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Protein

S-adenosylmethionine synthase

Gene

metK

Organism
Leptotrichia buccalis (strain ATCC 14201 / DSM 1135 / JCM 12969 / NCTC 10249 / C-1013-b)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme.UniRule annotation

Catalytic activityi

ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • K+UniRule annotationNote: Binds 1 potassium ion per subunit.UniRule annotation
  • Mg2+UniRule annotationNote: Binds 2 divalent ions per subunit.UniRule annotation

Pathwayi: S-adenosyl-L-methionine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. S-adenosylmethionine synthase (metK)
This subpathway is part of the pathway S-adenosyl-L-methionine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine, the pathway S-adenosyl-L-methionine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei16ATPUniRule annotation1
Metal bindingi18MagnesiumUniRule annotation1
Metal bindingi44PotassiumUniRule annotation1
Binding sitei57MethionineUniRule annotation1
Binding sitei98MethionineUniRule annotation1
Binding sitei236ATP; shared with neighboring subunitUniRule annotation1
Binding sitei236Methionine; shared with neighboring subunitUniRule annotation1
Binding sitei259ATP; via amide nitrogen; shared with neighboring subunitUniRule annotation1
Binding sitei263ATP; shared with neighboring subunitUniRule annotation1
Binding sitei267MethionineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi158 – 160ATPUniRule annotation3
Nucleotide bindingi227 – 228ATPUniRule annotation2
Nucleotide bindingi242 – 243ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferaseUniRule annotationImported
Biological processOne-carbon metabolismUniRule annotation
LigandATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding, PotassiumUniRule annotation

Enzyme and pathway databases

BioCyciLBUC523794:G1GFL-774-MONOMER
UniPathwayiUPA00315; UER00080

Names & Taxonomyi

Protein namesi
Recommended name:
S-adenosylmethionine synthaseUniRule annotation (EC:2.5.1.6UniRule annotation)
Short name:
AdoMet synthaseUniRule annotation
Alternative name(s):
MATUniRule annotation
Methionine adenosyltransferaseUniRule annotation
Gene namesi
Name:metKUniRule annotation
Ordered Locus Names:Lebu_0761Imported
OrganismiLeptotrichia buccalis (strain ATCC 14201 / DSM 1135 / JCM 12969 / NCTC 10249 / C-1013-b)Imported
Taxonomic identifieri523794 [NCBI]
Taxonomic lineageiBacteriaFusobacteriaFusobacterialesLeptotrichiaceaeLeptotrichia
Proteomesi
  • UP000001910 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homotetramer; dimer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi523794.Lebu_0761

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 100S-AdoMet_synt_NInterPro annotationAdd BLAST96
Domaini110 – 228S-AdoMet_synt_MInterPro annotationAdd BLAST119
Domaini230 – 371S-AdoMet_synt_CInterPro annotationAdd BLAST142

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni98 – 108Flexible loopUniRule annotationAdd BLAST11

Sequence similaritiesi

Belongs to the AdoMet synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CPH Bacteria
COG0192 LUCA
HOGENOMiHOG000245710
KOiK00789
OMAiPGHFLFT
OrthoDBiPOG091H00IO

Family and domain databases

HAMAPiMF_00086 S_AdoMet_synth1, 1 hit
InterProiView protein in InterPro
IPR022631 ADOMET_SYNTHASE_CS
IPR022630 S-AdoMet_synt_C
IPR022629 S-AdoMet_synt_central
IPR022628 S-AdoMet_synt_N
IPR002133 S-AdoMet_synthetase
IPR022636 S-AdoMet_synthetase_sfam
PANTHERiPTHR11964 PTHR11964, 1 hit
PfamiView protein in Pfam
PF02773 S-AdoMet_synt_C, 1 hit
PF02772 S-AdoMet_synt_M, 1 hit
PF00438 S-AdoMet_synt_N, 1 hit
PIRSFiPIRSF000497 MAT, 1 hit
SUPFAMiSSF55973 SSF55973, 3 hits
TIGRFAMsiTIGR01034 metK, 1 hit
PROSITEiView protein in PROSITE
PS00376 ADOMET_SYNTHASE_1, 1 hit
PS00377 ADOMET_SYNTHASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

C7N938-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKKIYFTSE FVSPGHPDKI CDQISDSILD ACLADDENSR VACETFATTG
60 70 80 90 100
LVVVGGEITT KTYVDVQKIV RDKISEIGYR PGMGFDSDCG VLNTIHSQSP
110 120 130 140 150
DISMGVDTGG AGDQGIMFGG AVNETEELMP LALVLSRGII QKLTEITRNG
160 170 180 190 200
TLAWARPDAK AQVTLAYDEN GKLLNVDTVV LSVQHNEDVT NEQIEKDLKE
210 220 230 240 250
LVIKPVLEKY DLNIENVRKF HINPTGRFVI GGPHGDSGLT GRKIIIDTYG
260 270 280 290 300
GYFRHGGGAF SGKDPSKVDR SAAYAARWIA KNIVAAGFAT KCEVQLSYAI
310 320 330 340 350
GVVEPVSIRV ETFGTGTVEE TRIEEAVAKL FDLTPNGIQK SLNLRKPSFR
360 370 380
YQDLAAFGHI GRTDIDLPWE KLDKVEGLKK ELEK
Length:384
Mass (Da):41,928
Last modified:October 13, 2009 - v1
Checksum:iD0ECB722366AF523
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001685 Genomic DNA Translation: ACV38669.1
RefSeqiWP_015769017.1, NC_013192.1

Genome annotation databases

EnsemblBacteriaiACV38669; ACV38669; Lebu_0761
KEGGilba:Lebu_0761

Similar proteinsi

Entry informationi

Entry nameiC7N938_LEPBD
AccessioniPrimary (citable) accession number: C7N938
Entry historyiIntegrated into UniProtKB/TrEMBL: October 13, 2009
Last sequence update: October 13, 2009
Last modified: March 28, 2018
This is version 62 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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