Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Biotin synthase

Gene

bioB

Organism
Neisseria flavescens SK114
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotationSAAS annotation

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotationSAAS annotation

Pathwayi: biotin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.UniRule annotationSAAS annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Biotin synthase (bioB)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi108Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi112Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi115Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi152Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi183Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi243Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi315Iron-sulfur 2 (2Fe-2S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferaseUniRule annotationSAAS annotationImported
Biological processBiotin biosynthesisUniRule annotationSAAS annotation
Ligand2Fe-2SUniRule annotationSAAS annotation, 4Fe-4SUniRule annotationSAAS annotation, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionineUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayi
UPA00078;UER00162

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotationSAAS annotation (EC:2.8.1.6UniRule annotationSAAS annotation)
Gene namesi
Name:bioBUniRule annotationImported
ORF Names:NEIFL0001_1190Imported
OrganismiNeisseria flavescens SK114Imported
Taxonomic identifieri596320 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria
Proteomesi
  • UP000005533 Componenti: Unassembled WGS sequence

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi596320.NEIFL0001_1190

Structurei

3D structure databases

ProteinModelPortaliC5TMU2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini98 – 304Elp3InterPro annotationAdd BLAST207
Domaini275 – 367BATSInterPro annotationAdd BLAST93

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105CZF Bacteria
COG0502 LUCA
OrthoDBiPOG091H01DF

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01694 BioB, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR010722 BATS_dom
IPR034416 BATS_domain_containing
IPR002684 Biotin_synth/BioAB
IPR024177 Biotin_synthase
IPR006638 Elp3/MiaB/NifB
IPR007197 rSAM
PANTHERiPTHR22976 PTHR22976, 1 hit
PfamiView protein in Pfam
PF06968 BATS, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF001619 Biotin_synth, 1 hit
SFLDiSFLDF00272 biotin_synthase, 1 hit
SFLDG01060 BATS_domain_containing, 1 hit
SFLDG01278 biotin_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00876 BATS, 1 hit
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00433 bioB, 1 hit

Sequencei

Sequence statusi: Complete.

C5TMU2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLRRFRRPFI LISNLLLPSL RRQSSRQRHC VAVNTKEHTM TVSPVALRRQ
60 70 80 90 100
TDRKPHPTAR YWKKCDVEAL FGLPFLDLVF QAAEIHRQNF NPREIQLSTL
110 120 130 140 150
LSIKTGGCPE DCAYCPQSAH HNTNLGKEQM MDVDEIVEKA KIAQSRGASR
160 170 180 190 200
FCMGAAWRGP KPKDVAVVSE IISAVKGLGM EVCGTFGMLE DGMAEDFKKA
210 220 230 240 250
GLDYYNHNLD TDPERYNDII HTRKHEDRMD TLGKVRNAGL KVCCGGIVGM
260 270 280 290 300
NETRAERAGL IASLANLDPQ PESVPINQLV KVEGTPLADA EDLDWTEFVR
310 320 330 340 350
TIAVARITMP HSYVRLSAGR SNMPESMQAM CFMAGANSIF YGDKLLTTGN
360 370 380
PDEDGDRLLM EKLNLYPLRF ELEEEYKAAQ ETPKIKVDY
Length:389
Mass (Da):43,763
Last modified:September 1, 2009 - v1
Checksum:i3D928A5D46052B61
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
ACQV01000024 Genomic DNA Translation: EER55871.1

Genome annotation databases

EnsemblBacteriaiEER55871; EER55871; NEIFL0001_1190

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
ACQV01000024 Genomic DNA Translation: EER55871.1

3D structure databases

ProteinModelPortaliC5TMU2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi596320.NEIFL0001_1190

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiEER55871; EER55871; NEIFL0001_1190

Phylogenomic databases

eggNOGiENOG4105CZF Bacteria
COG0502 LUCA
OrthoDBiPOG091H01DF

Enzyme and pathway databases

UniPathwayi
UPA00078;UER00162

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01694 BioB, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR010722 BATS_dom
IPR034416 BATS_domain_containing
IPR002684 Biotin_synth/BioAB
IPR024177 Biotin_synthase
IPR006638 Elp3/MiaB/NifB
IPR007197 rSAM
PANTHERiPTHR22976 PTHR22976, 1 hit
PfamiView protein in Pfam
PF06968 BATS, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF001619 Biotin_synth, 1 hit
SFLDiSFLDF00272 biotin_synthase, 1 hit
SFLDG01060 BATS_domain_containing, 1 hit
SFLDG01278 biotin_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00876 BATS, 1 hit
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00433 bioB, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiC5TMU2_NEIFL
AccessioniPrimary (citable) accession number: C5TMU2
Entry historyiIntegrated into UniProtKB/TrEMBL: September 1, 2009
Last sequence update: September 1, 2009
Last modified: November 22, 2017
This is version 45 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again