UniProtKB - C7NB67 (PAGL_LEPBD)
Protein
6-phospho-alpha-glucosidase
Gene
pagL
Organism
Leptotrichia buccalis (strain ATCC 14201 / DSM 1135 / JCM 12969 / NCTC 10249 / C-1013-b)
Status
Functioni
In vitro, readily hydrolyzes p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate (pNPalphaG6P), a chromogenic analog of the phosphorylated isomers of sucrose. In vivo, is probably involved in the degradation of the 6-phosphate derivatives of the sucrose isomers trehalulose, turanose, maltulose and palatinose, catalyzing their hydrolysis into glucose 6-phosphate (G6P) and fructose, which allows the bacterium to use these sugars as energy sources for growth. Is not able to hydrolyze the C2 or C4 chromogenic stereomers (i.e. pNPalpha-mannopyranoside-6P and pNPalpha-galactopyranoside-6P, respectively).1 Publication
Cofactori
Protein has several cofactor binding sites:- NAD+1 PublicationNote: Binds 1 NAD+ per subunit.1 Publication
- Mn2+1 PublicationNote: Binds 1 Mn2+ ion per subunit.1 Publication
pH dependencei
Optimum pH is 6.9-7.3.1 Publication
: palatinose degradation Pathwayi
This protein is involved in the pathway palatinose degradation, which is part of Glycan degradation.View all proteins of this organism that are known to be involved in the pathway palatinose degradation and in Glycan degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 93 | SubstrateBy similarity | 1 | |
Sitei | 109 | Increases basicity of active site TyrBy similarity | 1 | |
Binding sitei | 147 | SubstrateBy similarity | 1 | |
Metal bindingi | 169 | ManganeseBy similarity | 1 | |
Active sitei | 170 | Proton donorBy similarity | 1 | |
Metal bindingi | 200 | ManganeseBy similarity | 1 | |
Active sitei | 263 | Proton acceptorBy similarity | 1 | |
Binding sitei | 283 | SubstrateBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 4 – 70 | NADBy similarityAdd BLAST | 67 |
GO - Molecular functioni
- hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
- metal ion binding Source: UniProtKB-KW
- oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Source: InterPro
GO - Biological processi
- carbohydrate metabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Biological process | Carbohydrate metabolism |
Ligand | Manganese, Metal-binding, NAD |
Enzyme and pathway databases
BioCyci | LBUC523794:G1GFL-1521-MONOMER |
UniPathwayi | UPA01004 |
Protein family/group databases
CAZyi | GH4, Glycoside Hydrolase Family 4 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:pagL Ordered Locus Names:Lebu_1525 |
Organismi | Leptotrichia buccalis (strain ATCC 14201 / DSM 1135 / JCM 12969 / NCTC 10249 / C-1013-b) |
Taxonomic identifieri | 523794 [NCBI] |
Taxonomic lineagei | Bacteria › Fusobacteria › Fusobacteriales › Leptotrichiaceae › Leptotrichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000415959 | 1 – 440 | 6-phospho-alpha-glucosidaseAdd BLAST | 440 |
Proteomic databases
PRIDEi | C7NB67 |
Expressioni
Inductioni
Induced by growth on the sucrose isomers trehalulose, turanose, maltulose and palatinose.1 Publication
Interactioni
Subunit structurei
Homodimer.
1 PublicationProtein-protein interaction databases
STRINGi | 523794.Lebu_1525 |
Family & Domainsi
Sequence similaritiesi
Belongs to the glycosyl hydrolase 4 family.Curated
Phylogenomic databases
eggNOGi | COG1486, Bacteria |
HOGENOMi | CLU_045951_2_0_0 |
OMAi | METYSPD |
OrthoDBi | 277080at2 |
Family and domain databases
Gene3Di | 3.90.110.10, 1 hit |
InterProi | View protein in InterPro IPR019802, GlycHydrolase_4_CS IPR001088, Glyco_hydro_4 IPR022616, Glyco_hydro_4_C IPR015955, Lactate_DH/Glyco_Ohase_4_C IPR036291, NAD(P)-bd_dom_sf |
PANTHERi | PTHR32092, PTHR32092, 1 hit |
Pfami | View protein in Pfam PF02056, Glyco_hydro_4, 1 hit PF11975, Glyco_hydro_4C, 1 hit |
PRINTSi | PR00732, GLHYDRLASE4 |
SUPFAMi | SSF51735, SSF51735, 1 hit SSF56327, SSF56327, 1 hit |
PROSITEi | View protein in PROSITE PS01324, GLYCOSYL_HYDROL_F4, 1 hit |
i Sequence
Sequence statusi: Complete.
