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Protein

Thiamine thiazole synthase

Gene

thi4

Organism
Ammonifex degensii (strain DSM 10501 / KC4)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Cofactori

Fe2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei39NADUniRule annotation1
Binding sitei66NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei130NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi158Iron; shared with adjacent protomerUniRule annotation1
Metal bindingi173IronUniRule annotation1
Binding sitei227NAD; via amide nitrogenUniRule annotation1
Binding sitei237GlycineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi58 – 59NADUniRule annotation2
Nucleotide bindingi156 – 158NAD; shared with adjacent protomerUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotation
Biological processThiamine biosynthesisUniRule annotation
LigandIronUniRule annotation, Metal-bindingUniRule annotation, NADUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthaseUniRule annotation (EC:4.-.-.-UniRule annotation)
Gene namesi
Name:thi4UniRule annotation
Ordered Locus Names:Adeg_1609Imported
OrganismiAmmonifex degensii (strain DSM 10501 / KC4)Imported
Taxonomic identifieri429009 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeMoorella groupAmmonifex
Proteomesi
  • UP000002620 Componenti: Chromosome

Interactioni

Subunit structurei

Homooctamer; tetramer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi429009.Adeg_1609

Structurei

3D structure databases

ProteinModelPortaliC9R8S4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105IQJ Bacteria
COG1635 LUCA
HOGENOMiHOG000106048
KOiK22699
OrthoDBiPOG091H007T

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_00304 Thi4, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR002922 Thi4_fam
IPR022828 Thi4_prok
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR00292 TIGR00292, 1 hit

Sequencei

Sequence statusi: Complete.

C9R8S4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGGAIDERL VSRAIIQTYS EELLQLTDFD VAVVGAGPSG LTAAYYLAQG
60 70 80 90 100
GLKTVVFERR LSVGGGMWGG AMMFNYLVFQ EEARPIFETM GVRYREYQPG
110 120 130 140 150
YYVAHSVEAV AAFTLAACRA GARIMNLITV EDLVLRDNRV AGLVLNWTAV
160 170 180 190 200
DMAGMHIDPL AVHCRYVVDA TGHDAEVVRI LTQKNQVTVK VPGGHVQGEK
210 220 230 240 250
SMWSERGEKQ TLDHSGEVFP GLYVAGMAAN AVAGGYRMGP IFGGMVLSGK
260
KVAELILEAH RREKSQTL
Length:268
Mass (Da):29,055
Last modified:November 24, 2009 - v1
Checksum:iE09F98B3D72CEFF2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001785 Genomic DNA Translation: ACX52703.1

Genome annotation databases

EnsemblBacteriaiACX52703; ACX52703; Adeg_1609
KEGGiadg:Adeg_1609

Similar proteinsi

Entry informationi

Entry nameiC9R8S4_AMMDK
AccessioniPrimary (citable) accession number: C9R8S4
Entry historyiIntegrated into UniProtKB/TrEMBL: November 24, 2009
Last sequence update: November 24, 2009
Last modified: July 18, 2018
This is version 56 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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