UniProtKB - C6A0S4 (PCKG_THESM)
Protein
Phosphoenolpyruvate carboxykinase [GTP]
Gene
pckG
Organism
Thermococcus sibiricus (strain DSM 12597 / MM 739)
Status
Functioni
Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation
Catalytic activityi
- EC:4.1.1.32UniRule annotation
Cofactori
Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation
: gluconeogenesis Pathwayi
This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotationView all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 86 | SubstrateUniRule annotation | 1 | |
Metal bindingi | 229 | ManganeseUniRule annotation | 1 | |
Metal bindingi | 248 | Manganese; via tele nitrogenUniRule annotation | 1 | |
Binding sitei | 270 | SubstrateUniRule annotation | 1 | |
Active sitei | 272 | UniRule annotation | 1 | |
Metal bindingi | 289 | ManganeseUniRule annotation | 1 | |
Binding sitei | 386 | GTPUniRule annotation | 1 | |
Binding sitei | 418 | GTPUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 271 – 276 | GTPUniRule annotation | 6 |
GO - Molecular functioni
- GTP binding Source: UniProtKB-UniRule
- manganese ion binding Source: UniProtKB-UniRule
- phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-UniRule
GO - Biological processi
- gluconeogenesis Source: UniProtKB-UniRule
Keywordsi
Molecular function | Decarboxylase, Lyase |
Biological process | Gluconeogenesis |
Ligand | GTP-binding, Manganese, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | TSIB604354:G1GV3-150-MONOMER |
UniPathwayi | UPA00138 |
Names & Taxonomyi
Protein namesi | Recommended name: Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)Short name: PEP carboxykinaseUniRule annotation Short name: PEPCKUniRule annotation |
Gene namesi | Name:pckGUniRule annotation Ordered Locus Names:TSIB_0151 |
Organismi | Thermococcus sibiricus (strain DSM 12597 / MM 739) |
Taxonomic identifieri | 604354 [NCBI] |
Taxonomic lineagei | Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Thermococcus › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_1000206234 | 1 – 622 | Phosphoenolpyruvate carboxykinase [GTP]Add BLAST | 622 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 220 – 222 | Substrate bindingUniRule annotation | 3 | |
Regioni | 384 – 386 | Substrate bindingUniRule annotation | 3 |
Sequence similaritiesi
Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation
Phylogenomic databases
eggNOGi | arCOG05865, Archaea |
HOGENOMi | CLU_028872_1_1_2 |
OMAi | GPTNNWV |
OrthoDBi | 37571at2157 |
Family and domain databases
Gene3Di | 3.40.449.10, 1 hit 3.90.228.20, 1 hit |
HAMAPi | MF_00452, PEPCK_GTP, 1 hit |
InterProi | View protein in InterPro IPR018091, PEP_carboxykin_GTP_CS IPR013035, PEP_carboxykinase_C IPR008209, PEP_carboxykinase_GTP IPR035077, PEP_carboxykinase_GTP_C IPR035078, PEP_carboxykinase_GTP_N IPR008210, PEP_carboxykinase_N |
PANTHERi | PTHR11561, PTHR11561, 1 hit |
Pfami | View protein in Pfam PF00821, PEPCK_GTP, 1 hit PF17297, PEPCK_N, 1 hit |
PIRSFi | PIRSF001348, PEP_carboxykinase_GTP, 1 hit |
SUPFAMi | SSF68923, SSF68923, 1 hit |
PROSITEi | View protein in PROSITE PS00505, PEPCK_GTP, 1 hit |
i Sequence
Sequence statusi: Complete.
C6A0S4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTPEDYLKKR LDPEQFEKIK GIDNPELNEF LAKYIELLNP ARVFICTDSK
60 70 80 90 100
EDENYIRRRA IEYGEEKSLA MEGHTIHYDG YYDQARDKAR TKILVPKGVE
110 120 130 140 150
IPFINTMDRE KGLKEIHEIM KDIAKGKELF VCFFVLGPKN SVFTIPAVQL
160 170 180 190 200
TDSAYVAHSE FILYRKGYEE FKRLGREAKF LKFVHSAGEL DERKTSKNID
210 220 230 240 250
KRRVYIDLEG ETVYSANTQY GGNTIGLKKL AFRLTIKRAV EEGWLSEHMF
260 270 280 290 300
LMRINGPNGR KTYFTGAYPS MCGKTSTAMI SWENIVGDDL TFIVDMKGEA
310 320 330 340 350
RGANVEKGVF GIIQGVNQED DPIIWEVLHS PNEIIFSNVL VKDGKPYWNE
360 370 380 390 400
MGIPIPDEGE NHSGKWWRGK KDSEGNEISP SHKNARFTVS LDAFPNTDLE
410 420 430 440 450
ALETPCGVRV GGMIFGGRDA DTWPPVREAF DWAHGVITMG AALESETTAA
460 470 480 490 500
TLGKEGVRAF NPMAILDFLS VHIGKYLKNY LEFEKKLRIK PKIFAVNYFL
510 520 530 540 550
REKDGKWLNH KLDKSVWLKW MELRVHGDVD AIKTPVGYIP KYEDLKRLFK
560 570 580 590 600
EVLNKDYSRE DYEKQFVIRV PEFLAKIERI EKIYRDVGNI PEELFKVLEE
610 620
ERHRLIEAKE KYGDYISPFK FL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP001463 Genomic DNA Translation: ACS89219.1 |
RefSeqi | WP_012766180.1, NC_012883.1 |
Genome annotation databases
EnsemblBacteriai | ACS89219; ACS89219; TSIB_0151 |
GeneIDi | 8095123 |
KEGGi | tsi:TSIB_0151 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP001463 Genomic DNA Translation: ACS89219.1 |
RefSeqi | WP_012766180.1, NC_012883.1 |
3D structure databases
SMRi | C6A0S4 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 604354.TSIB_0151 |
Genome annotation databases
EnsemblBacteriai | ACS89219; ACS89219; TSIB_0151 |
GeneIDi | 8095123 |
KEGGi | tsi:TSIB_0151 |
Phylogenomic databases
eggNOGi | arCOG05865, Archaea |
HOGENOMi | CLU_028872_1_1_2 |
OMAi | GPTNNWV |
OrthoDBi | 37571at2157 |
Enzyme and pathway databases
UniPathwayi | UPA00138 |
BioCyci | TSIB604354:G1GV3-150-MONOMER |
Family and domain databases
Gene3Di | 3.40.449.10, 1 hit 3.90.228.20, 1 hit |
HAMAPi | MF_00452, PEPCK_GTP, 1 hit |
InterProi | View protein in InterPro IPR018091, PEP_carboxykin_GTP_CS IPR013035, PEP_carboxykinase_C IPR008209, PEP_carboxykinase_GTP IPR035077, PEP_carboxykinase_GTP_C IPR035078, PEP_carboxykinase_GTP_N IPR008210, PEP_carboxykinase_N |
PANTHERi | PTHR11561, PTHR11561, 1 hit |
Pfami | View protein in Pfam PF00821, PEPCK_GTP, 1 hit PF17297, PEPCK_N, 1 hit |
PIRSFi | PIRSF001348, PEP_carboxykinase_GTP, 1 hit |
SUPFAMi | SSF68923, SSF68923, 1 hit |
PROSITEi | View protein in PROSITE PS00505, PEPCK_GTP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PCKG_THESM | |
Accessioni | C6A0S4Primary (citable) accession number: C6A0S4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 22, 2009 |
Last sequence update: | September 1, 2009 | |
Last modified: | December 2, 2020 | |
This is version 69 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families