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UniProtKB - D1MI46 (C76BA_SWEMU)
Protein
Geraniol 8-hydroxylase
Gene
CYP76B10
Organism
Swertia mussotii (Felwort)
Status
Functioni
Hydroxylase involved in the biosynthesis of hydroxygeraniol, a precursor of the iridoid monoterpenoid swertiamarin.
1 PublicationMiscellaneous
The recommended numbering of geraniol gives (6E)-8-hydroxygeraniol as the product rather than 10-hydroxygeraniol as used in most publications.
Catalytic activityi
- (2E)-geraniol + O2 + reduced [NADPH—hemoprotein reductase] = (6E)-8-hydroxygeraniol + H+ + H2O + oxidized [NADPH—hemoprotein reductase]1 PublicationEC:1.14.14.831 Publication
Cofactori
hemeBy similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 438 | Iron (heme axial ligand)By similarity | 1 |
GO - Molecular functioni
- geraniol 10-hydroxylase activity Source: UniProtKB-EC
- heme binding Source: InterPro
- iron ion binding Source: InterPro
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen Source: UniProtKB
GO - Biological processi
- monoterpenoid biosynthetic process Source: UniProtKB
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Ligand | Heme, Iron, Metal-binding, NADP |
Enzyme and pathway databases
BRENDAi | 1.14.14.83, 12614 |
Names & Taxonomyi
Protein namesi | Recommended name: Geraniol 8-hydroxylase (EC:1.14.14.831 Publication)Alternative name(s): Cytochrome P450 76B10 Geraniol 10-hydroxylase Short name: SmG10H |
Gene namesi | Name:CYP76B10 Synonyms:G10H |
Organismi | Swertia mussotii (Felwort) |
Taxonomic identifieri | 137888 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › asterids › lamiids › Gentianales › Gentianaceae › Gentianeae › Swertiinae › Swertia |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane Curated; Single-pass membrane protein Curated
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 5 – 25 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000418920 | 1 – 495 | Geraniol 8-hydroxylaseAdd BLAST | 495 |
Proteomic databases
PRIDEi | D1MI46 |
Expressioni
Tissue specificityi
Expressed in leaves, stems and roots.1 Publication
Inductioni
Up-regulated by methyl jasmonate treatment.1 Publication
Family & Domainsi
Sequence similaritiesi
Belongs to the cytochrome P450 family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixFamily and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
i Sequence
Sequence statusi: Complete.
D1MI46-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDFDFLTIAI GFLFTITLYQ ALNFFSRKSK NLPPGPSPLP LIGNLHLLGD
60 70 80 90 100
QPHKSLAKLA KKHGPIMGLQ LGQVTTIVVT SSGMAKEVLQ KQDLAFSSRS
110 120 130 140 150
IPNAIHAHDQ YKYSVIWLPV ASRWRGLRKA LNSNMFSGNR LDANQHLRSR
160 170 180 190 200
KVQELIAYCR KSSQTGDAID VGRAAFRTSL NLLSNTMFSK DLTDPYSDSA
210 220 230 240 250
KEFKDLVWNV MVEAGKPNLV DYFPLLDKVD PQGIRKRMTI HFGKILELFG
260 270 280 290 300
GLIDERLQQK KAKGVNDDVL DVLLTTSEES PEEIDRTHIQ RMCLDLFVAG
310 320 330 340 350
TDTTSSTLEW AMSEMLKNPE KMKAAQAELA QVIGKGKAVE EADLARLPYL
360 370 380 390 400
RCAIKETLRI HPPVPLLIPR RTEQEVEVCG YTVPKNSQVL VNVWAISRDD
410 420 430 440 450
AIWKDPLSFK PERFLESELE MRGKDFELIP FGAGRRICPG LPLAVRMVPV
460 470 480 490
MLGSLLNSFD WKLEGGIAPK DLDMEEKFGI TLQKAHPLRA VATPL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | GU168041 mRNA Translation: ACZ48680.1 |
Genome annotation databases
KEGGi | ag:ACZ48680 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | GU168041 mRNA Translation: ACZ48680.1 |
3D structure databases
SMRi | D1MI46 |
ModBasei | Search... |
Proteomic databases
PRIDEi | D1MI46 |
Genome annotation databases
KEGGi | ag:ACZ48680 |
Enzyme and pathway databases
BRENDAi | 1.14.14.83, 12614 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | C76BA_SWEMU | |
Accessioni | D1MI46Primary (citable) accession number: D1MI46 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 5, 2012 |
Last sequence update: | January 19, 2010 | |
Last modified: | June 2, 2021 | |
This is version 42 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |