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Protein

Nitrogenase molybdenum-iron protein beta chain

Gene

nifK

Organism
Mesorhizobium caraganae
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation.UniRule annotation

Catalytic activityi

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate.UniRule annotation

Cofactori

[8Fe-7S] clusterUniRule annotationNote: Binds 1 [8Fe-7S] cluster per heterodimer.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductaseUniRule annotation
Biological processNitrogen fixationUniRule annotation
LigandATP-bindingUniRule annotation, Iron, Iron-sulfurUniRule annotation, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogenase molybdenum-iron protein beta chainUniRule annotation (EC:1.18.6.1UniRule annotation)
Alternative name(s):
DinitrogenaseUniRule annotation
Gene namesi
Name:nifKImported
OrganismiMesorhizobium caraganaeImported
Taxonomic identifieri483206 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesPhyllobacteriaceaeMesorhizobium

Subcellular locationi

GO - Cellular componenti

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains. Forms complex with the iron protein (nitrogenase component 2).UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 56DUF3364InterPro annotationAdd BLAST56
Domaini70 – 332Oxidored_nitroInterPro annotationAdd BLAST263

Sequence similaritiesi

Belongs to the NifD/NifK/NifE/NifN family.UniRule annotation

Family and domain databases

InterProiView protein in InterPro
IPR000510 Nase/OxRdtase_comp1
IPR000318 Nase_comp1_CS
IPR005976 Nase_Mo-Fe_CF_bsu
IPR024564 Nase_Mo-Fe_CF_bsu_N
PfamiView protein in Pfam
PF11844 DUF3364, 1 hit
PF00148 Oxidored_nitro, 1 hit
TIGRFAMsiTIGR01286 nifK, 1 hit
PROSITEiView protein in PROSITE
PS00699 NITROGENASE_1_1, 1 hit
PS00090 NITROGENASE_1_2, 1 hit

Sequencei

Sequence statusi: Fragment.

C7DPA1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPQSAEKILD HAPLFREPEY RQMLAEKKLN FECPHPDQIV TDQREFTKTW
60 70 80 90 100
EYREKNLARE ALVVNPAKAC QPLGAVFAAA GFERTMSFVH GSQGCVAYYR
110 120 130 140 150
SHLSRHFKEP ASAVSSSMTE DAAVFGGLKN MVDGLANTYQ LYDPKMIAVS
160 170 180 190 200
TTCMAEVIGD DLHSFIENAK DENSVPRDFD VPFAHTPAFV GSHVDGYDGM
210 220 230 240 250
VKGILEHFWK GQERREAAGT INIIPGFDGF CVGNNRELKR LLDLMSVSYT
260 270 280 290 300
FIQDASDQFD TPSDGEYRMY DGGTKIEDVK KALNAEATLS LQYYNTRKTL
310 320 330
EYCEQSGQET ASFHYPLGVQ ATDEFLMKVS ALPAR
Length:335
Mass (Da):37,537
Last modified:September 22, 2009 - v1
Checksum:i46527F927964527F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei335Imported1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GQ167296 Genomic DNA Translation: ACT67963.1

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GQ167296 Genomic DNA Translation: ACT67963.1

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiView protein in InterPro
IPR000510 Nase/OxRdtase_comp1
IPR000318 Nase_comp1_CS
IPR005976 Nase_Mo-Fe_CF_bsu
IPR024564 Nase_Mo-Fe_CF_bsu_N
PfamiView protein in Pfam
PF11844 DUF3364, 1 hit
PF00148 Oxidored_nitro, 1 hit
TIGRFAMsiTIGR01286 nifK, 1 hit
PROSITEiView protein in PROSITE
PS00699 NITROGENASE_1_1, 1 hit
PS00090 NITROGENASE_1_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiC7DPA1_9RHIZ
AccessioniPrimary (citable) accession number: C7DPA1
Entry historyiIntegrated into UniProtKB/TrEMBL: September 22, 2009
Last sequence update: September 22, 2009
Last modified: July 18, 2018
This is version 21 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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