UniProtKB - D0PV95 (DDX3_CAEEL)
ATP-dependent RNA helicase laf-1
laf-1
Functioni
Multifunctional ATP-dependent RNA helicase (PubMed:27546789).
Plays a role in RNA remodeling, but is not required for RNA unwinding (PubMed:27546789).
Binds to RNA in a concentration-dependent manner to stimulate annealing between two complementary strands of RNA (PubMed:26015579, PubMed:27546789).
This process is also dependent upon ATP; ATP reduces binding to RNA and subsequently diminishes RNA annealing (PubMed:27546789).
Involved in many cellular processes, which do not necessarily require its ATPase/helicase catalytic activities. Involved in the regulation of transcription and translation initiation. Involved in innate immunity (By similarity).
Involved in both stress and inflammatory responses (By similarity).
Promotes liquid-liquid phase separation of P granules, which is a process important for intracellular organization and stress granule assembly (PubMed:26015579).
Required for embryonic development (PubMed:19361491, PubMed:26015579).
Plays a role in sexual cell fate determination by negatively regulating the translation of the sex determining protein tra-2 (PubMed:9043090, PubMed:9321409, PubMed:26015579).
May play a protective role in the response to heat and oxidative stress (PubMed:24844228).
May negatively regulate extrinsic apoptotic signaling pathway via death domain receptors. May be involved in mitotic chromosome segregation (By similarity).
By similarity6 PublicationsCatalytic activityi
- EC:3.6.4.131 Publication
Kineticsi
- KM=1.3156 mM for ATP1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 275 – 282 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ATP hydrolysis activity Source: RHEA
- identical protein binding Source: UniProtKB
- RNA binding Source: GO_Central
- RNA helicase activity Source: GO_Central
- RNA strand annealing activity Source: UniProtKB
GO - Biological processi
- cell differentiation Source: GO_Central
- gamete generation Source: GO_Central
- masculinization of hermaphroditic germ-line Source: UniProtKB
- negative regulation of gene expression Source: UniProtKB
- positive regulation of embryonic development Source: UniProtKB
- positive regulation of fertilization Source: UniProtKB
- regulation of translation Source: UniProtKB-KW
Keywordsi
Molecular function | Helicase, Hydrolase, RNA-binding |
Biological process | Transcription, Transcription regulation, Translation regulation |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: ATP-dependent RNA helicase laf-1Curated (EC:3.6.4.131 Publication)Alternative name(s): DEAD-box RNA helicase laf-11 Publication |
Gene namesi | ORF Names:Y71H2AM.19Imported |
Organismi | Caenorhabditis elegansImported |
Taxonomic identifieri | 6239 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditina › Rhabditomorpha › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Proteomesi |
|
Organism-specific databases
WormBasei | Y71H2AM.19a ; CE38657 ; WBGene00002244 ; laf-1 Y71H2AM.19b ; CE47305 ; WBGene00002244 ; laf-1 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Plasma membrane
- Cell membrane By similarity
Cytoplasm and Cytosol
- Cytoplasm 2 Publications
- Inflammasome By similarity
Other locations
- Cytoplasmic granule 2 Publications
- Stress granule By similarity
- lamellipodium By similarity
Note: Localizes to P granules in germline precursor cells. Shuttles between the nucleus and the cytosol (By similarity).By similarity1 Publication
Cytosol
- inflammasome complex Source: UniProtKB-SubCell
Nucleus
- nucleus Source: GO_Central
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- cell leading edge Source: UniProtKB
- cytoplasm Source: WormBase
- cytoplasmic stress granule Source: UniProtKB-SubCell
- lamellipodium Source: UniProtKB-SubCell
- P granule Source: WormBase
Keywords - Cellular componenti
Cell membrane, Cell projection, Cytoplasm, Inflammasome, Membrane, NucleusPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 426 | R → C in q80; embryonic lethal. 1 Publication | 1 | |
Mutagenesisi | 430 | M → I in q217; embryonic lethal. 1 Publication | 1 | |
Mutagenesisi | 434 | T → I in q267; embryonic lethal. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000438522 | 1 – 708 | ATP-dependent RNA helicase laf-1CuratedAdd BLAST | 708 |
Proteomic databases
EPDi | D0PV95 |
PeptideAtlasi | D0PV95 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | WBGene00002244, Expressed in material anatomical entity and 4 other tissues |
Interactioni
Subunit structurei
Binds RNA as a monomer at low laf-1 concentrations and as a dimer at high laf-1 concentrations.
