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Protein

Scaffold attachment factor B1

Gene

Safb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing. Can function as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. Can inhibit cell proliferation. When associated with RBMX, binds to and stimulates transcription from the SREBF1 promoter.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Repressor, RNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-3899300 SUMOylation of transcription cofactors

Names & Taxonomyi

Protein namesi
Recommended name:
Scaffold attachment factor B1
Short name:
SAF-B1
Gene namesi
Name:Safb
Synonyms:Safb1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:2146974 Safb

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00004130222 – 937Scaffold attachment factor B1Add BLAST936

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei24PhosphoserineBy similarity1
Modified residuei55PhosphoserineBy similarity1
Modified residuei79PhosphoserineBy similarity1
Cross-linki172Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki186Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei188PhosphothreonineBy similarity1
Modified residuei195PhosphoserineBy similarity1
Modified residuei197PhosphoserineBy similarity1
Modified residuei209PhosphoserineBy similarity1
Cross-linki231Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Cross-linki316Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Cross-linki403Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei405PhosphoserineBy similarity1
Modified residuei406PhosphoserineBy similarity1
Cross-linki414Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei437PhosphoserineBy similarity1
Cross-linki505Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki536Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki565Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki592Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki600Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linki600Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei602PhosphoserineBy similarity1
Modified residuei604PhosphoserineBy similarity1
Modified residuei623PhosphoserineBy similarity1
Modified residuei626PhosphoserineCombined sources1
Modified residuei629N6-acetyllysineBy similarity1
Modified residuei832Omega-N-methylarginineCombined sources1
Cross-linki869Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei890Asymmetric dimethylarginineCombined sources1
Modified residuei896Asymmetric dimethylarginineCombined sources1
Modified residuei906Asymmetric dimethylarginineCombined sources1
Modified residuei912Asymmetric dimethylarginineCombined sources1

Post-translational modificationi

Sumoylated by PIAS1 with SUMO1 and SUMO2/3, desumoylated by SENP1. Sumoylation is required for transcriptional repressor activity (By similarity).By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiD3YXK2
MaxQBiD3YXK2
PaxDbiD3YXK2
PeptideAtlasiD3YXK2
PRIDEiD3YXK2

PTM databases

iPTMnetiD3YXK2
PhosphoSitePlusiD3YXK2

Expressioni

Gene expression databases

BgeeiENSMUSG00000071054 Expressed in 295 organ(s), highest expression level in cerebellum
ExpressionAtlasiD3YXK2 baseline and differential
GenevisibleiD3YXK2 MM

Interactioni

Subunit structurei

Monomer. Can form homodimers. Interacts with KHDRBS3, POLR2A, SAFB2 or SFRS1, SFRS9 and TRA2B/SFRS10 (By similarity). Interacts with RBMX. Interacts with SRPK1 and inhibits its activity (By similarity).By similarity

Protein-protein interaction databases

BioGridi230338, 5 interactors
IntActiD3YXK2, 4 interactors
STRINGi10090.ENSMUSP00000092849

Structurei

3D structure databases

ProteinModelPortaliD3YXK2
SMRiD3YXK2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 65SAPPROSITE-ProRule annotationAdd BLAST35
Domaini428 – 506RRMPROSITE-ProRule annotationAdd BLAST79

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni550 – 814Interaction with POLR2ABy similarityAdd BLAST265
Regioni621 – 937Interaction with SAFB2By similarityAdd BLAST317

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili652 – 726Sequence analysisAdd BLAST75

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi621 – 638Nuclear localization signalSequence analysisAdd BLAST18

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi96 – 285Glu-richAdd BLAST190
Compositional biasi634 – 853Arg-richAdd BLAST220
Compositional biasi640 – 726Glu-richAdd BLAST87
Compositional biasi807 – 925Gly-richAdd BLAST119

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4661 Eukaryota
ENOG4111F1G LUCA
GeneTreeiENSGT00730000110777
HOGENOMiHOG000092533
InParanoidiD3YXK2
PhylomeDBiD3YXK2
TreeFamiTF325240

Family and domain databases

Gene3Di1.10.720.30, 1 hit
3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
IPR034781 SAFB1
IPR003034 SAP_dom
IPR036361 SAP_dom_sf
PANTHERiPTHR15683:SF6 PTHR15683:SF6, 1 hit
PfamiView protein in Pfam
PF00076 RRM_1, 1 hit
PF02037 SAP, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SM00513 SAP, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
SSF68906 SSF68906, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit
PS50800 SAP, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

