UniProtKB - D1LVD8 (D1LVD8_BPT7)
Protein
Endolysin
Gene
3.5
Organism
Escherichia phage T7 (Bacteriophage T7)
Status
Functioni
Endolysin with amidase activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and breaking down the peptidoglycan layer.UniRule annotation
Plays an important role in the switch between viral transcription and genome replication. Once produced in sufficient amount, interacts with and inhibits the viral RNA polymerase that becomes unable to produce additional late transcripts. This lysozyme-polymerase complex in turn plays an active role in viral genome replication and packaging.UniRule annotation
Catalytic activityi
- Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.UniRule annotation EC:3.5.1.28
Cofactori
Zn2+UniRule annotationNote: Zn2+ is required for amidase activity.UniRule annotation
Activity regulationi
Binding to the viral RNA polymerase inhibits amidase activity.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 18 | ZincUniRule annotation | 1 | |
Sitei | 47 | Essential for amidase activity and zinc hydrate coordinationUniRule annotation | 1 | |
Metal bindingi | 123 | ZincUniRule annotation | 1 | |
Metal bindingi | 131 | ZincUniRule annotation | 1 |
GO - Molecular functioni
- N-acetylmuramoyl-L-alanine amidase activity Source: UniProtKB-UniRule
- zinc ion binding Source: InterPro
GO - Biological processi
- cytolysis Source: UniProtKB-KW
- defense response to bacterium Source: UniProtKB-KW
- negative regulation of viral transcription Source: InterPro
- peptidoglycan catabolic process Source: UniProtKB-UniRule
- viral release from host cell by cytolysis Source: UniProtKB-UniRule
Keywordsi
Molecular function | Antimicrobial, Bacteriolytic enzymeUniRule annotationARBA annotation, HydrolaseUniRule annotation |
Biological process | Cytolysis, Host cell lysis by virusUniRule annotation, Viral release from host cell |
Ligand | Metal-bindingUniRule annotation, ZincUniRule annotation |
Names & Taxonomyi
Protein namesi | Recommended name: EndolysinUniRule annotation (EC:3.5.1.28UniRule annotation)Alternative name(s): N-acetylmuramoyl-L-alanine amidaseUniRule annotation |
Gene namesi | Name:3.5Imported ORF Names:CEPIPMPB_00019Imported, EPTG_00019Imported, KIHBJNID_00019Imported, mT7_p20Imported, OCKDAKLP_00019Imported, OECHGEMH_00019Imported, POOFLLJK_00019Imported |
Organismi | Escherichia phage T7 (Bacteriophage T7)Imported |
Taxonomic identifieri | 10760 [NCBI] |
Taxonomic lineagei | Viruses › Duplodnaviria › Heunggongvirae › Uroviricota › Caudoviricetes › Caudovirales › Autographiviridae › Studiervirinae › Teseptimavirus › |
Virus hosti | Escherichia coli [TaxID: 562] |
Proteomesi |
|
Subcellular locationi
- Host cytoplasm UniRule annotation Note: The endolysin is cytoplasmic, but can reach the periplasmic space with the help of the holins which disrupt the host cell membrane.UniRule annotation
GO - Cellular componenti
- host cell cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
Host cytoplasmUniRule annotationInteractioni
Subunit structurei
Interacts with the viral RNA polymerase.
UniRule annotationFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 151 | N-acetylmuramoyl-L-alanine amidaseInterPro annotationAdd BLAST | 151 | |
Domaini | 1 – 124 | PGRPInterPro annotationAdd BLAST | 124 |
Sequence similaritiesi
Family and domain databases
CDDi | cd06583, PGRP, 1 hit |
Gene3Di | 3.40.80.10, 1 hit |
HAMAPi | MF_04111, ENDOLYSIN_T7, 1 hit |
InterProi | View protein in InterPro IPR036505, Amidase/PGRP_sf IPR002502, Amidase_domain IPR034689, Endolysin_T7_type IPR015510, PGRP IPR006619, PGRP_domain_met/bac |
PANTHERi | PTHR11022, PTHR11022, 1 hit |
Pfami | View protein in Pfam PF01510, Amidase_2, 1 hit |
SMARTi | View protein in SMART SM00644, Ami_2, 1 hit SM00701, PGRP, 1 hit |
SUPFAMi | SSF55846, SSF55846, 1 hit |
i Sequence
Sequence statusi: Complete.
D1LVD8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MARVQFKQRE STDAIFVHCS ATKPSQNVGV REIRQWHKEQ GWLDVGYHFI
60 70 80 90 100
IKRDGTVEAG RDEMAVGSHA KGYNHNSIGV CLVGGIDDKG KFDANFTPAQ
110 120 130 140 150
MQSLRSLLVT LLAKYEGAVL RAHHEVAPKA CPSFDLKRWW EKNELVTSDR
G
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | GU071091 Genomic DNA Translation: ACY75847.1 MG833025 Genomic DNA Translation: AVH85534.1 LR745706 Genomic DNA Translation: CAA6798263.1 LR745709 Genomic DNA Translation: CAA6799837.1 LR745710 Genomic DNA Translation: CAA6800430.1 LR745708 Genomic DNA Translation: CAA6800474.1 LR745707 Genomic DNA Translation: CAA6830954.1 |
RefSeqi | NP_041973.1, NC_001604.1 |
Genome annotation databases
GeneIDi | 1261077 |
KEGGi | vg:1261077 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | GU071091 Genomic DNA Translation: ACY75847.1 MG833025 Genomic DNA Translation: AVH85534.1 LR745706 Genomic DNA Translation: CAA6798263.1 LR745709 Genomic DNA Translation: CAA6799837.1 LR745710 Genomic DNA Translation: CAA6800430.1 LR745708 Genomic DNA Translation: CAA6800474.1 LR745707 Genomic DNA Translation: CAA6830954.1 |
RefSeqi | NP_041973.1, NC_001604.1 |
3D structure databases
SMRi | D1LVD8 |
ModBasei | Search... |
Genome annotation databases
GeneIDi | 1261077 |
KEGGi | vg:1261077 |
Family and domain databases
CDDi | cd06583, PGRP, 1 hit |
Gene3Di | 3.40.80.10, 1 hit |
HAMAPi | MF_04111, ENDOLYSIN_T7, 1 hit |
InterProi | View protein in InterPro IPR036505, Amidase/PGRP_sf IPR002502, Amidase_domain IPR034689, Endolysin_T7_type IPR015510, PGRP IPR006619, PGRP_domain_met/bac |
PANTHERi | PTHR11022, PTHR11022, 1 hit |
Pfami | View protein in Pfam PF01510, Amidase_2, 1 hit |
SMARTi | View protein in SMART SM00644, Ami_2, 1 hit SM00701, PGRP, 1 hit |
SUPFAMi | SSF55846, SSF55846, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | D1LVD8_BPT7 | |
Accessioni | D1LVD8Primary (citable) accession number: D1LVD8 Secondary accession number(s): A0A2P0ZKL2 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | January 19, 2010 |
Last sequence update: | January 19, 2010 | |
Last modified: | August 12, 2020 | |
This is version 51 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |