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Protein

Thiazole synthase

Gene

thiG

Organism
Pectobacterium carotovorum subsp. carotovorum (strain PC1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H2S.UniRule annotation

Catalytic activityi

1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O.UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei95Schiff-base intermediate with DXPUniRule annotation1
Binding sitei156DXP; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processThiamine biosynthesis
LigandSchiff base

Enzyme and pathway databases

BioCyciPCAR561230:G1GF8-217-MONOMER
UniPathwayi
UPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
Thiazole synthaseUniRule annotation (EC:2.8.1.10UniRule annotation)
Gene namesi
Name:thiGUniRule annotation
Ordered Locus Names:PC1_0212
OrganismiPectobacterium carotovorum subsp. carotovorum (strain PC1)
Taxonomic identifieri561230 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesPectobacteriaceaePectobacterium
Proteomesi
  • UP000002736 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002061391 – 261Thiazole synthaseAdd BLAST261

Proteomic databases

PRIDEiC6DHS2

Interactioni

Subunit structurei

Homotetramer. Forms heterodimers with either ThiH or ThiS.UniRule annotation

Protein-protein interaction databases

STRINGi561230.PC1_0212

Structurei

3D structure databases

ProteinModelPortaliC6DHS2
SMRiC6DHS2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni182 – 183DXP bindingUniRule annotation2
Regioni204 – 205DXP bindingUniRule annotation2

Sequence similaritiesi

Belongs to the ThiG family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CA8 Bacteria
COG2022 LUCA
HOGENOMiHOG000248049
KOiK03149
OMAiAQYPSPA
OrthoDBiPOG091H01K9

Family and domain databases

CDDicd04728 ThiG, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00443 ThiG, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR033983 Thiazole_synthase_ThiG
IPR008867 ThiG
PANTHERiPTHR34266 PTHR34266, 1 hit
PfamiView protein in Pfam
PF05690 ThiG, 1 hit

Sequencei

Sequence statusi: Complete.

C6DHS2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLHIADTTLT SRLLTGTGKF ATPELMLAAL EASGSQLVTM AMKRVDLNGG
60 70 80 90 100
NDAILAPLRQ LGIKLLPNTS GAKTADEAIF AARLAREALG THWLKLEIHP
110 120 130 140 150
DVKYLLPDPI ETLKAAEQLV KEGFTVLPYC GADPVLCKRL EEVGCAAVMP
160 170 180 190 200
LGAPIGSNQG LQTRDFLRII IEQARVPVIV DAGIGAPSQA ADALEMGADA
210 220 230 240 250
VLVNTAIAVA RDPVAMARAF RLAVEAGGLA RQAGLGSKQF VASATSPLTG
260
FLHQQAEGAV R
Length:261
Mass (Da):27,320
Last modified:September 1, 2009 - v1
Checksum:i00D888388E4EDBA4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001657 Genomic DNA Translation: ACT11272.1
RefSeqiWP_012772942.1, NC_012917.1

Genome annotation databases

EnsemblBacteriaiACT11272; ACT11272; PC1_0212
GeneIDi29703003
KEGGipct:PC1_0212

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001657 Genomic DNA Translation: ACT11272.1
RefSeqiWP_012772942.1, NC_012917.1

3D structure databases

ProteinModelPortaliC6DHS2
SMRiC6DHS2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi561230.PC1_0212

Proteomic databases

PRIDEiC6DHS2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACT11272; ACT11272; PC1_0212
GeneIDi29703003
KEGGipct:PC1_0212

Phylogenomic databases

eggNOGiENOG4105CA8 Bacteria
COG2022 LUCA
HOGENOMiHOG000248049
KOiK03149
OMAiAQYPSPA
OrthoDBiPOG091H01K9

Enzyme and pathway databases

UniPathwayi
UPA00060

BioCyciPCAR561230:G1GF8-217-MONOMER

Family and domain databases

CDDicd04728 ThiG, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00443 ThiG, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR033983 Thiazole_synthase_ThiG
IPR008867 ThiG
PANTHERiPTHR34266 PTHR34266, 1 hit
PfamiView protein in Pfam
PF05690 ThiG, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTHIG_PECCP
AccessioniPrimary (citable) accession number: C6DHS2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: September 1, 2009
Last modified: October 10, 2018
This is version 54 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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