Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Endonuclease III

Gene

nth

Organism
Methanocaldococcus fervens (strain DSM 4213 / JCM 15782 / AG86) (Methanococcus fervens)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi184Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi190Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi193Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi201Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-bindingUniRule annotation, GlycosidaseUniRule annotation, Hydrolase, LyaseUniRule annotationImported
Biological processDNA damage, DNA repairUniRule annotation
Ligand4Fe-4SUniRule annotation, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciMFER573064:G1GFN-218-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease IIIUniRule annotation (EC:4.2.99.18UniRule annotation)
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyaseUniRule annotation
Gene namesi
Name:nthUniRule annotation
Ordered Locus Names:Mefer_0216Imported
OrganismiMethanocaldococcus fervens (strain DSM 4213 / JCM 15782 / AG86) (Methanococcus fervens)Imported
Taxonomic identifieri573064 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomada groupMethanococciMethanococcalesMethanocaldococcaceaeMethanocaldococcus
Proteomesi
  • UP000001495 Componenti: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi573064.Mefer_0216

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini229 – 328GIY-YIGInterPro annotationAdd BLAST100

Sequence similaritiesi

Belongs to the Nth/MutY family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00459 Archaea
COG0177 LUCA
COG1833 LUCA
HOGENOMiHOG000254262
KOiK10773
OMAiKLPKKYW
OrthoDBiPOG093Z0AWD

Family and domain databases

CDDicd00056 ENDO3c, 1 hit
cd10441 GIY-YIG_COG1833, 1 hit
Gene3Di1.10.1670.10, 1 hit
HAMAPiMF_00942 Nth, 1 hit
InterProiView protein in InterPro
IPR011257 DNA_glycosylase
IPR002837 DUF123
IPR004036 Endonuclease-III-like_CS2
IPR000305 GIY-YIG_endonuc
IPR003265 HhH-GPD_domain
IPR000445 HhH_motif
IPR023170 HTH_base_excis_C
IPR005759 Nth
PfamiView protein in Pfam
PF01986 DUF123, 1 hit
PF00633 HHH, 1 hit
PF00730 HhH-GPD, 1 hit
SMARTiView protein in SMART
SM00478 ENDO3c, 1 hit
SM00465 GIYc, 1 hit
SUPFAMiSSF48150 SSF48150, 1 hit
PROSITEiView protein in PROSITE
PS01155 ENDONUCLEASE_III_2, 1 hit
PS50164 GIY_YIG, 1 hit

Sequencei

Sequence statusi: Complete.

C7P673-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MELIDILLKK INKNAVVTEI ARDRDPFKVL VSTIISARTK DEVTEEVSKK
60 70 80 90 100
LFKEVKSVDD LLNIEEEKLA DLIYPAGFYR VKAKNLKKLA KILKEKYNGK
110 120 130 140 150
VPDSLEELLK LPGVGRKTAN LVITLAFDKD GICVDTHVHR ICNRWEIVDT
160 170 180 190 200
ETPEETEFEL RKKLPKKYWK VINNLLVVFG KEICSPKPKC NKCFEEIRKK
210 220 230 240 250
CPYYEKIMHF KRILKKFNFR KVSKNNIPNE KGTYILKIRL KEGKKIKFGK
260 270 280 290 300
TERFFKKGYY FYVGSALGNS INLKNRIERH LRENKKMHWH IDYLLKFGKV
310 320 330 340
EEIYITNERV ECDVANEFIK KFDFVENFGC SDCKCKSHLF YLKP
Length:344
Mass (Da):40,576
Last modified:October 13, 2009 - v1
Checksum:iD761A7F9FEE7DEFD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001696 Genomic DNA Translation: ACV24055.1
RefSeqiWP_015790795.1, NC_013156.1

Genome annotation databases

EnsemblBacteriaiACV24055; ACV24055; Mefer_0216
GeneIDi8364881
KEGGimfe:Mefer_0216

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001696 Genomic DNA Translation: ACV24055.1
RefSeqiWP_015790795.1, NC_013156.1

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi573064.Mefer_0216

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACV24055; ACV24055; Mefer_0216
GeneIDi8364881
KEGGimfe:Mefer_0216

Phylogenomic databases

eggNOGiarCOG00459 Archaea
COG0177 LUCA
COG1833 LUCA
HOGENOMiHOG000254262
KOiK10773
OMAiKLPKKYW
OrthoDBiPOG093Z0AWD

Enzyme and pathway databases

BioCyciMFER573064:G1GFN-218-MONOMER

Family and domain databases

CDDicd00056 ENDO3c, 1 hit
cd10441 GIY-YIG_COG1833, 1 hit
Gene3Di1.10.1670.10, 1 hit
HAMAPiMF_00942 Nth, 1 hit
InterProiView protein in InterPro
IPR011257 DNA_glycosylase
IPR002837 DUF123
IPR004036 Endonuclease-III-like_CS2
IPR000305 GIY-YIG_endonuc
IPR003265 HhH-GPD_domain
IPR000445 HhH_motif
IPR023170 HTH_base_excis_C
IPR005759 Nth
PfamiView protein in Pfam
PF01986 DUF123, 1 hit
PF00633 HHH, 1 hit
PF00730 HhH-GPD, 1 hit
SMARTiView protein in SMART
SM00478 ENDO3c, 1 hit
SM00465 GIYc, 1 hit
SUPFAMiSSF48150 SSF48150, 1 hit
PROSITEiView protein in PROSITE
PS01155 ENDONUCLEASE_III_2, 1 hit
PS50164 GIY_YIG, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiC7P673_METFA
AccessioniPrimary (citable) accession number: C7P673
Entry historyiIntegrated into UniProtKB/TrEMBL: October 13, 2009
Last sequence update: October 13, 2009
Last modified: October 10, 2018
This is version 53 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again