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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Thermococcus sibiricus (strain MM 739 / DSM 12597)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei86SubstrateUniRule annotation1
Metal bindingi229ManganeseUniRule annotation1
Metal bindingi248Manganese; via tele nitrogenUniRule annotation1
Binding sitei270SubstrateUniRule annotation1
Active sitei272UniRule annotation1
Metal bindingi289ManganeseUniRule annotation1
Binding sitei386GTPUniRule annotation1
Binding sitei418GTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi271 – 276GTPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylase, Lyase
Biological processGluconeogenesis
LigandGTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTSIB604354:G1GV3-150-MONOMER
UniPathwayiUPA00138

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:TSIB_0151
OrganismiThermococcus sibiricus (strain MM 739 / DSM 12597)
Taxonomic identifieri604354 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
Proteomesi
  • UP000009079 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002062341 – 622Phosphoenolpyruvate carboxykinase [GTP]Add BLAST622

Proteomic databases

PRIDEiC6A0S4

Interactioni

Protein-protein interaction databases

STRINGi604354.TSIB_0151

Structurei

3D structure databases

SMRiC6A0S4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni220 – 222Substrate bindingUniRule annotation3
Regioni384 – 386Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG05865 Archaea
COG1274 LUCA
HOGENOMiHOG000191700
KOiK01596
OMAiCWLALTD
OrthoDBiPOG093Z01SN

Family and domain databases

Gene3Di3.40.449.10, 2 hits
3.90.228.20, 2 hits
HAMAPiMF_00452 PEPCK_GTP, 1 hit
InterProiView protein in InterPro
IPR018091 PEP_carboxykin_GTP_CS
IPR013035 PEP_carboxykinase_C
IPR008209 PEP_carboxykinase_GTP
IPR035077 PEP_carboxykinase_GTP_C
IPR035078 PEP_carboxykinase_GTP_N
IPR008210 PEP_carboxykinase_N
PANTHERiPTHR11561 PTHR11561, 1 hit
PfamiView protein in Pfam
PF00821 PEPCK_C, 1 hit
PF17297 PEPCK_N, 1 hit
PIRSFiPIRSF001348 PEP_carboxykinase_GTP, 1 hit
PROSITEiView protein in PROSITE
PS00505 PEPCK_GTP, 1 hit

Sequencei

Sequence statusi: Complete.

C6A0S4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPEDYLKKR LDPEQFEKIK GIDNPELNEF LAKYIELLNP ARVFICTDSK
60 70 80 90 100
EDENYIRRRA IEYGEEKSLA MEGHTIHYDG YYDQARDKAR TKILVPKGVE
110 120 130 140 150
IPFINTMDRE KGLKEIHEIM KDIAKGKELF VCFFVLGPKN SVFTIPAVQL
160 170 180 190 200
TDSAYVAHSE FILYRKGYEE FKRLGREAKF LKFVHSAGEL DERKTSKNID
210 220 230 240 250
KRRVYIDLEG ETVYSANTQY GGNTIGLKKL AFRLTIKRAV EEGWLSEHMF
260 270 280 290 300
LMRINGPNGR KTYFTGAYPS MCGKTSTAMI SWENIVGDDL TFIVDMKGEA
310 320 330 340 350
RGANVEKGVF GIIQGVNQED DPIIWEVLHS PNEIIFSNVL VKDGKPYWNE
360 370 380 390 400
MGIPIPDEGE NHSGKWWRGK KDSEGNEISP SHKNARFTVS LDAFPNTDLE
410 420 430 440 450
ALETPCGVRV GGMIFGGRDA DTWPPVREAF DWAHGVITMG AALESETTAA
460 470 480 490 500
TLGKEGVRAF NPMAILDFLS VHIGKYLKNY LEFEKKLRIK PKIFAVNYFL
510 520 530 540 550
REKDGKWLNH KLDKSVWLKW MELRVHGDVD AIKTPVGYIP KYEDLKRLFK
560 570 580 590 600
EVLNKDYSRE DYEKQFVIRV PEFLAKIERI EKIYRDVGNI PEELFKVLEE
610 620
ERHRLIEAKE KYGDYISPFK FL
Length:622
Mass (Da):71,790
Last modified:September 1, 2009 - v1
Checksum:i68A4AFFA3E5792FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001463 Genomic DNA Translation: ACS89219.1
RefSeqiWP_012766180.1, NC_012883.1

Genome annotation databases

EnsemblBacteriaiACS89219; ACS89219; TSIB_0151
GeneIDi8095123
KEGGitsi:TSIB_0151

Similar proteinsi

Entry informationi

Entry nameiPCKG_THESM
AccessioniPrimary (citable) accession number: C6A0S4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: September 1, 2009
Last modified: May 23, 2018
This is version 57 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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