Add BLAST86
Domaini1625 – 1694Ig-like 13Add BLAST70
Domaini1702 – 1798Ig-like 14Add BLAST97

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni17 – 19Interaction with TTNBy similarity3
Regioni85 – 94Interaction with TTNBy similarity10

Keywords - Domaini

Immunoglobulin domain, Repeat

Phylogenomic databases

eggNOGiKOG0613 Eukaryota
ENOG410XQFD LUCA
GeneTreeiENSGT00860000133679
HOGENOMiHOG000263472
HOVERGENiHBG067176
InParanoidiD3YYU8
KOiK19574
OMAiRYRMEQK
OrthoDBiEOG091G0003
PhylomeDBiD3YYU8

Family and domain databases

CDDicd00063 FN3, 1 hit
Gene3Di2.60.40.10, 19 hits
InterProiView protein in InterPro
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR013098 Ig_I-set
IPR003599 Ig_sub
IPR003598 Ig_sub2
PfamiView protein in Pfam
PF07679 I-set, 13 hits
SMARTiView protein in SMART
SM00409 IG, 17 hits
SM00408 IGc2, 14 hits
SUPFAMiSSF48726 SSF48726, 17 hits
SSF49265 SSF49265, 1 hit
PROSITEiView protein in PROSITE
PS50853 FN3, 1 hit
PS50835 IG_LIKE, 14 hits

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: D3YYU8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MKAGSGDQGS PPCFLRFPRP VRVVSGAEAE LKCVVLGEPP PTVVWEKGGQ
60 70 80 90 100
QLVASERLSF PEDGAEHGLL LSGALPTDAG VYVCRARNAA GEAYAAAAVT
110 120 130 140 150
VLEPPAPEPE PESSECPLPT PGTGEGAPKF LTGPQSQWVL RGEEVVLTCQ
160 170 180 190 200
VGGLPEPKLY WEKDGMALDE VWDSSHFKLE PGRGASDEGA SLTLRILAAR
210 220 230 240 250
LPDSGVYVCH ARNAHGHAQA GALLQVHQPR ESPPQDPDEN PKPVLEPLKG
260 270 280 290 300
APKTFWVNEG KHAKFRCYVM GKPEPEIEWH LEGRPLLPDR RRLMYRDRDG
310 320 330 340 350
GFVLKVLYCQ AKDRGLYVCA ARNSAGQTLS AVQLHVKEPR LRFTRPLQDV
360 370 380 390 400
EGREHGIVVL ECKVPNSRIP TAWFREDQRL LPCRKYEQIE EGAVRRLVIH
410 420 430 440 450
KLKADDDGVY LCEMRGRVRT VANVTVKGPI LKRLPRKLDV LEGENAVLLV
460 470 480 490 500
ETQEAGVQGC WSRDGEDLPD TCQSSCGHMH ALVLPGVTRE DAGEITFSLG
510 520 530 540 550
NSRTTTLLRV KCVKHSPPGP PVMVEMFKGQ KNKVLLTWKP PEPPPETSFI
560 570 580 590 600
YRLERQEVGS DDWIQCFSIE KAGAVEVPGD CVPTEGDYHF RICTVSEHGR
610 620 630 640 650
SPHVVFNGSA HLVPTARLVS GLEDVQVYDG EDAVFSLDLS AIIQGSWFLN
660 670 680 690 700
GEQLQSNEPE GQVEPGALRY RIEQKGLQHR LILQAVKHRD SGALVGFSCP
710 720 730 740 750
GVQDSAALTI QESSVHILSP QDKVSLTFTT SERVVLTCEL SRVDFPATWY
760 770 780 790 800
KDGQKVEESE SLIVKTEGRK HRLILPEAQV RDSGEFECRT EGVSAFFGVT
810 820 830 840 850
VQDPPVHIVN PQEHVFVHAI TSECVRLTCE VDREDTTVHW YKDGQEVEES
860 870 880 890 900
DIIVLENKGP HHRLVLPAAR PSDGGEFQCV AGDERAYFTV TITDVFSWIV
910 920 930 940 950
YPSSEVHVAA VRLERVVLTC ELCRPWAEVR WTKDGEEVVE SPALLLEKED
960 970 980 990 1000
TIRRLVLPSV QLEDSGEYLC EIHDESASFT ITVTEPPVRI IYPQDEVTLH
1010 1020 1030 1040 1050
AVSLECVVLT CELSREDAPV RWYKDGLEVE ESEALVLQSD GPRRRLVLPA
1060 1070 1080 1090 1100
AQPEDGGEFV CDAGDDSAFF TVTVTAPPER IVHPAARSLD LQFGAPGHVE
1110 1120 1130 1140 1150
LRCEVAPAGS QVRWYKDGLE VEVSDALQLG AEGPARTLTL PHAQPEDAGE
1160 1170 1180 1190 1200
YVCETRDEAV TFNVSLAELP VQFLAPEAAP NPLCVVPGEP VVLSCELSRA
1210 1220 1230 1240 1250
SAQVFWSHNG SPVQQGEGLE LRAEGPRRIL CIQAADLAHT GVYTCQSGAS
1260 1270 1280 1290 1300
PGAPSLSFNV QVAELPPVKL VSELTPLTVH EGDDATFQCE VSPPDAEVTW
1310 1320 1330 1340 1350
LRNGAVITAG PQLEMVQNGS SRTLIIRGCQ LKDAGTVTAR AGAADTSARL
1360 1370 1380 1390 1400
HVRETELLFL RRLQDVRAEE GQDVHLEVET GRVGAAGTVR WIRGGEPLPL
1410 1420 1430 1440 1450
DSRLTTAQDG HVHRLSIHGV LLTDQGTYGC ESRHDRTLAR LSVRPRQLRE
1460 1470 1480 1490 1500
LRPLEDVTVH EGGSATFQLE LSQEGVTGEW AQGGVRLHPG PKCHIQSEGR
1510 1520 1530 1540 1550
THRLVLSGLG LADSGCVSFT ADTLRCAARL TVREVPVTIV QGPQDLEVTE
1560 1570 1580 1590 1600
GDTATFECEL SQTLADVIWE KDGQALSLSP RLRLQALGTR RLLLLRRCCS
1610 1620 1630 1640 1650
SDAGTYSCVV GTARSEPARL TVREREVSVL RELRSVSARE GDGATFECTV
1660 1670 1680 1690 1700
SETEITGRWE LGGRALRPGG RVRIRQEGKK HILVLSELRT EDTGEVCFQA
1710 1720 1730 1740 1750
GPAQSLARLE VEALPLQMCR RPPREKTVLV NRRAVLEVTV SRPGGHVCWM
1760 1770 1780 1790 1800
REGVELCPGN KYETRRHGTT HSLVIHDVRP EDQGTYSCQA GQDSADTQLL

