pckG

Functionⁱ

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityⁱ

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO₂.UniRule annotation

Cofactorⁱ

Mn²⁺UniRule annotationNote: Binds 1 Mn²⁺ ion per subunit.UniRule annotation

Pathwayⁱ: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	86	SubstrateUniRule annotation	1
Metal bindingⁱ	229	ManganeseUniRule annotation	1
Metal bindingⁱ	248	Manganese; via tele nitrogenUniRule annotation	1
Binding siteⁱ	270	SubstrateUniRule annotation	1
Active siteⁱ	272	UniRule annotation	1
Metal bindingⁱ	289	ManganeseUniRule annotation	1
Binding siteⁱ	386	GTPUniRule annotation	1
Binding siteⁱ	418	GTPUniRule annotation	1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	271 – 276	GTPUniRule annotation		6

GO - Molecular functionⁱ

GTP binding Source: UniProtKB-KW
metal ion binding Source: UniProtKB-KW
phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-EC

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

gluconeogenesis Source: UniProtKB-UniPathway

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Decarboxylase, Lyase
Biological process	Gluconeogenesis
Ligand	GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCycⁱ	TSIB604354:G1GV3-150-MONOMER
UniPathwayⁱ	UPA00138

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation) Short name: PEP carboxykinaseUniRule annotation Short name: PEPCKUniRule annotation
Gene namesⁱ	Name:pckGUniRule annotation Ordered Locus Names:TSIB_0151
Organismⁱ	Thermococcus sibiricus (strain MM 739 / DSM 12597)
Taxonomic identifierⁱ	604354 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus › Thermococcus sibiricus
Proteomesⁱ	UP000009079 Componentⁱ: Chromosome

Subcellular locationⁱ

Cytoplasm
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_00452

GO - Cellular componentⁱ

cytoplasm Source: UniProtKB-SubCell

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cytoplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_1000206234	1 – 622	Phosphoenolpyruvate carboxykinase [GTP]Add BLAST		622

Proteomic databases

PRIDEⁱ	C6A0S4

PRIDEⁱ

C6A0S4

Interactionⁱ

Protein-protein interaction databases

STRINGⁱ	604354.TSIB_0151

STRINGⁱ

604354.TSIB_0151

Structureⁱ

3D structure databases

SMRⁱ	C6A0S4
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	220 – 222	Substrate bindingUniRule annotation		3
Regionⁱ	384 – 386	Substrate bindingUniRule annotation		3

Sequence similaritiesⁱ

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGⁱ	arCOG05865 Archaea COG1274 LUCA
HOGENOMⁱ	HOG000191700
KEGG Orthology (KO) More...KOⁱ	K01596
OMAⁱ	CWLALTD
Database of Orthologous Groups More...OrthoDBⁱ	POG093Z01SN

Family and domain databases

Gene3Dⁱ	3.40.449.10, 2 hits 3.90.228.20, 2 hits
HAMAPⁱ	MF_00452 PEPCK_GTP, 1 hit
InterProⁱ	View protein in InterPro IPR018091 PEP_carboxykin_GTP_CS IPR013035 PEP_carboxykinase_C IPR008209 PEP_carboxykinase_GTP IPR035077 PEP_carboxykinase_GTP_C IPR035078 PEP_carboxykinase_GTP_N IPR008210 PEP_carboxykinase_N
PANTHERⁱ	PTHR11561 PTHR11561, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00821 PEPCK_C, 1 hit PF17297 PEPCK_N, 1 hit
PIRSFⁱ	PIRSF001348 PEP_carboxykinase_GTP, 1 hit
PROSITEⁱ	View protein in PROSITE PS00505 PEPCK_GTP, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

C6A0S4-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MTPEDYLKKR LDPEQFEKIK GIDNPELNEF LAKYIELLNP ARVFICTDSK 
        60         70         80         90        100
EDENYIRRRA IEYGEEKSLA MEGHTIHYDG YYDQARDKAR TKILVPKGVE 
       110        120        130        140        150
IPFINTMDRE KGLKEIHEIM KDIAKGKELF VCFFVLGPKN SVFTIPAVQL 
       160        170        180        190        200
TDSAYVAHSE FILYRKGYEE FKRLGREAKF LKFVHSAGEL DERKTSKNID 
       210        220        230        240        250
KRRVYIDLEG ETVYSANTQY GGNTIGLKKL AFRLTIKRAV EEGWLSEHMF 
       260        270        280        290        300
LMRINGPNGR KTYFTGAYPS MCGKTSTAMI SWENIVGDDL TFIVDMKGEA 
       310        320        330        340        350
RGANVEKGVF GIIQGVNQED DPIIWEVLHS PNEIIFSNVL VKDGKPYWNE 
       360        370        380        390        400
MGIPIPDEGE NHSGKWWRGK KDSEGNEISP SHKNARFTVS LDAFPNTDLE 
       410        420        430        440        450
ALETPCGVRV GGMIFGGRDA DTWPPVREAF DWAHGVITMG AALESETTAA 
       460        470        480        490        500
TLGKEGVRAF NPMAILDFLS VHIGKYLKNY LEFEKKLRIK PKIFAVNYFL 
       510        520        530        540        550
REKDGKWLNH KLDKSVWLKW MELRVHGDVD AIKTPVGYIP KYEDLKRLFK 
       560        570        580        590        600
EVLNKDYSRE DYEKQFVIRV PEFLAKIERI EKIYRDVGNI PEELFKVLEE 
       610        620 
ERHRLIEAKE KYGDYISPFK FL

