UniProtKB - D2TPI3 (D2TPI3_CITRI)
Protein
Adenosylmethionine-8-amino-7-oxononanoate aminotransferase
Gene
bioA
Organism
Citrobacter rodentium (strain ICC168) (Citrobacter freundii biotype 4280)
Status
Functioni
Catalyzes the transfer of the alpha-amino group from S-adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA). It is the only aminotransferase known to utilize SAM as an amino donor.UniRule annotation
Catalytic activityi
- (S)-8-amino-7-oxononanoate + S-adenosyl-L-methionine = (7R,8S)-7,8-diammoniononanoate + S-adenosyl-4-methylsulfanyl-2-oxobutanoateUniRule annotationEC:2.6.1.62UniRule annotation
Cofactori
pyridoxal 5'-phosphateUniRule annotationARBA annotation
: biotin biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes 7,8-diaminononanoate from 8-amino-7-oxononanoate (SAM route).UniRule annotationProteins known to be involved in this subpathway in this organism are:
- Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (bioA)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 7,8-diaminononanoate from 8-amino-7-oxononanoate (SAM route), the pathway biotin biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 17 | Participates in the substrate recognition with KAPA and in a stacking interaction with the adenine ring of SAMUniRule annotation | 1 | |
Binding sitei | 52 | SubstrateUniRule annotation | 1 | |
Binding sitei | 144 | SubstrateUniRule annotation | 1 | |
Binding sitei | 245 | Pyridoxal phosphateUniRule annotation | 1 | |
Binding sitei | 274 | SubstrateUniRule annotation | 1 | |
Binding sitei | 307 | Substrate; via carbonyl oxygenUniRule annotation | 1 | |
Binding sitei | 391 | SubstrateUniRule annotation | 1 |
GO - Molecular functioni
- adenosylmethionine-8-amino-7-oxononanoate transaminase activity Source: UniProtKB-UniRule
- pyridoxal phosphate binding Source: UniProtKB-UniRule
GO - Biological processi
- biotin biosynthetic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | AminotransferaseUniRule annotationImported, Transferase |
Biological process | Biotin biosynthesisUniRule annotation |
Ligand | Pyridoxal phosphateUniRule annotationARBA annotation, S-adenosyl-L-methionineUniRule annotationARBA annotation |
Enzyme and pathway databases
BioCyci | CROD637910:G1GIT-878-MONOMER |
UniPathwayi | UPA00078;UER00160 |
Names & Taxonomyi
Protein namesi | Recommended name: Adenosylmethionine-8-amino-7-oxononanoate aminotransferaseUniRule annotation (EC:2.6.1.62UniRule annotation)Alternative name(s): 7,8-diamino-pelargonic acid aminotransferaseUniRule annotation Short name: DAPA ATUniRule annotation Short name: DAPA aminotransferaseUniRule annotation 7,8-diaminononanoate synthaseUniRule annotation Short name: DANSUniRule annotation Diaminopelargonic acid synthaseUniRule annotation |
Gene namesi | Name:bioAUniRule annotationImported Ordered Locus Names:ROD_07751Imported |
Organismi | Citrobacter rodentium (strain ICC168) (Citrobacter freundii biotype 4280)Imported |
Taxonomic identifieri | 637910 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Citrobacter › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmUniRule annotationPTM / Processingi
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 274 | N6-(pyridoxal phosphate)lysineUniRule annotation | 1 |
Interactioni
Subunit structurei
Homodimer.