VDGD
Length:1,804
Mass (Da):197,937
Last modified:April 20, 2010 - v1
Checksum:iFDD14572E8FC10B2
GO
Isoform 2 (identifier: D3YYU8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-478: Missing.
     985-1004: EPPVRIIYPQDEVTLHAVSL → GVGLSQPPESPEDNPEPQEC
     1005-1804: Missing.

Show »
Length:526
Mass (Da):58,494
Checksum:iE9F05F4CEB458A4E
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0B4J1L4A0A0B4J1L4_MOUSE
Obscurin-like protein 1
Obsl1
1,025Annotation score:
A0A0A0MQG1A0A0A0MQG1_MOUSE
Obscurin-like protein 1
Obsl1
1,162Annotation score:
F7AGE9F7AGE9_MOUSE
Obscurin-like protein 1
Obsl1
417Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti786F → I in AAH51643 (PubMed:15489334).Curated1
Sequence conflicti858K → E in AAH51643 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0559051 – 478Missing in isoform 2. 1 PublicationAdd BLAST478
Alternative sequenceiVSP_055906985 – 1004EPPVR…HAVSL → GVGLSQPPESPEDNPEPQEC in isoform 2. 1 PublicationAdd BLAST20
Alternative sequenceiVSP_0559071005 – 1804Missing in isoform 2. 1 PublicationAdd BLAST800

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC115011 Genomic DNA No translation available.
BC051643 mRNA Translation: AAH51643.1
CCDSiCCDS15077.2 [D3YYU8-1]
RefSeqiNP_849215.3, NM_178884.5 [D3YYU8-1]
UniGeneiMm.236454

Genome annotation databases

EnsembliENSMUST00000113567; ENSMUSP00000109197; ENSMUSG00000026211 [D3YYU8-1]
GeneIDi98733
KEGGimmu:98733
UCSCiuc007bpn.2 mouse [D3YYU8-1]
uc007bpp.3 mouse [D3YYU8-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC115011 Genomic DNA No translation available.
BC051643 mRNA Translation: AAH51643.1
CCDSiCCDS15077.2 [D3YYU8-1]
RefSeqiNP_849215.3, NM_178884.5 [D3YYU8-1]
UniGeneiMm.236454

3D structure databases

ProteinModelPortaliD3YYU8
SMRiD3YYU8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000109197

PTM databases

iPTMnetiD3YYU8
PhosphoSitePlusiD3YYU8

Proteomic databases

MaxQBiD3YYU8
PaxDbiD3YYU8
PeptideAtlasiD3YYU8
PRIDEiD3YYU8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000113567; ENSMUSP00000109197; ENSMUSG00000026211 [D3YYU8-1]
GeneIDi98733
KEGGimmu:98733
UCSCiuc007bpn.2 mouse [D3YYU8-1]
uc007bpp.3 mouse [D3YYU8-2]

Organism-specific databases

CTDi23363
MGIiMGI:2138628 Obsl1

Phylogenomic databases

eggNOGiKOG0613 Eukaryota
ENOG410XQFD LUCA
GeneTreeiENSGT00860000133679
HOGENOMiHOG000263472
HOVERGENiHBG067176
InParanoidiD3YYU8
KOiK19574
OMAiRYRMEQK
OrthoDBiEOG091G0003
PhylomeDBiD3YYU8

Enzyme and pathway databases

ReactomeiR-MMU-8951664 Neddylation

Miscellaneous databases

ChiTaRSiObsl1 mouse
PROiPR:D3YYU8
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026211 Expressed in 183 organ(s), highest expression level in internal carotid artery
ExpressionAtlasiD3YYU8 baseline and differential
GenevisibleiD3YYU8 MM

Family and domain databases

CDDicd00063 FN3, 1 hit
Gene3Di2.60.40.10, 19 hits
InterProiView protein in InterPro
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR013098 Ig_I-set
IPR003599 Ig_sub
IPR003598 Ig_sub2
PfamiView protein in Pfam
PF07679 I-set, 13 hits
SMARTiView protein in SMART
SM00409 IG, 17 hits
SM00408 IGc2, 14 hits
SUPFAMiSSF48726 SSF48726, 17 hits
SSF49265 SSF49265, 1 hit
PROSITEiView protein in PROSITE
PS50853 FN3, 1 hit
PS50835 IG_LIKE, 14 hits
ProtoNetiSearch...

Entry informationi

Entry nameiOBSL1_MOUSE
AccessioniPrimary (citable) accession number: D3YYU8
Secondary accession number(s): F7AD47, Q80WA6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 3, 2014
Last sequence update: April 20, 2010
Last modified: November 7, 2018
This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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