UniProtKB - C8WMP0 (3AHD_EGGLE)
3alpha-hydroxysteroid dehydrogenase
Elen_0690
Functioni
Involved in the modification of secondary bile acids into iso-bile acids (3beta-bile acids) via epimerization of the 3-OH group through a 3-oxo-intermediate. Catalyzes the oxidation of deoxycholate (DCA) and lithocholate (LCA) to yield 12-alpha-hydroxy-3-oxo-5-beta-cholan-24-oate (3-oxo-DCA) and 3-oxo-5-beta-cholan-24-oate (3-oxo-LCA), respectively. Is also able to catalyze the oxidation of cholate (CA) and chenodeoxycholate (CDCA) into 3-dehydrocholate (3-oxo-CA) and 7-alpha-hydroxy-3-oxo-5-beta-cholan-24-oate (3-oxo-CDCA), respectively. Can also catalyze the reverse reactions in vitro. Prefers NADH to NADPH as cosubstrate. The conversion of the abundant bile acid DCA into isoDCA by the gut bacterium E.lenta favors the growth of the keystone commensal genus Bacteroides, since isoDCA is less cytotoxic than its parent compound, DCA; iso-bile acids have thus a potential role in modulating gut community composition.
1 PublicationCatalytic activityi
- EC:1.1.1.521 PublicationThis reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
Kineticsi
- KM=1280 µM for deoxycholate1 Publication
pH dependencei
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 32 | NADBy similarity | 1 | |
Active sitei | 154 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Binding sitei | 154 | NADBy similarity | 1 | |
Binding sitei | 158 | NADBy similarity | 1 |
GO - Molecular functioni
- 3-alpha-hydroxycholanate dehydrogenase activity Source: UniProtKB-EC
GO - Biological processi
- steroid metabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Lipid metabolism, Steroid metabolism |
Ligand | NAD |
Enzyme and pathway databases
BioCyci | ELEN479437:G1GFY-698-MONOMER |
BRENDAi | 1.1.1.392, 2185 |
Chemistry databases
SwissLipidsi | SLP:000001341 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Ordered Locus Names:Elen_0690Imported |
Organismi | Eggerthella lenta (strain ATCC 25559 / DSM 2243 / CCUG 17323 / JCM 9979 / KCTC 3265 / NCTC 11813 / VPI 0255 / 1899 B) (Eubacterium lentum) |
Taxonomic identifieri | 479437 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Coriobacteriia › Eggerthellales › Eggerthellaceae › Eggerthella › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000443425 | 1 – 259 | 3alpha-hydroxysteroid dehydrogenaseAdd BLAST | 259 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG1028, Bacteria |
HOGENOMi | CLU_010194_1_0_11 |
OMAi | GYANYSS |
OrthoDBi | 1601931at2 |
Family and domain databases
InterProi | View protein in InterPro IPR036291, NAD(P)-bd_dom_sf IPR002347, SDR_fam |
Pfami | View protein in Pfam PF00106, adh_short, 1 hit |
PRINTSi | PR00081, GDHRDH |
SUPFAMi | SSF51735, SSF51735, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MGIYVITGAT SGIGAKTAEI LRERGHEVVN IDLNGGDINA NLATKEGRAG
60 70 80 90 100
AIAELHERFP EGIDAMICNA GVSGGKVPIS LIISLNYFGA TEMARGVFDL
110 120 130 140 150
LEKKGGSCVV TSSNSIAQGA ARMDVAGMLN NHADEDRILE LVKDVDPAIG
160 170 180 190 200
HVYYASTKYA LARWVRRMSP DWGSRGVRLN AIAPGNVRTA MTANMLPEQR
210 220 230 240 250
AAMEAIPVPT HFGEEPLMDP VEIANAMAFI ASPEASGING VVLFVDGGTD
ALLNSEKVY
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP001726 Genomic DNA Translation: ACV54671.1 |
RefSeqi | WP_009306474.1, NC_013204.1 |
Genome annotation databases
EnsemblBacteriai | ACV54671; ACV54671; Elen_0690 |
GeneIDi | 56789104 |
KEGGi | ele:Elen_0690 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP001726 Genomic DNA Translation: ACV54671.1 |
RefSeqi | WP_009306474.1, NC_013204.1 |
3D structure databases
SMRi | C8WMP0 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 479437.Elen_0690 |
Chemistry databases
SwissLipidsi | SLP:000001341 |
Genome annotation databases
EnsemblBacteriai | ACV54671; ACV54671; Elen_0690 |
GeneIDi | 56789104 |
KEGGi | ele:Elen_0690 |
Phylogenomic databases
eggNOGi | COG1028, Bacteria |
HOGENOMi | CLU_010194_1_0_11 |
OMAi | GYANYSS |
OrthoDBi | 1601931at2 |
Enzyme and pathway databases
BioCyci | ELEN479437:G1GFY-698-MONOMER |
BRENDAi | 1.1.1.392, 2185 |
Family and domain databases
InterProi | View protein in InterPro IPR036291, NAD(P)-bd_dom_sf IPR002347, SDR_fam |
Pfami | View protein in Pfam PF00106, adh_short, 1 hit |
PRINTSi | PR00081, GDHRDH |
SUPFAMi | SSF51735, SSF51735, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | 3AHD_EGGLE | |
Accessioni | C8WMP0Primary (citable) accession number: C8WMP0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 28, 2018 |
Last sequence update: | November 3, 2009 | |
Last modified: | February 23, 2022 | |
This is version 62 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families