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Protein

Thiamine thiazole synthase

Gene

thi4

Organism
Methanocaldococcus fervens (strain DSM 4213 / JCM 15782 / AG86) (Methanococcus fervens)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.UniRule annotation

Cofactori

Fe2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei43NADUniRule annotation1
Binding sitei70NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei134NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi162Iron; shared with adjacent protomerUniRule annotation1
Metal bindingi177IronUniRule annotation1
Binding sitei180NAD; via carbonyl oxygenUniRule annotation1
Binding sitei226NAD; via amide nitrogenUniRule annotation1
Binding sitei236GlycineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi62 – 63NADUniRule annotation2
Nucleotide bindingi160 – 162NAD; shared with adjacent protomerUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotation
Biological processThiamine biosynthesisUniRule annotation
LigandIronUniRule annotation, Metal-bindingUniRule annotation, NADUniRule annotation

Enzyme and pathway databases

BioCyciMFER573064:G1GFN-1419-MONOMER
UniPathwayiUPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthaseUniRule annotation (EC:4.-.-.-UniRule annotation)
Gene namesi
Name:thi4UniRule annotation
Ordered Locus Names:Mefer_1385Imported
OrganismiMethanocaldococcus fervens (strain DSM 4213 / JCM 15782 / AG86) (Methanococcus fervens)Imported
Taxonomic identifieri573064 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000001495 Componenti: Chromosome

Interactioni

Subunit structurei

Homooctamer; tetramer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi573064.Mefer_1385

Family & Domainsi

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00574 Archaea
COG1635 LUCA
HOGENOMiHOG000106048
KOiK22699
OMAiMWGGGMM
OrthoDBiPOG093Z09PU

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_00304 Thi4, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR002922 Thi4_fam
IPR022828 Thi4_prok
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR00292 TIGR00292, 1 hit

Sequencei

Sequence statusi: Complete.

C7P9G0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIKDIKLNA DEVKTAKAIL KASFDMWLDV LEVDVAIVGA GPSGLTCARY
60 70 80 90 100
LAKEGFKVVV LERHLAFGGG TWGGGMGFPY IVIEEPADEL LREVGIKLVY
110 120 130 140 150
AGDGYYVADS VEVPAKLAVA AIDAGAKILT GIVVEDLIVR ENGVSGVVIN
160 170 180 190 200
SYAIEKAGLH IDPLTIKSKI VVDATGHEAS VLNILVKKNK LDLEVPGEKS
210 220 230 240 250
MWAEKGENAL LRNTREVYPN LFVCGMAANA AHGGYRMGAI FGGMYLSGKL
260
CAELIAEKLK NKQ
Length:263
Mass (Da):28,090
Last modified:October 13, 2009 - v1
Checksum:i1A11C9C410DF97D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001696 Genomic DNA Translation: ACV25192.1
RefSeqiWP_015791925.1, NC_013156.1

Genome annotation databases

EnsemblBacteriaiACV25192; ACV25192; Mefer_1385
GeneIDi8366090
KEGGimfe:Mefer_1385

Similar proteinsi

Entry informationi

Entry nameiC7P9G0_METFA
AccessioniPrimary (citable) accession number: C7P9G0
Entry historyiIntegrated into UniProtKB/TrEMBL: October 13, 2009
Last sequence update: October 13, 2009
Last modified: July 18, 2018
This is version 58 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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