UniProtKB - C8WGQ3 (3BHD2_EGGLE)
Protein
3beta-hydroxysteroid dehydrogenase 2
Gene
Elen_1325
Organism
Eggerthella lenta (strain ATCC 25559 / DSM 2243 / JCM 9979 / NCTC 11813 / VPI 0255) (Eubacterium lentum)
Status
Functioni
Involved in the modification of secondary bile acids into iso-bile acids (3beta-bile acids) via epimerization of the 3-OH group through a 3-oxo-intermediate. Catalyzes the reduction of 12-alpha-hydroxy-3-oxo-5-beta-cholan-24-oate (3-oxo-DCA) and 3-oxo-5-beta-cholan-24-oate (3-oxo-LCA) to yield isodeoxycholate (isoDCA) and isolithocholate (isoLCA), respectively. Is also able to catalyze the reduction of 3-dehydrocholate (3-oxo-CA or 7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oate) and 7-alpha-hydroxy-3-oxo-5-beta-cholan-24-oate (3-oxo-CDCA), into isocholate (isoCA) and isochenodeoxycholate (isoCDCA), respectively. Accepts both NADH and NADPH as cosubstrates. The conversion of the abundant bile acid deoxycholate (DCA) into isoDCA by the gut bacterium E.lenta favors the growth of the keystone commensal genus Bacteroides, since isoDCA is less cytotoxic than its parent compound, DCA; iso-bile acids have thus a potential role in modulating gut community composition.1 Publication
Catalytic activityi
- EC:1.1.1.3911 PublicationThis reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
Kineticsi
kcat is 1750 min(-1) with 12alpha-hydroxy-3-oxo-5beta-cholan-24-oate as substrate.1 Publication
- KM=2630 µM for 12alpha-hydroxy-3-oxo-5beta-cholan-24-oate1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 43 | NADBy similarity | 1 | |
Binding sitei | 96 | NAD; via carbonyl oxygenBy similarity | 1 | |
Active sitei | 163 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Binding sitei | 163 | NADBy similarity | 1 | |
Binding sitei | 167 | NADBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 69 – 70 | NADBy similarity | 2 |
GO - Molecular functioni
- oxidoreductase activity Source: UniProtKB-KW
GO - Biological processi
- steroid metabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Lipid metabolism, Steroid metabolism |
Ligand | NAD |
Enzyme and pathway databases
BioCyci | ELEN479437:G1GFY-1333-MONOMER |
Chemistry databases
SwissLipidsi | SLP:000001343 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Ordered Locus Names:Elen_1325Imported |
Organismi | Eggerthella lenta (strain ATCC 25559 / DSM 2243 / JCM 9979 / NCTC 11813 / VPI 0255) (Eubacterium lentum) |
Taxonomic identifieri | 479437 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Coriobacteriia › Eggerthellales › Eggerthellaceae › Eggerthella › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000443428 | 1 – 260 | 3beta-hydroxysteroid dehydrogenase 2Add BLAST | 260 |
Family & Domainsi
Sequence similaritiesi
Belongs to the short-chain dehydrogenases/reductases (SDR) family.Curated
Phylogenomic databases
eggNOGi | COG1028, Bacteria |
HOGENOMi | CLU_010194_1_0_11 |
OrthoDBi | 1356861at2 |
Family and domain databases
InterProi | View protein in InterPro IPR036291, NAD(P)-bd_dom_sf IPR020904, Sc_DH/Rdtase_CS IPR002347, SDR_fam |
PRINTSi | PR00081, GDHRDH PR00080, SDRFAMILY |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00061, ADH_SHORT, 1 hit |
i Sequence
Sequence statusi: Complete.
C8WGQ3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSEARHNPVL AGQTAVITGG ASGIGKSIVQ RFLEAGASCL AADLNEEALA
60 70 80 90 100
ALKQELAEYG DKLDVVKVDV SNRDDVEGMV DRAVQTFGQM DIIVNNAGIM
110 120 130 140 150
DNLLPIAEMD DDVWERLMKV NLNSVMYGTR KAVRYFMERG EGGVIINTAS
160 170 180 190 200
LSGLCAGRGG CAYTASKFAV VGLTKNVAFM YADTGIRCNA ICPGNTQTNI
210 220 230 240 250
GVGMRQPSER GMAKATTGYA GATRSGTPEE ISAAAAFLAS DQAGFINGET
260
LTIDGGWSAY
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP001726 Genomic DNA Translation: ACV55294.1 |
RefSeqi | WP_015760525.1, NC_013204.1 |
Genome annotation databases
EnsemblBacteriai | ACV55294; ACV55294; Elen_1325 |
GeneIDi | 40922132 |
KEGGi | ele:Elen_1325 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP001726 Genomic DNA Translation: ACV55294.1 |
RefSeqi | WP_015760525.1, NC_013204.1 |
3D structure databases
SMRi | C8WGQ3 |
ModBasei | Search... |
Chemistry databases
SwissLipidsi | SLP:000001343 |
Genome annotation databases
EnsemblBacteriai | ACV55294; ACV55294; Elen_1325 |
GeneIDi | 40922132 |
KEGGi | ele:Elen_1325 |
Phylogenomic databases
eggNOGi | COG1028, Bacteria |
HOGENOMi | CLU_010194_1_0_11 |
OrthoDBi | 1356861at2 |
Enzyme and pathway databases
BioCyci | ELEN479437:G1GFY-1333-MONOMER |
Family and domain databases
InterProi | View protein in InterPro IPR036291, NAD(P)-bd_dom_sf IPR020904, Sc_DH/Rdtase_CS IPR002347, SDR_fam |
PRINTSi | PR00081, GDHRDH PR00080, SDRFAMILY |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00061, ADH_SHORT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | 3BHD2_EGGLE | |
Accessioni | C8WGQ3Primary (citable) accession number: C8WGQ3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 28, 2018 |
Last sequence update: | November 3, 2009 | |
Last modified: | December 2, 2020 | |
This is version 65 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families