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Protein

L-lactate dehydrogenase

Gene

ldh

Organism
Thermoanaerobacter italicus (strain DSM 9252 / Ab9)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of lactate to pyruvate.UniRule annotationSAAS annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.UniRule annotation

Enzyme regulationi

Allosterically activated by fructose 1,6-bisphosphate (FBP).UniRule annotation

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase (ldh)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei12NAD; via amide nitrogenUniRule annotation1
Binding sitei33NADUniRule annotation1
Binding sitei38NADUniRule annotation1
Binding sitei63NADUniRule annotation1
Binding sitei80SubstrateUniRule annotation1
Binding sitei86SubstrateUniRule annotation1
Binding sitei118SubstrateUniRule annotation1
Binding sitei141NADUniRule annotation1
Binding sitei149SubstrateUniRule annotation1
Binding sitei151Allosteric activatorUniRule annotation1
Binding sitei166Allosteric activatorUniRule annotation1
Active sitei173Proton acceptorUniRule annotation1
Binding sitei228SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi77 – 78NADUniRule annotation2
Nucleotide bindingi116 – 118NADUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzymeUniRule annotation, OxidoreductaseUniRule annotation
LigandNADUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenaseUniRule annotation (EC:1.1.1.27UniRule annotation)
Short name:
L-LDHUniRule annotation
Gene namesi
Name:ldhUniRule annotation
Ordered Locus Names:Thit_0237Imported
OrganismiThermoanaerobacter italicus (strain DSM 9252 / Ab9)Imported
Taxonomic identifieri580331 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeThermoanaerobacter
Proteomesi
  • UP000001552 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei219PhosphotyrosineUniRule annotation1

Keywords - PTMi

PhosphoproteinUniRule annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi580331.Thit_0237

Structurei

3D structure databases

ProteinModelPortaliD3T631
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 140Ldh_1_NInterPro annotationAdd BLAST138
Domaini143 – 308Ldh_1_CInterPro annotationAdd BLAST166

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni118 – 121Substrate bindingUniRule annotation4
Regioni146 – 149Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C80 Bacteria
COG0039 LUCA
HOGENOMiHOG000213793
KOiK00016
OMAiMDLMQTA
OrthoDBiPOG091H02EQ

Family and domain databases

Gene3Di3.90.110.10, 1 hit
HAMAPiMF_00488 Lactate_dehydrog, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR011304 L-lactate_DH
IPR018177 L-lactate_DH_AS
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
PRINTSiPR00086 LLDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01771 L-LDH-NAD, 1 hit
PROSITEiView protein in PROSITE
PS00064 L_LDH, 1 hit

Sequencei

Sequence statusi: Complete.

D3T631-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKISIIGSG FVGATTAYTL ALSGFVKTIV LIDINKDKAE GDALDISHGV
60 70 80 90 100
PFISPVEVYA GDYGDAAGSD IIIITAGAAQ KPGETRLDLV KRNTMIFKDI
110 120 130 140 150
VAKLIKVNDT AIYLIVTNPV DILTYVTYKI SGLPYGRVLG SGTVLDSARF
160 170 180 190 200
RYLLSKHCNI DPRNIHGYII GEHGDSELAA WSITNIAGIP IDDYCNLCGK
210 220 230 240 250
ACEKDFRDEI FNDVVRAAYT IIEKKGATYY AVALAVKRIV EAILKDEHSI
260 270 280 290 300
LTVSSPLTGQ YGVTNVALSL PSVVGRNGIV NILELPLSQE EIDAFRRSAE
310
IIKGVIQELD I
Length:311
Mass (Da):33,638
Last modified:April 20, 2010 - v1
Checksum:i7C1AA069EE0855B5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001936 Genomic DNA Translation: ADD01562.1
RefSeqiWP_012994395.1, NC_013921.1

Genome annotation databases

EnsemblBacteriaiADD01562; ADD01562; Thit_0237
KEGGitit:Thit_0237

Similar proteinsi

Entry informationi

Entry nameiD3T631_THEIA
AccessioniPrimary (citable) accession number: D3T631
Entry historyiIntegrated into UniProtKB/TrEMBL: April 20, 2010
Last sequence update: April 20, 2010
Last modified: June 20, 2018
This is version 56 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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