D3YXK2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAETLSGLGD ASAAGAAAVS SAASETGTRR LSDLRVIDLR AELKKRNLDS
60 70 80 90 100
SGNKSVLMER LKKAIEDEGG NPDEIEVTSE CNKKMPKRPS KGRKPEDEGV
110 120 130 140 150
EDNGLEENSG DGQEDVETSL ENLQDMDMMD ISVLDEADID NGSVADCVEE
160 170 180 190 200
EEEATLPEGL ADSTELVEGD LKGLPEQLQE HAIDDKDTVN NVDTSSSDFT
210 220 230 240 250
MLQEMEEASL EPENEKILDI LGETCKSEPV KEEGSELEQP FAQATSSVGP
260 270 280 290 300
DRKLAEEEDL FESCGHPEEE EEEEEEDQEE EQEEEGDLAL ASSSKSESPS
310 320 330 340 350
TRCQWSEADA PLAVVKREPA DAPGGGTGMD REPVGLEEPV EQSSTAAQLP
360 370 380 390 400
EATSQELVRA PTAALSPEPQ DSKEDVKKFA FDACNDVPAP PKESSASEGA
410 420 430 440 450
DQKMSSVEED SDTKRLSREE KGRSSCGRNF WVSGLSSTTR ATDLKNLFSR
460 470 480 490 500
YGKVVGAKVV TNARSPGARC YGFVTMSTAE EATKCISHLH KTELHGKMIS
510 520 530 540 550
VEKAKSEPTG KRVPDRRDGD SKKEKASTSD RSANLKREEK GERKDDAKKT
560 570 580 590 600
DDGSTEKSKD ADDQKPGPSE RSRTTKSGSR GTERTVVMDK SKGVPVISVK
610 620 630 640 650
TSGSKERASK SQDRKSASRE KRSVVSFDKV KESRKSRDSE SRRERERSER
660 670 680 690 700
EQRLQAQWER EERERLEIAR ERLAFHRHRL ERERMERERL ERERMHVEQE
710 720 730 740 750
RRREQERIHR EREELRRQQE LRYEQERRPA VRRPYEVDGR RDDAYWPEAK
760 770 780 790 800
RAALDDRYHS DFSRQDRFHD FDHRDRGRYP NHSVDRREGS RSMMGDREGQ
810 820 830 840 850
HYPERHGGPE RHGRDSRDGW GYGSNKRLSE GRGLPPPPRR DWGEHGRRLE
860 870 880 890 900
DDRAWQGTAD GGMMERDHKR WQGGERSMSG HSGPGHMMNR GGMSGRGSFA
910 920 930
PGGASRGHVI PRGGMQAGFG GQSRGSRPSD ARFTRRY
Length:937
Mass (Da):105,104
Last modified:April 5, 2011 - v2
Checksum:iD246A419FA465C3D
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
S4R1M2S4R1M2_MOUSE
Scaffold attachment factor B1
Safb
939Annotation score:
S4R2Q5S4R2Q5_MOUSE
Scaffold attachment factor B1
Safb
44Annotation score:
S4R1W0S4R1W0_MOUSE
Scaffold attachment factor B1
Safb
169Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CT485788 Genomic DNA No translation available.
CH466537 Genomic DNA Translation: EDL38175.1
BC018200 mRNA No translation available.
AK087504 mRNA No translation available.
CCDSiCCDS50152.1
RefSeqiNP_001156772.1, NM_001163300.1
UniGeneiMm.255066

Genome annotation databases

EnsembliENSMUST00000095224; ENSMUSP00000092849; ENSMUSG00000071054
GeneIDi224903
KEGGimmu:224903
UCSCiuc012avv.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CT485788 Genomic DNA No translation available.
CH466537 Genomic DNA Translation: EDL38175.1
BC018200 mRNA No translation available.
AK087504 mRNA No translation available.
CCDSiCCDS50152.1
RefSeqiNP_001156772.1, NM_001163300.1
UniGeneiMm.255066

3D structure databases

ProteinModelPortaliD3YXK2
SMRiD3YXK2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230338, 5 interactors
IntActiD3YXK2, 4 interactors
STRINGi10090.ENSMUSP00000092849

PTM databases

iPTMnetiD3YXK2
PhosphoSitePlusiD3YXK2

Proteomic databases

EPDiD3YXK2
MaxQBiD3YXK2
PaxDbiD3YXK2
PeptideAtlasiD3YXK2
PRIDEiD3YXK2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000095224; ENSMUSP00000092849; ENSMUSG00000071054
GeneIDi224903
KEGGimmu:224903
UCSCiuc012avv.1 mouse

Organism-specific databases

CTDi6294
MGIiMGI:2146974 Safb

Phylogenomic databases

eggNOGiKOG4661 Eukaryota
ENOG4111F1G LUCA
GeneTreeiENSGT00730000110777
HOGENOMiHOG000092533
InParanoidiD3YXK2
PhylomeDBiD3YXK2
TreeFamiTF325240

Enzyme and pathway databases

ReactomeiR-MMU-3899300 SUMOylation of transcription cofactors

Miscellaneous databases

ChiTaRSiSafb mouse
PROiPR:D3YXK2
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000071054 Expressed in 295 organ(s), highest expression level in cerebellum
ExpressionAtlasiD3YXK2 baseline and differential
GenevisibleiD3YXK2 MM

Family and domain databases

Gene3Di1.10.720.30, 1 hit
3.30.70.330, 1 hit
InterProiView protein in InterPro
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
IPR034781 SAFB1
IPR003034 SAP_dom
IPR036361 SAP_dom_sf
PANTHERiPTHR15683:SF6 PTHR15683:SF6, 1 hit
PfamiView protein in Pfam
PF00076 RRM_1, 1 hit
PF02037 SAP, 1 hit
SMARTiView protein in SMART
SM00360 RRM, 1 hit
SM00513 SAP, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
SSF68906 SSF68906, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 1 hit
PS50800 SAP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSAFB1_MOUSE
AccessioniPrimary (citable) accession number: D3YXK2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: April 5, 2011
Last modified: November 7, 2018
This is version 79 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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