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Protein

Cytotoxic L-amino-acid oxidase

Gene
N/A
Organism
Amanita phalloides (Death cap)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cytotoxic L-amino acid oxidase with high oxidase activity towards DL-methionine and L-methionine, L-phenylalanine, DL-norleucine, L-isoleucine, L-arginine, L-tyrosine, and DL-leucine. Shows relatively low activity towards DL-lysine and L-lysine, DL-asparagine, DL-valine, L-histidine, DL-threonine, DL-thryptophane, and L-glutamic acid; and no activity towards L-cysteine, L-glycine, L-proline, L-oxyproline, DL-serine, and DL-aspartic acid. Does not use benzylamine, ethanolamine, diethylamine, meta- and para-phenylendiamine, ortho-, meta- and para-aminophenols, or putrescin as a substrate. Acts as a toxin by inducing chromatin condensation, as well as DNA and nucleus fragmentation, which are typical for apoptosis (PubMed:20121947). Probably induces cell damage indirectly via the generation of free radicals and oxidant agents that can trigger cell impairment and apoptosis by a caspase-independent pathway (PubMed:20121947).1 Publication

Miscellaneous

Ascorbic acid inhibits the cytotoxic effect caused by toxophallin (PubMed:20121947). The mechanisms of such inhibition could be based on inactivating the H2O2 that appears as a result of the amine oxidase reaction and is toxic for cells (PubMed:20121947).1 Publication

Catalytic activityi

An L-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.1 Publication

Cofactori

FADCurated

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase, Toxin
LigandFAD, Flavoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Cytotoxic L-amino-acid oxidase1 Publication (EC:1.4.3.21 Publication)
Alternative name(s):
Toxophallin1 Publication
OrganismiAmanita phalloides (Death cap)
Taxonomic identifieri67723 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesAgaricomycetidaeAgaricalesAmanitaceaeAmanita

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000443561‹1 – ›143Cytotoxic L-amino-acid oxidaseAdd BLAST›143

Family & Domainsi

Sequence similaritiesi

Belongs to the flavin monoamine oxidase family.Curated

Sequencei

Sequence statusi: Fragment.

D2KKB4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
RVFHLFDYPP LNTGDLQLKA KIKPFIFTSN NSFLSYNDVT VKHNDVPAGD
60 70 80 90 100
PFKASAVIKD TNPNPYIAAG VTAILNDVLG RFAVPLMNDL KTGKTDGWDL
110 120 130 140
MMKYDKHSTR SYMALAYTPS DHLNLPKKPL PTDVINWLET FDK
Length:143
Mass (Da):16,087
Last modified:February 9, 2010 - v1
Checksum:i5D91CA7EDFF0581B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei1Curated1
Non-terminal residuei143Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GU220069 mRNA Translation: ADA58360.1

Entry informationi

Entry nameiTOXPH_AMAPH
AccessioniPrimary (citable) accession number: D2KKB4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 28, 2018
Last sequence update: February 9, 2010
Last modified: April 25, 2018
This is version 6 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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