UniRule annotationProtein-protein interaction databases
STRINGi | 637910.ROD_07751 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 112 – 113 | Pyridoxal phosphate bindingUniRule annotation | 2 | |
Regioni | 308 – 309 | Pyridoxal phosphate bindingUniRule annotation | 2 |
Sequence similaritiesi
Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. BioA subfamily.UniRule annotation
Phylogenomic databases
eggNOGi | COG0161, Bacteria |
HOGENOMi | CLU_016922_4_3_6 |
OMAi | GVWLEDF |
OrthoDBi | 478143at2 |
Family and domain databases
CDDi | cd00610, OAT_like, 1 hit |
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
HAMAPi | MF_00834, BioA, 1 hit |
InterProi | View protein in InterPro IPR005814, Aminotrans_3 IPR005815, BioA IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
Pfami | View protein in Pfam PF00202, Aminotran_3, 1 hit |
PIRSFi | PIRSF000521, Transaminase_4ab_Lys_Orn, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
TIGRFAMsi | TIGR00508, bioA, 1 hit |
PROSITEi | View protein in PROSITE PS00600, AA_TRANSFER_CLASS_3, 1 hit |
i Sequence
Sequence statusi: Complete.
D2TPI3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTTDDLAFDR RHIWHPYTSM TSPLPVFPVE RAEGCELILS GGERLVDGMS
60 70 80 90 100
SWWAAIHGYN HPQLNAAMKT QIDAMSHVMF GGITHAPAIE LCRKLVAMTP
110 120 130 140 150
APLECVFLAD SGSVAVEVAM KMALQYWQAK GESRQRFLTF RNGYHGDTFG
160 170 180 190 200
AMSVCDPHNS MHSLWKGYLA ENLFAPAPQS RMGGEWDERD MVAFARLMAA
210 220 230 240 250
HRHEIAAVIL EPIVQGAGGM RIYHPQWLQR IRQMCDREGI LLIADEIATG
260 270 280 290 300
FGRTGRLFAC EHAGIAPDIL CLGKALTGGT MTLSATLTSR QVAETISNGE
310 320 330 340 350
AGCFMHGPTF MGNPLACAVA NASLTLLESG DWRRQVAAIE AQLREELAPA
360 370 380 390 400
ADSPQVADVR VLGAIGVVET TRPVNIAALQ QFFVRQGVWV RPFGKLIYLM
410 420
PPYIIQPEQL RRLTQAVNEA VREETFFSV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | FN543502 Genomic DNA Translation: CBG87552.1 |
RefSeqi | WP_012905104.1, NC_013716.1 |
Genome annotation databases
EnsemblBacteriai | CBG87552; CBG87552; ROD_07751 |
KEGGi | cro:ROD_07751 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | FN543502 Genomic DNA Translation: CBG87552.1 |
RefSeqi | WP_012905104.1, NC_013716.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5UC7 | X-ray | 1.83 | A | 1-429 | [»] | |
SMRi | D2TPI3 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 637910.ROD_07751 |
Genome annotation databases
EnsemblBacteriai | CBG87552; CBG87552; ROD_07751 |
KEGGi | cro:ROD_07751 |
Phylogenomic databases
eggNOGi | COG0161, Bacteria |
HOGENOMi | CLU_016922_4_3_6 |
OMAi | GVWLEDF |
OrthoDBi | 478143at2 |
Enzyme and pathway databases
UniPathwayi | UPA00078;UER00160 |
BioCyci | CROD637910:G1GIT-878-MONOMER |
Family and domain databases
CDDi | cd00610, OAT_like, 1 hit |
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
HAMAPi | MF_00834, BioA, 1 hit |
InterProi | View protein in InterPro IPR005814, Aminotrans_3 IPR005815, BioA IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
Pfami | View protein in Pfam PF00202, Aminotran_3, 1 hit |
PIRSFi | PIRSF000521, Transaminase_4ab_Lys_Orn, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
TIGRFAMsi | TIGR00508, bioA, 1 hit |
PROSITEi | View protein in PROSITE PS00600, AA_TRANSFER_CLASS_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | D2TPI3_CITRI | |
Accessioni | D2TPI3Primary (citable) accession number: D2TPI3 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | March 2, 2010 |
Last sequence update: | March 2, 2010 | |
Last modified: | December 2, 2020 | |
This is version 75 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |