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Protein

Pyruvate carboxylase

Gene

Amir_5973

Organism
Actinosynnema mirum (strain ATCC 29888 / DSM 43827 / NBRC 14064 / IMRU 3971)
Status
Unreviewed-Annotation score: -Protein predictedi

Functioni

Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.UniRule annotation

Catalytic activityi

ATP + pyruvate + HCO3- = ADP + phosphate + oxaloacetate.UniRule annotation

Cofactori

biotinUniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei117ATPUniRule annotation1
Binding sitei200ATPUniRule annotation1
Binding sitei235ATPUniRule annotation1
Active sitei292UniRule annotation1
Metal bindingi534Divalent metal cationUniRule annotation1
Binding sitei606SubstrateUniRule annotation1
Metal bindingi704Divalent metal cation; via carbamate groupUniRule annotation1
Metal bindingi733Divalent metal cationUniRule annotation1
Metal bindingi735Divalent metal cationUniRule annotation1
Binding sitei868SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotation
LigandATP-bindingUniRule annotation, BiotinUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding, PyruvateImported

Enzyme and pathway databases

BioCyciAMIR446462:G1GEY-6039-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate carboxylaseUniRule annotation (EC:6.4.1.1UniRule annotation)
Gene namesi
Ordered Locus Names:Amir_5973Imported
OrganismiActinosynnema mirum (strain ATCC 29888 / DSM 43827 / NBRC 14064 / IMRU 3971)Imported
Taxonomic identifieri446462 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaPseudonocardialesPseudonocardiaceaeActinosynnema
Proteomesi
  • UP000002213 Componenti: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi446462.Amir_5973

Structurei

3D structure databases

ProteinModelPortaliC6WF06
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 450Biotin carboxylationInterPro annotationAdd BLAST450
Domaini121 – 317ATP-graspInterPro annotationAdd BLAST197
Domaini525 – 794Pyruvate carboxyltransferaseInterPro annotationAdd BLAST270
Domaini1051 – 1125Lipoyl-bindingInterPro annotationAdd BLAST75

Phylogenomic databases

eggNOGiENOG4108JIJ Bacteria
COG1038 LUCA
HOGENOMiHOG000282801
KOiK01958
OMAiEFVDACN
OrthoDBiPOG091H02XZ

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR011761 ATP-grasp
IPR005481 BC-like_N
IPR001882 Biotin_BS
IPR011764 Biotin_carboxylation_dom
IPR005482 Biotin_COase_C
IPR000089 Biotin_lipoyl
IPR003379 Carboxylase_cons_dom
IPR005479 CbamoylP_synth_lsu-like_ATP-bd
IPR016185 PreATP-grasp_dom_sf
IPR000891 PYR_CT
IPR005930 Pyruv_COase
IPR011054 Rudment_hybrid_motif
IPR011053 Single_hybrid_motif
PfamiView protein in Pfam
PF02785 Biotin_carb_C, 1 hit
PF00289 Biotin_carb_N, 1 hit
PF00364 Biotin_lipoyl, 1 hit
PF02786 CPSase_L_D2, 1 hit
PF00682 HMGL-like, 1 hit
PF02436 PYC_OADA, 1 hit
PIRSFiPIRSF001594 Pyruv_carbox, 1 hit
SMARTiView protein in SMART
SM00878 Biotin_carb_C, 1 hit
SUPFAMiSSF51230 SSF51230, 1 hit
SSF51246 SSF51246, 1 hit
SSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01235 pyruv_carbox, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS50979 BC, 1 hit
PS00188 BIOTIN, 1 hit
PS50968 BIOTINYL_LIPOYL, 1 hit
PS00867 CPSASE_2, 1 hit
PS50991 PYR_CT, 1 hit

Sequencei

Sequence statusi: Complete.

C6WF06-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFRKVLVANR GEIAIRAFRA AYELGAGTVA VFPHEDRNSL HRLKADESYE
60 70 80 90 100
IGEPGHPVRA YLSVDEVIKA ARKAGADAVY PGYGFLSENP GLAKACADAG
110 120 130 140 150
ITFVGPPTEV LELTGNKASA IAAARAAGLP VLKSSDPSSD VDALLAAADD
160 170 180 190 200
IGFPVFVKAV AGGGGRGMRR VEDPAALRES IEAASREAES AFGDPTVFLE
210 220 230 240 250
QAVVEPRHIE VQILADGQGG VIHLFERDCS VQRRHQKVIE IAPAPNLSPE
260 270 280 290 300
LRERICGDAV RFAEHIGYRN AGTVEFLLDP RGNYVFIEMN PRIQVEHTVT
310 320 330 340 350
EEVTDVDLVQ SQMRIASGET LADLGLSQDT VQLRGAALQC RITTEDPANG
360 370 380 390 400
FRPDTGMISA YRSPGGSGVR LDGGTTGVGT AISAHFDSML VKLTCRGRTF
410 420 430 440 450
SAAVARARRA VAEFRIRGVS TNIPFLQAVL DDPDFYEGRV TTSFIEKRPH
460 470 480 490 500
LLTARSSADR GTRLLTYLAD VTVNRPNGAR PSVVDPRLKL PPVDVSSDPA
510 520 530 540 550
PGSKQRLDQL GPEGFARWLR SSERVGVTDT TFRDAHQSLL ATRVRTKDLL
560 570 580 590 600
AVAPHVARLT PELLSLEAWG GATYDVALRF LAEDPWERLA ALREAVPNIA
610 620 630 640 650
LQMLLRGRNT VGYTPYPEAV TSAFVEEATA TGIDIFRIFD ALNDVEQMRP
660 670 680 690 700
AIEAVRATGT AVAEVALCYT ADLSNPDERL YTLDYYLRLA EQIVSAGAHV
710 720 730 740 750
LAVKDMAGLL RPPAAAKLIT ALRSEFDLPV HLHTHDTAGG QLATYLAAIQ
760 770 780 790 800
SGVDAVDGAA ASMGGTTSQP PLSAIVAATD HTPHATGLDL AAVCDLEPYW
810 820 830 840 850
ESVRKVYAPF ESGIPGPTGR VYSHEIPGGQ LSNLRTQAVA LGLGQKFEEI
860 870 880 890 900
EAMYAAADRM LGRLVKVTPS SKVVGDLALH LVGAGVSPAE FEAEPGRFDV
910 920 930 940 950
PGSVIGFLHG ELGDPPGGWP EPFRSKALKG RAAPKAVAEL TEDDRKGLAE
960 970 980 990 1000
KPRATLNRLL FPAPTKEYLA HRDAYGDTSV LSSKDFFYGL RPGEEYPVDL
1010 1020 1030 1040 1050
EPGVRLLIRL EAIGEADERG VRTVMAVLNG QLRPIQVRDR SVAAEVPAAE
1060 1070 1080 1090 1100
KADRANPNHV AAPFSGVVQP SVAEGDEVDA GQTLATIEAM KMEAAITAPK
1110 1120
AGRVARLAVG NVQQVEGGDL LVVLE
Length:1,125
Mass (Da):120,300
Last modified:September 22, 2009 - v1
Checksum:i0A22F8247E574113
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001630 Genomic DNA Translation: ACU39781.1
RefSeqiWP_015804666.1, NC_013093.1

Genome annotation databases

EnsemblBacteriaiACU39781; ACU39781; Amir_5973
KEGGiami:Amir_5973

Similar proteinsi

Entry informationi

Entry nameiC6WF06_ACTMD
AccessioniPrimary (citable) accession number: C6WF06
Entry historyiIntegrated into UniProtKB/TrEMBL: September 22, 2009
Last sequence update: September 22, 2009
Last modified: April 25, 2018
This is version 61 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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