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Protein

Isoamylase 1, chloroplastic

Gene

ISA1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Starch-debranching enzyme involved in amylopectin biosynthesis in endosperm. Functions by removing excess branches or improper branches that interfere with the formation of double helices of the cluster chains of amylopectin and crystallization of starch (PubMed:10517831, PubMed:15618430, PubMed:16953433, PubMed:21436381). Works as ISA1 homooligomer or together with ISA2 as heterooligomer. The heterooligomer ISA1 and ISA2 possesses higher affinity than the ISA1 homooligomer for various branched polyglucans in vitro, but no marked differences exist in chain preferences for debranching of amylopectin and phytoglycogen between these forms (PubMed:16953433, PubMed:21436381).4 Publications

Catalytic activityi

Hydrolysis of (1->6)-alpha-D-glucosidic branch linkages in glycogen, amylopectin and their beta-limit dextrins.1 Publication

Enzyme regulationi

Inhibited by copper chloride, mercury chloride, ammonium molybdate and para-chloromercuribenzoate.1 Publication

pH dependencei

Optimum pH is 6.5 at 30 degrees Celsius.1 Publication

Pathwayi: starch biosynthesis

This protein is involved in the pathway starch biosynthesis, which is part of Glycan biosynthesis.Curated
View all proteins of this organism that are known to be involved in the pathway starch biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei432NucleophileBy similarity1
Active sitei488Proton donorBy similarity1
Sitei561Transition state stabilizerBy similarity1

GO - Molecular functioni

  • isoamylase activity Source: UniProtKB

GO - Biological processi

  • amylopectin biosynthetic process Source: UniProtKB
  • starch biosynthetic process Source: UniProtKB-UniPathway
  • starch catabolic process Source: UniProtKB

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Starch biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00152

Names & Taxonomyi

Protein namesi
Recommended name:
Isoamylase 1, chloroplasticCurated (EC:3.2.1.681 Publication)
Short name:
OsISA11 Publication
Alternative name(s):
Protein SUGARY-11 Publication
Gene namesi
Name:ISA11 Publication
Synonyms:ISA1 Publication, SU11 Publication
Ordered Locus Names:Os08g0520900Imported, LOC_Os08g40930Curated
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Replacement of endosperm starch by a water-soluble highly and randomly branched polysaccharide structure called phytoglycogen.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 54Chloroplast1 PublicationAdd BLAST54
ChainiPRO_000044179855 – 803Isoamylase 1, chloroplasticAdd BLAST749

Expressioni

Tissue specificityi

Highly expressed in developing endosperm. Expressed at low levels in leaves.1 Publication

Gene expression databases

ExpressionAtlasiD0TZF0 differential

Interactioni

Subunit structurei

Forms a homo-pentamer and a hetero-hexamer composed of five ISA1 and one ISA2 (PubMed:16953433). Interacts with FLO6/SIP4 (PubMed:24456533).2 Publications

Structurei

3D structure databases

SMRiD0TZF0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi61 – 80Glu-richPROSITE-ProRule annotationAdd BLAST20

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IN7N Eukaryota
COG1523 LUCA
KOiK01214

Family and domain databases

Gene3Di2.60.40.10, 1 hit
2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006047 Glyco_hydro_13_cat_dom
IPR004193 Glyco_hydro_13_N
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02922 CBM_48, 1 hit
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SUPFAMiSSF51445 SSF51445, 3 hits
SSF81296 SSF81296, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

D0TZF0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASLPHCLSA RPLVVAAAPG RPGPGPGPWL RGGARRRNAA FSAGNAGRRV
60 70 80 90 100
GLRRSVASAV EVGVGEDEEE GVEEEEEEVE AVVMPERYAL GGACRVLAGM
110 120 130 140 150
PAPLGATALD GGVNFAVYSA GASAASLCLF TPDDLEADEV TEEVPLDPLF
160 170 180 190 200
NRTGNVWHVF IEGELHNMLY GYRFDGMFAP HCGQYFDVSN VVVDPYAKAV
210 220 230 240 250
ISRGEYGVPG PGGDCWPQMA GMIPLPYSTF DWQGDLPLRY PQKDLVIYEM
260 270 280 290 300
HLRGFTKHSS SNVEHPGTYI GAISKLDYLK ELGVNCVELM PCHEFNELEY
310 320 330 340 350
FSCSSKMNFW GYSTINFFSP MIRYSSGGIR NCGRDAINEF KTFVREAHKR
360 370 380 390 400
GIEVIMDVVF NHTAEGNEKG PILSFRGIDN STYYMLAPKG EFYNYSGCGN
410 420 430 440 450
TFNCNHPVVR EFIVDCLRYW VTEMHVDGFR FDLASIMTRG CSLWDPVNVY
460 470 480 490 500
GSPVEGDMTT TGTPLATPPL IDMISNDPIL GDVKLIAEAW DAGGLYQVGQ
510 520 530 540 550
FPHWKIWSEW NGKYRDIVRQ FIKGTDGFAG GFAECLCGSP HLYQAGGRKP
560 570 580 590 600
WHSINFVCAH DGFTLADLVT YNKKYNSSNG EDNRDGENHN LSWNCGEEGE
610 620 630 640 650
FAGLSVKRLR KRQMRNFFVS LMVSQGVPMF YMGDEYGHTK GGNNNTYCHD
660 670 680 690 700
HYVNYFRWDK KEESSDLQRF CSLMTKFRKQ CESLGLADFP TAQRLHWHGH
710 720 730 740 750
QPGKPDWSET SRFVAFSTKD ETKGEIYVAF NASHLPAVVG LPERPGYRWE
760 770 780 790 800
PLVDTGKPAP YDFLTDDLPD RAHAVHLFSH FLNSNLYPML SYSSIILELQ

PDD
Length:803
Mass (Da):89,661
Last modified:December 15, 2009 - v1
Checksum:i9DC80176A72292E0
GO

Sequence cautioni

The sequence BAA29041 differs from that shown. Reason: Frameshift at position 773.Curated
The sequence BAC75533 differs from that shown. Sequencing errors.Curated
The sequence BAF24185 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAT06299 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB093426 Genomic DNA Translation: BAC75533.1 Sequence problems.
GQ150871 Genomic DNA Translation: ACY56086.1
GQ150872 Genomic DNA Translation: ACY56087.1
GQ150873 Genomic DNA Translation: ACY56088.1
GQ150874 Genomic DNA Translation: ACY56089.1
GQ150875 Genomic DNA Translation: ACY56090.1
GQ150876 Genomic DNA Translation: ACY56091.1
KF984388 Genomic DNA Translation: AII21927.1
AP008214 Genomic DNA Translation: BAF24185.1 Different initiation.
AP014964 Genomic DNA Translation: BAT06299.1 Different initiation.
AB015615 mRNA Translation: BAA29041.1 Frameshift.
RefSeqiXP_015650242.1, XM_015794756.1

Genome annotation databases

EnsemblPlantsiOs08t0520900-00; Os08t0520900-00; Os08g0520900
GeneIDi4346068
GrameneiOs08t0520900-00; Os08t0520900-00; Os08g0520900
KEGGiosa:4346068

Similar proteinsi

Entry informationi

Entry nameiISOA1_ORYSJ
AccessioniPrimary (citable) accession number: D0TZF0
Secondary accession number(s): O80403, Q0J4C6, Q84L53
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2017
Last sequence update: December 15, 2009
Last modified: June 20, 2018
This is version 57 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  4. SIMILARITY comments
    Index of protein domains and families

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