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Protein

2-oxoglutarate carboxylase large subunit

Gene

cfiA

Organism
Hydrogenobacter thermophilus (strain DSM 6534 / IAM 12695 / TK-6)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 2-oxoglutarate + HCO3- = ADP + phosphate + oxalosuccinate.1 Publication

Cofactori

Mg2+By similarity, Mn2+By similarity, Co2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi35Divalent metal cationBy similarity1
Binding sitei105SubstrateBy similarity1
Metal bindingi196Divalent metal cation; via carbamate groupBy similarity1
Metal bindingi227Divalent metal cationBy similarity1
Metal bindingi229Divalent metal cationBy similarity1
Binding sitei362SubstrateBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionLigase
LigandATP-binding, Biotin, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciHTHE608538:G1GOC-1412-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
2-oxoglutarate carboxylase large subunitImported (EC:6.4.1.7)
Alternative name(s):
2-oxoglutarate carboxylase alpha subunit1 Publication
Gene namesi
Name:cfiAImported
Ordered Locus Names:HTH_1392, Hydth_1382
OrganismiHydrogenobacter thermophilus (strain DSM 6534 / IAM 12695 / TK-6)
Taxonomic identifieri608538 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeHydrogenobacter
Proteomesi
  • UP000002574 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004027971 – 6522-oxoglutarate carboxylase large subunitAdd BLAST652

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei196N6-carboxylysineBy similarity1
Modified residuei609N6-biotinyllysinePROSITE-ProRule annotationBy similarity1

Post-translational modificationi

Biotinylated.1 Publication

Interactioni

Subunit structurei

Heterohexadecamer of 8 large subunits and 8 small subunits.2 Publications

Protein-protein interaction databases

STRINGi608538.HTH_1392

Structurei

3D structure databases

ProteinModelPortaliD3DJ41
SMRiD3DJ41
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 288Pyruvate carboxyltransferasePROSITE-ProRule annotationAdd BLAST263
Domaini563 – 643Biotinyl-bindingPROSITE-ProRule annotationAdd BLAST81

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni34 – 38Substrate bindingBy similarity5

Phylogenomic databases

eggNOGiENOG4107QSA Bacteria
COG0511 LUCA
COG5016 LUCA
HOGENOMiHOG000231389
KOiK20140
OMAiELHLHCH
OrthoDBiPOG091H0184

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR001882 Biotin_BS
IPR000089 Biotin_lipoyl
IPR003379 Carboxylase_cons_dom
IPR000891 PYR_CT
IPR011053 Single_hybrid_motif
PfamiView protein in Pfam
PF00364 Biotin_lipoyl, 1 hit
PF00682 HMGL-like, 1 hit
PF02436 PYC_OADA, 1 hit
SUPFAMiSSF51230 SSF51230, 1 hit
PROSITEiView protein in PROSITE
PS00188 BIOTIN, 1 hit
PS50968 BIOTINYL_LIPOYL, 1 hit
PS50991 PYR_CT, 1 hit

Sequencei

Sequence statusi: Complete.

D3DJ41-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQAVEIMEEI REKFKEFEKG GFRKKILITD LTPRDGQQCK LATRVRTDDL
60 70 80 90 100
LPLCEAMDKV GFYAVEVWGG ATYDVCLRYL KEDPWERLRR IKEVMPNTKL
110 120 130 140 150
QMLFRGQNIV GYRPKSDKLV YKFVERAIKN GITVFRVFDA LNDNRNIKTA
160 170 180 190 200
VKAIKELGGE AHAEISYTRS PIHTYQKWIE YALEIAEMGA DWLSFKDATG
210 220 230 240 250
IIMPFETYAI IKGIKEATGG KLPVLLHNHD MSGTAIVNHM MAVLAGVDML
260 270 280 290 300
DTVLSPLAFG SSHPATESVV AMLEGTPFDT GIDMKKLDEL AEIVKQIRKK
310 320 330 340 350
YKKYETEYAG VNAKVLIHKI PGGMISNMVA QLIEANALDK IEEALEEVPN
360 370 380 390 400
VERDLGHPPL LTPSSQIVGV QAVLNVISGE RYKVITKEVR DYVEGKYGKP
410 420 430 440 450
PGPISKELAE KILGPGKEPD FSIRAADLAD PNDWDKAYEE TKAILGREPT
460 470 480 490 500
DEEVLLYALF PMQAKDFFVA REKGELHPEP VDELVETTEV KAGVVPGAAP
510 520 530 540 550
VEFEIVYHGE KFKVKVEGVS AHQEPGKPRK YYIRVDGRLE EVQITPHVEA
560 570 580 590 600
IPKGGPTPTA VQAEEKGIPK ATQPGDATAP MPGRVVRVLV KEGDKVKEGQ
610 620 630 640 650
TVAIVEAMKM ENEIHAPISG VVEKVFVKPG DNVTPDDALL RIKHIEEEVS

YG
Length:652
Mass (Da):72,546
Last modified:March 23, 2010 - v1
Checksum:i8D6C35B018DF9921
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB246889 Genomic DNA Translation: BAF34932.1
AP011112 Genomic DNA Translation: BAI69843.1
CP002221 Genomic DNA Translation: ADO45767.1
RefSeqiWP_012964023.1, NC_017161.1

Genome annotation databases

EnsemblBacteriaiADO45767; ADO45767; Hydth_1382
BAI69843; BAI69843; HTH_1392
KEGGihte:Hydth_1382
hth:HTH_1392
PATRICifig|608538.5.peg.1413

Similar proteinsi

Entry informationi

Entry namei2OCL_HYDTT
AccessioniPrimary (citable) accession number: D3DJ41
Secondary accession number(s): Q05KD8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: March 23, 2010
Last modified: February 28, 2018
This is version 50 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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