C7NB67-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKKFSIVVAG GGSTFTPGIV LMLLENLDKF PIRQIKFYDN DAQRQEVIAK
60 70 80 90 100
ACDIIIKEKA PDINFVYTTD PETAFTDIDF VMAHIRVGKY AMREKDEKIP
110 120 130 140 150
LRHGVLGQET CGPGGISYGM RSIGGVIELV DYMEKYSPNA WMLNYSNPAA
160 170 180 190 200
IVAEATRRLR PNSKILNICD MPIGIEIRMA EMLGLKSRKD MVIRYFGLNH
210 220 230 240 250
FGWWTDIRDK KGNDLMPALR EKVAKIGYNV EIEGENTEAS WNDTFTKARD
260 270 280 290 300
VFAIDPTTMP NTYLKYYFFP DYVVEHSNPN HTRANEVMEG REKFVFGECR
310 320 330 340 350
AIAEKGTAKD SKLHVDDHAS YIVDLARAIA YDTKERMLLI VENDGAISNF
360 370 380 390 400
DPTAMVEVPC IVGSNGPEKI VQGKIPQFQK GLMEQQVSVE KLTVEAWMEG
410 420 430 440
SYQKLWQAIT LSRTVPSASV AKAILDDLIE ANKDFWPVLK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP001685 Genomic DNA Translation: ACV39398.1 |
RefSeqi | WP_015769739.1, NC_013192.1 |
Genome annotation databases
EnsemblBacteriai | ACV39398; ACV39398; Lebu_1525 |
KEGGi | lba:Lebu_1525 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP001685 Genomic DNA Translation: ACV39398.1 |
RefSeqi | WP_015769739.1, NC_013192.1 |
3D structure databases
SMRi | C7NB67 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 523794.Lebu_1525 |
Protein family/group databases
CAZyi | GH4, Glycoside Hydrolase Family 4 |
Proteomic databases
PRIDEi | C7NB67 |
Genome annotation databases
EnsemblBacteriai | ACV39398; ACV39398; Lebu_1525 |
KEGGi | lba:Lebu_1525 |
Phylogenomic databases
eggNOGi | COG1486, Bacteria |
HOGENOMi | CLU_045951_2_0_0 |
OMAi | METYSPD |
OrthoDBi | 277080at2 |
Enzyme and pathway databases
UniPathwayi | UPA01004 |
BioCyci | LBUC523794:G1GFL-1521-MONOMER |
Family and domain databases
Gene3Di | 3.90.110.10, 1 hit |
InterProi | View protein in InterPro IPR019802, GlycHydrolase_4_CS IPR001088, Glyco_hydro_4 IPR022616, Glyco_hydro_4_C IPR015955, Lactate_DH/Glyco_Ohase_4_C IPR036291, NAD(P)-bd_dom_sf |
PANTHERi | PTHR32092, PTHR32092, 1 hit |
Pfami | View protein in Pfam PF02056, Glyco_hydro_4, 1 hit PF11975, Glyco_hydro_4C, 1 hit |
PRINTSi | PR00732, GLHYDRLASE4 |
SUPFAMi | SSF51735, SSF51735, 1 hit SSF56327, SSF56327, 1 hit |
PROSITEi | View protein in PROSITE PS01324, GLYCOSYL_HYDROL_F4, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PAGL_LEPBD | |
Accessioni | C7NB67Primary (citable) accession number: C7NB67 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 21, 2012 |
Last sequence update: | October 13, 2009 | |
Last modified: | December 2, 2020 | |
This is version 61 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families