UniProtKB - D3U717 (AROD3_PETHY)
Protein
Arogenate dehydratase 3
Gene
ADT3
Organism
Petunia hybrida (Petunia)
Status
Functioni
Converts the prephenate and L-arogenate produced from the shikimate-chorismate pathway into 3-phenylpyruvate and phenylalanine (Phe), respectively (PubMed:20215586). Involved in floral volatile benzenoids and phenylpropanoids (FVBP) production (By similarity).By similarity1 Publication
Catalytic activityi
- EC:4.2.1.51PROSITE-ProRule annotation1 PublicationThis reaction proceeds in the forward1 Publication direction.
- EC:4.2.1.911 PublicationThis reaction proceeds in the forward1 Publication direction.
Kineticsi
kcat is 0.194 sec(-1) with L-arogenate as substrate (PubMed:20215586). kcat is 0.012 sec(-1) with prephenate as substrate (PubMed:20215586).1 Publication
- KM=48.8 µM for L-arogenate1 Publication
- KM=465.8 µM for prephenate1 Publication
- Vmax=4.464 pmol/sec/mg enzyme with L-arogenate as substrate1 Publication
- Vmax=280 pmol/sec/mg enzyme with prephenate as substrate1 Publication
: L-phenylalanine biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes L-phenylalanine from L-arogenate.1 PublicationProteins known to be involved in this subpathway in this organism are:
- Arogenate dehydratase 3 (ADT3), Arogenate dehydratase 2 (ADT2), Arogenate dehydratase 1 (ADT1)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-phenylalanine from L-arogenate, the pathway L-phenylalanine biosynthesis and in Amino-acid biosynthesis.
GO - Molecular functioni
- arogenate dehydratase activity Source: UniProtKB
- prephenate dehydratase activity Source: UniProtKB
GO - Biological processi
- L-phenylalanine biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Lyase |
Biological process | Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Phenylalanine biosynthesis |
Enzyme and pathway databases
BRENDAi | 4.2.1.91, 4700 |
UniPathwayi | UPA00121;UER00344 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:ADT31 Publication |
Organismi | Petunia hybrida (Petunia) |
Taxonomic identifieri | 4102 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › asterids › lamiids › Solanales › Solanaceae › Petunioideae › Petunia |
Subcellular locationi
Chloroplast
- chloroplast stroma 1 Publication
Chloroplast
- chloroplast Source: UniProtKB
- chloroplast stroma Source: UniProtKB-SubCell
Keywords - Cellular componenti
Chloroplast, PlastidPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 43 | ChloroplastSequence analysisAdd BLAST | 43 | |
ChainiPRO_0000451504 | 44 – 434 | Arogenate dehydratase 3Add BLAST | 391 |
Expressioni
Tissue specificityi
Expressed at low levels in petals (corollas and tubes), stems, leaves, pistils, stamens, ovaries and sepals.1 Publication
Developmental stagei
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 140 – 317 | Prephenate dehydratasePROSITE-ProRule annotationAdd BLAST | 178 | |
Domaini | 331 – 422 | ACTPROSITE-ProRule annotationAdd BLAST | 92 |
Keywords - Domaini
Transit peptideFamily and domain databases
InterProi | View protein in InterPro IPR002912, ACT_dom IPR001086, Preph_deHydtase IPR018528, Preph_deHydtase_CS |
Pfami | View protein in Pfam PF00800, PDT, 1 hit |
PROSITEi | View protein in PROSITE PS51671, ACT, 1 hit PS00857, PREPHENATE_DEHYDR_1, 1 hit PS00858, PREPHENATE_DEHYDR_2, 1 hit PS51171, PREPHENATE_DEHYDR_3, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
D3U717-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQSLTPSSSG INIKLLNRKK IQTHRVTPSR VVIKCVYRFD SVNAGAAAVN
60 70 80 90 100
TGVVNTPASN NTAGHVGASR TDWQSSCAIL ASKVVSQQQD TEKSGGAGNI
110 120 130 140 150
TAVNGHKILN LDLLPVESNR AKPLTITDLS PAPMHGAQLR VAYQGVPGAY
160 170 180 190 200
SEAAAGKAYP KCEAIPCDQF EVAFQAVELW IADRAVLPIE NSLGGSIHRN
210 220 230 240 250
YDLLLRHRLH IVGEVQLPVH HCLLALPGVR KEYLTRVISH PQALAQCELT
260 270 280 290 300
LTKLGLNVAR EAVDDTAGAA EYIAANNLRD TAAIASSRAA ELYGLDILEQ
310 320 330 340 350
GIQDDLSNVT RFVMLAREPI IPRTDRPFKT SIVFAHDKGT SVLFKVLSAF
360 370 380 390 400
AFRNISLTKI ESRPHRNRPI RLVDDANVGT AKHFEYMFYV DFEASMADVR
410 420 430
AQNALAEVQE FTSFLRVLGS YPMDMTPWSP SRDA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | FJ790414 mRNA Translation: ACY79504.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | FJ790414 mRNA Translation: ACY79504.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Enzyme and pathway databases
UniPathwayi | UPA00121;UER00344 |
BRENDAi | 4.2.1.91, 4700 |
Family and domain databases
InterProi | View protein in InterPro IPR002912, ACT_dom IPR001086, Preph_deHydtase IPR018528, Preph_deHydtase_CS |
Pfami | View protein in Pfam PF00800, PDT, 1 hit |
PROSITEi | View protein in PROSITE PS51671, ACT, 1 hit PS00857, PREPHENATE_DEHYDR_1, 1 hit PS00858, PREPHENATE_DEHYDR_2, 1 hit PS51171, PREPHENATE_DEHYDR_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AROD3_PETHY | |
Accessioni | D3U717Primary (citable) accession number: D3U717 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 2, 2020 |
Last sequence update: | April 20, 2010 | |
Last modified: | December 2, 2020 | |
This is version 22 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |