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Protein

Endonuclease III homolog

Gene

NTH1

Organism
Plasmodium falciparum (isolate 3D7)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage. The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site. The AP lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines.UniRule annotation

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster does not appear to play a role in catalysis, but is probably involved in the proper positioning of the enzyme along the DNA strand.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei335Nucleophile; for N-glycosylase activityUniRule annotation1
Sitei354Important for catalytic activityUniRule annotation1
Metal bindingi403Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi410Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi413Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi419Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonucleaseImported, GlycosidaseUniRule annotation, Hydrolase, LyaseUniRule annotationImported, Nuclease
Biological processDNA damage, DNA repairUniRule annotation
Ligand4Fe-4SUniRule annotation, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

ReactomeiR-PFA-110329 Cleavage of the damaged pyrimidine
R-PFA-110357 Displacement of DNA glycosylase by APEX1

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease III homologUniRule annotation (EC:3.2.2.-UniRule annotation, EC:4.2.99.18UniRule annotation)
Alternative name(s):
Bifunctional DNA N-glycosylase/DNA-(apurinic or apyrimidinic site) lyaseUniRule annotation
Short name:
DNA glycosylase/AP lyaseUniRule annotation
Gene namesi
Name:NTH1UniRule annotation
ORF Names:PF3D7_0614800Imported
OrganismiPlasmodium falciparum (isolate 3D7)Imported
Taxonomic identifieri36329 [NCBI]
Taxonomic lineageiEukaryotaAlveolataApicomplexaAconoidasidaHaemosporidaPlasmodiidaePlasmodiumPlasmodium (Laverania)
Proteomesi
  • UP000001450 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiPlasmoDB:PF3D7_0614800

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

MitochondrionUniRule annotation, NucleusUniRule annotation

Structurei

3D structure databases

ProteinModelPortaliC6KSY9
SMRiC6KSY9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini253 – 401ENDO3cInterPro annotationAdd BLAST149
Domaini324 – 343HhH1InterPro annotationAdd BLAST20

Sequence similaritiesi

Belongs to the Nth/MutY family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000281511
InParanoidiC6KSY9
KOiK10773
OMAiCESCKIN
PhylomeDBiC6KSY9

Family and domain databases

CDDicd00056 ENDO3c, 1 hit
Gene3Di1.10.1670.10, 1 hit
HAMAPiMF_03183 Endonuclease_III_Nth, 1 hit
InterProiView protein in InterPro
IPR011257 DNA_glycosylase
IPR004036 Endonuclease-III-like_CS2
IPR003651 Endonuclease3_FeS-loop_motif
IPR004035 Endouclease-III_FeS-bd_BS
IPR003265 HhH-GPD_domain
IPR000445 HhH_motif
IPR003583 Hlx-hairpin-Hlx_DNA-bd_motif
IPR023170 HTH_base_excis_C
IPR030841 NTH1
PfamiView protein in Pfam
PF00633 HHH, 1 hit
PF00730 HhH-GPD, 1 hit
SMARTiView protein in SMART
SM00478 ENDO3c, 1 hit
SM00525 FES, 1 hit
SM00278 HhH1, 1 hit
SUPFAMiSSF48150 SSF48150, 1 hit
PROSITEiView protein in PROSITE
PS00764 ENDONUCLEASE_III_1, 1 hit
PS01155 ENDONUCLEASE_III_2, 1 hit

Sequencei

Sequence statusi: Complete.

C6KSY9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKLFPYLNKI CLLNLYFIKM EKPSIYFMNN KIKKIQIQYE ERENNAPMKN
60 70 80 90 100
FMLDEKKENY DITYKINIKN EQTSYPSNDD IKSHCQDKPD EGNVYKNINL
110 120 130 140 150
TKKGNKKEHM NDSLLNKRKV KTEYVIKQKQ MKREKEQTVL EEGIKHVHEL
160 170 180 190 200
KKRKNKNVKS DKIKIDEKGV DKKFIDEKGV DKKFIDEKGV DKKIIDEKNV
210 220 230 240 250
EFKRAHFLLT YNKLKEMRKD IIAPVDKYGC DKLSEKTNDM KVFRFQTLIS
260 270 280 290 300
CMLSSRTKDE VTAMVMDKLK KHGLTVHNIL NTTEEQLKKL IYGIGFYNVK
310 320 330 340 350
AKQILQICHI LKNKYNSDIP HTYEELKKLP GIGEKIAQLI LQTALNKHEG
360 370 380 390 400
IAVDIHVHRI ANRLNWVNSK NELDTQMKLK SYVQKELWSE INHVLVGFGQ
410 420 430
VICKGKKPLC EKCTLTNKCQ YYQDTSTKQE NKSNEYI
Length:437
Mass (Da):51,539
Last modified:September 1, 2009 - v1
Checksum:i9564704B39C1CB22
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL844505 Genomic DNA Translation: CAG25386.2
RefSeqiXP_966134.2, XM_961041.2

Genome annotation databases

EnsemblProtistsiCAG25386; CAG25386; PF3D7_0614800
GeneDBiPF3D7_0614800.1:pep
GeneIDi3885808
KEGGipfa:PF3D7_0614800

Similar proteinsi

Entry informationi

Entry nameiC6KSY9_PLAF7
AccessioniPrimary (citable) accession number: C6KSY9
Entry historyiIntegrated into UniProtKB/TrEMBL: September 1, 2009
Last sequence update: September 1, 2009
Last modified: September 12, 2018
This is version 69 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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