1 PublicationGO - Molecular functioni
- identical protein binding Source: UniProtKB
Protein-protein interaction databases
STRINGi | 6239.Y71H2AM.19b |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 262 – 453 | Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 192 | |
Domaini | 465 – 626 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 162 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 191 | DisorderedSequence analysisAdd BLAST | 191 | |
Regioni | 623 – 708 | DisorderedSequence analysisAdd BLAST | 86 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 231 – 259 | Q motifPROSITE-ProRule annotationAdd BLAST | 29 | |
Motifi | 397 – 400 | DEAD boxPROSITE-ProRule annotation | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 72 – 98 | Basic and acidic residuesSequence analysisAdd BLAST | 27 | |
Compositional biasi | 128 – 143 | Polar residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 144 – 167 | Basic and acidic residuesSequence analysisAdd BLAST | 24 | |
Compositional biasi | 168 – 182 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 688 – 708 | Polar residuesSequence analysisAdd BLAST | 21 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0335, Eukaryota |
GeneTreei | ENSGT00940000168275 |
InParanoidi | D0PV95 |
OMAi | IMRGQPV |
OrthoDBi | 595675at2759 |
PhylomeDBi | D0PV95 |
Family and domain databases
CDDi | cd17967, DEADc_DDX3_DDX4, 1 hit |
DisProti | DP01113 |
Gene3Di | 3.40.50.300, 2 hits |
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR044763, Ded1/Dbp1_DEADc IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR000629, RNA-helicase_DEAD-box_CS IPR014014, RNA_helicase_DEAD_Q_motif |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF00271, Helicase_C, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00039, DEAD_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51195, Q_MOTIF, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MESNQSNNGG SGNAALNRGG RYVPPHLRGG DGGAAAAASA GGDDRRGGAG
60 70 80 90 100
GGGYRRGGGN SGGGGGGGYD RGYNDNRDDR DNRGGSGGYG RDRNYEDRGY
110 120 130 140 150
NGGGGGGGNR GYNNNRGGGG GGYNRQDRGD GGSSNFSRGG YNNRDEGSDN
160 170 180 190 200
RGSGRSYNND RRDNGGDGQN TRWNNLDAPP SRGTSKWENR GARDERIEQE
210 220 230 240 250
LFSGQLSGIN FDKYEEIPVE ATGDDVPQPI SLFSDLSLHE WIEENIKTAG
260 270 280 290 300
YDRPTPVQKY SIPALQGGRD LMSCAQTGSG KTAAFLVPLV NAILQDGPDA
310 320 330 340 350
VHRSVTSSGG RKKQYPSALV LSPTRELSLQ IFNESRKFAY RTPITSALLY
360 370 380 390 400
GGRENYKDQI HKLRLGCHIL IATPGRLIDV MDQGLIGMEG CRYLVLDEAD
410 420 430 440 450
RMLDMGFEPQ IRQIVECNRM PSKEERITAM FSATFPKEIQ LLAQDFLKEN
460 470 480 490 500
YVFLAVGRVG STSENIMQKI VWVEEDEKRS YLMDLLDATG DSSLTLVFVE
510 520 530 540 550
TKRGASDLAY YLNRQNYEVV TIHGDLKQFE REKHLDLFRT GTAPILVATA
560 570 580 590 600
VAARGLDIPN VKHVINYDLP SDVDEYVHRI GRTGRVGNVG LATSFFNDKN
610 620 630 640 650
RNIARELMDL IVEANQELPD WLEGMSGDMR SGGGYRGRGG RGNGQRFGGR
660 670 680 690 700
DHRYQGGSGN GGGGNGGGGG FGGGGQRSGG GGGFQSGGGG GRQQQQQQRA
QPQQDWWS
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_058672 | 41 – 105 | Missing in isoform a. CuratedAdd BLAST | 65 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | FJ348231 mRNA Translation: ACO56244.1 BX284603 Genomic DNA Translation: CCD73871.1 BX284603 Genomic DNA Translation: CCG28150.1 |
RefSeqi | NP_001254858.1, NM_001267929.1 NP_001254859.1, NM_001267930.1 [D0PV95-1] |
Genome annotation databases
EnsemblMetazoai | Y71H2AM.19a.1; Y71H2AM.19a.1; WBGene00002244 [D0PV95-2] Y71H2AM.19b.1; Y71H2AM.19b.1; WBGene00002244 [D0PV95-1] |
GeneIDi | 190611 |
KEGGi | cel:CELE_Y71H2AM.19 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | FJ348231 mRNA Translation: ACO56244.1 BX284603 Genomic DNA Translation: CCD73871.1 BX284603 Genomic DNA Translation: CCG28150.1 |
RefSeqi | NP_001254858.1, NM_001267929.1 NP_001254859.1, NM_001267930.1 [D0PV95-1] |
3D structure databases
AlphaFoldDBi | D0PV95 |
SMRi | D0PV95 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 6239.Y71H2AM.19b |
Proteomic databases
EPDi | D0PV95 |
PeptideAtlasi | D0PV95 |
Genome annotation databases
EnsemblMetazoai | Y71H2AM.19a.1; Y71H2AM.19a.1; WBGene00002244 [D0PV95-2] Y71H2AM.19b.1; Y71H2AM.19b.1; WBGene00002244 [D0PV95-1] |
GeneIDi | 190611 |
KEGGi | cel:CELE_Y71H2AM.19 |
Organism-specific databases
CTDi | 190611 |
WormBasei | Y71H2AM.19a ; CE38657 ; WBGene00002244 ; laf-1 Y71H2AM.19b ; CE47305 ; WBGene00002244 ; laf-1 |
Phylogenomic databases
eggNOGi | KOG0335, Eukaryota |
GeneTreei | ENSGT00940000168275 |
InParanoidi | D0PV95 |
OMAi | IMRGQPV |
OrthoDBi | 595675at2759 |
PhylomeDBi | D0PV95 |
Miscellaneous databases
PROi | PR:D0PV95 |
Gene expression databases
Bgeei | WBGene00002244, Expressed in material anatomical entity and 4 other tissues |
Family and domain databases
CDDi | cd17967, DEADc_DDX3_DDX4, 1 hit |
DisProti | DP01113 |
Gene3Di | 3.40.50.300, 2 hits |
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR044763, Ded1/Dbp1_DEADc IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR000629, RNA-helicase_DEAD-box_CS IPR014014, RNA_helicase_DEAD_Q_motif |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF00271, Helicase_C, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00039, DEAD_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51195, Q_MOTIF, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DDX3_CAEEL | |
Accessioni | D0PV95Primary (citable) accession number: D0PV95 Secondary accession number(s): Q4W5R4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 30, 2016 |
Last sequence update: | December 15, 2009 | |
Last modified: | May 25, 2022 | |
This is version 96 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Caenorhabditis annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Caenorhabditis elegans
Caenorhabditis elegans: entries, gene names and cross-references to WormBase - SIMILARITY comments
Index of protein domains and families