Length:622

Mass (Da):71,790

Last modified:September 1, 2009 - v1

Checksum:ⁱ68A4AFFA3E5792FE

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CP001463 Genomic DNA Translation: ACS89219.1
NCBI Reference Sequences More...RefSeqⁱ	WP_012766180.1, NC_012883.1

Genome annotation databases

EnsemblBacteriaⁱ	ACS89219; ACS89219; TSIB_0151
GeneIDⁱ	8095123
KEGGⁱ	tsi:TSIB_0151

Protein	Similar proteins		Species	Score	Length
C6A0S4	Phosphoenolpyruvate carboxykinase [GTP]		Thermococcus sp. 40_45		622
Phosphoenolpyruvate carboxykinase [GTP]		Thermococcus sibiricus		622

Protein	Similar proteins		Species	Score	Length
C6A0S4	Phosphoenolpyruvate carboxykinase [GTP]		Thermococcus sp. 40_45		622
Phosphoenolpyruvate carboxykinase [GTP]		Thermococcus sibiricus		622
Phosphoenolpyruvate carboxykinase [GTP]		THELN		624
Phosphoenolpyruvate carboxykinase [GTP]		Thermococcales archaeon 44_46		624
Phosphoenolpyruvate carboxykinase [GTP]		Thermococcus sp. 2319x1		624
+4

Protein	Similar proteins		Species	Score	Length
C6A0S4	Phosphoenolpyruvate carboxykinase [GTP]		Thermococcus sp. 40_45		622
Phosphoenolpyruvate carboxykinase [GTP]		Thermococcus sibiricus		622
UPI0005B2AEDF		Thermococcus sp. PK		624
Phosphoenolpyruvate carboxykinase [GTP]		Thermococcus sp. 2319x1		624
Phosphoenolpyruvate carboxykinase [GTP]		Thermococcales archaeon 44_46		624
+39

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	CP001463 Genomic DNA Translation: ACS89219.1
NCBI Reference Sequences More...RefSeqⁱ	WP_012766180.1, NC_012883.1

3D structure databases

SMRⁱ	C6A0S4
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	604354.TSIB_0151

STRINGⁱ

604354.TSIB_0151

Proteomic databases

PRIDEⁱ	C6A0S4

PRIDEⁱ

C6A0S4

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	ACS89219; ACS89219; TSIB_0151
GeneIDⁱ	8095123
KEGGⁱ	tsi:TSIB_0151

Phylogenomic databases

eggNOGⁱ	arCOG05865 Archaea COG1274 LUCA
HOGENOMⁱ	HOG000191700
KEGG Orthology (KO) More...KOⁱ	K01596
OMAⁱ	CWLALTD
Database of Orthologous Groups More...OrthoDBⁱ	POG093Z01SN

Enzyme and pathway databases

UniPathwayⁱ	UPA00138
BioCycⁱ	TSIB604354:G1GV3-150-MONOMER

Family and domain databases

Gene3Dⁱ	3.40.449.10, 2 hits 3.90.228.20, 2 hits
HAMAPⁱ	MF_00452 PEPCK_GTP, 1 hit
InterProⁱ	View protein in InterPro IPR018091 PEP_carboxykin_GTP_CS IPR013035 PEP_carboxykinase_C IPR008209 PEP_carboxykinase_GTP IPR035077 PEP_carboxykinase_GTP_C IPR035078 PEP_carboxykinase_GTP_N IPR008210 PEP_carboxykinase_N
PANTHERⁱ	PTHR11561 PTHR11561, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00821 PEPCK_C, 1 hit PF17297 PEPCK_N, 1 hit
PIRSFⁱ	PIRSF001348 PEP_carboxykinase_GTP, 1 hit
PROSITEⁱ	View protein in PROSITE PS00505 PEPCK_GTP, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	PCKG_THESM
Accessionⁱ	C6A0S4Primary (citable) accession number: C6A0S4
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
	Last sequence update:	September 1, 2009
	Last modified:	May 23, 2018
	This is version 57 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

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Supporting data

UniProtKB - C6A0S4 (PCKG_THESM)

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Phosphoenolpyruvate carboxykinase [GTP]

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: gluconeogenesis

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

Pathwayⁱ: gluconeogenesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