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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

TRM8

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotationSAAS annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei99S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei223S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Active sitei226UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferaseUniRule annotationSAAS annotationImported, RNA-binding, Transferase, tRNA-bindingUniRule annotationSAAS annotation
Biological processtRNA processingUniRule annotationSAAS annotation
LigandS-adenosyl-L-methionineUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
Transfer RNA methyltransferase 8UniRule annotation
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:TRM8UniRule annotation
ORF Names:ANIA_10451Imported
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)Imported
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000000560 Componenti: Chromosome II

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

NucleusUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Forms a complex with TRM82.UniRule annotation

Protein-protein interaction databases

STRINGi162425.CADANIAP00005075

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni122 – 123S-adenosyl-L-methionine bindingUniRule annotation2
Regioni203 – 204S-adenosyl-L-methionine bindingUniRule annotation2
Regioni328 – 330S-adenosyl-L-methionine bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotationSAAS annotation

Phylogenomic databases

HOGENOMiHOG000260965
InParanoidiC8V3V0
OMAiIGMEIRV
OrthoDBiEOG092C3FCG

Family and domain databases

HAMAPiMF_03055 tRNA_methyltr_TrmB_euk, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR025763 Trm8_euk
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
PTHR23417:SF16 PTHR23417:SF16, 1 hit
SUPFAMiSSF53335 SSF53335, 2 hits
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

C8V3V0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGATAKKQKR EVYRNKVAAV TSAESGEAAL AALKLPQKKY YRQRAHANPF
60 70 80 90 100
SDHLLKYPLS PAHMDWATHF PAFVNPDPSQ TNLAGTRKLI KDVEVVDIGC
110 120 130 140 150
GFGGLLVGLA PVLPDTLMVG MEIRVQVTEY LTNRIKALRH QQALKLQLQQ
160 170 180 190 200
SSAASTPAAA PSPSPAPVPE TPTANDPSVD DSEIFPSTLI PGGYQNITAI
210 220 230 240 250
RANTMKFLPN FFARGQLSKI FICFPDPHFK ARKHKARIVS ETLNAEYAYA
260 270 280 290 300
LRPGGLLYTI TDVEEYHYWI LRHFGYDVEA EQAQKQGEGQ EEVQESVGKR
310 320 330 340 350
EGSAELFERV SEEDIEKDEC VRVMKEATEE GKKVARNGGN KYVAVFRRKS

NPEWV
Length:355
Mass (Da):39,484
Last modified:November 3, 2009 - v1
Checksum:i6BD17DA3DDAA77A4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BN001302 Genomic DNA Translation: CBF75671.1

Genome annotation databases

EnsemblFungiiCBF75671; CBF75671; ANIA_10451

Similar proteinsi

Entry informationi

Entry nameiC8V3V0_EMENI
AccessioniPrimary (citable) accession number: C8V3V0
Entry historyiIntegrated into UniProtKB/TrEMBL: November 3, 2009
Last sequence update: November 3, 2009
Last modified: June 20, 2018
This is version 57 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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