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Protein

Thiamine thiazole synthase

Gene

THI4

Organism
Zygosaccharomyces rouxii (strain ATCC 2623 / CBS 732 / NBRC 1130 / NCYC 568 / NRRL Y-229) (Candida mogii)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance.UniRule annotation

Cofactori

Fe cationUniRule annotationNote: Binds 1 Fe cation per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei76Substrate; via amide nitrogenUniRule annotation1
Binding sitei105Substrate; via amide nitrogenUniRule annotation1
Binding sitei170Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei207SubstrateUniRule annotation1
Binding sitei237SubstrateUniRule annotation1
Binding sitei291Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processThiamine biosynthesisUniRule annotation
LigandIronUniRule annotation, Metal-bindingUniRule annotation, NADUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthaseUniRule annotation
Alternative name(s):
Thiazole biosynthetic enzymeUniRule annotation
Gene namesi
Name:THI4UniRule annotation
Ordered Locus Names:ZYRO0G11726gImported
OrganismiZygosaccharomyces rouxii (strain ATCC 2623 / CBS 732 / NBRC 1130 / NCYC 568 / NRRL Y-229) (Candida mogii)Imported
Taxonomic identifieri559307 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeZygosaccharomyces
Proteomesi
  • UP000008536 Componenti: Chromosome G

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

CytoplasmUniRule annotation, NucleusUniRule annotation

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2052,3-didehydroalanine (Cys)UniRule annotation1

Post-translational modificationi

During the catalytic reaction, a sulfide is transferred from Cys-205 to a reaction intermediate, generating a dehydroalanine residue.UniRule annotation

Interactioni

Subunit structurei

Homooctamer.UniRule annotation

Protein-protein interaction databases

STRINGi4956.XP_002498498.1

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni97 – 98Substrate bindingUniRule annotation2
Regioni301 – 303Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000106048
InParanoidiC5E0D1
KOiK03146
OrthoDBiEOG092C3CAV

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_03158 THI4, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR027495 Sti35
IPR002922 Thi4_fam
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR00292 TIGR00292, 1 hit

Sequencei

Sequence statusi: Complete.

C5E0D1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSATATQTQE VTQLQLNAQQ PIRHALSDVV KTEDWSDFQF TPIRESTVSR
60 70 80 90 100
AMTRRYFQDL DKHAVSDVVI IGAGSSGLSA AYVICKNRPD LTVTCVEANV
110 120 130 140 150
SPGGGAWLGG QLFSAMIMRK PAHLFLDELE VPYDDEGDYV VVKHAALFTS
160 170 180 190 200
TVLSKVLQFP NFKLFNATAV EDLVTRPAGP NGEVTVAGVV TNWTLVTMAH
210 220 230 240 250
DLQSCMDPNV IELAGYKNDG TRDAEKKHGV ILSTTGHDGP FGAFSAKRIV
260 270 280 290 300
DIDSTNKLKG MKGLDMNHAE ADVVKNSGSY NNVGSVYFAG MEVSELSGCN
310 320
RMGPTFGAMA VSGIKAAEEI LRHFA
Length:325
Mass (Da):35,052
Last modified:July 28, 2009 - v1
Checksum:i9C173ED75846548A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928179 Genomic DNA Translation: CAR29565.1
RefSeqiXP_002498498.1, XM_002498453.1

Genome annotation databases

EnsemblFungiiCAR29565; CAR29565; ZYRO0G11726g
GeneIDi8206309
KEGGizro:ZYRO0G11726g

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928179 Genomic DNA Translation: CAR29565.1
RefSeqiXP_002498498.1, XM_002498453.1

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4956.XP_002498498.1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAR29565; CAR29565; ZYRO0G11726g
GeneIDi8206309
KEGGizro:ZYRO0G11726g

Phylogenomic databases

HOGENOMiHOG000106048
InParanoidiC5E0D1
KOiK03146
OrthoDBiEOG092C3CAV

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_03158 THI4, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR027495 Sti35
IPR002922 Thi4_fam
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR00292 TIGR00292, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiC5E0D1_ZYGRC
AccessioniPrimary (citable) accession number: C5E0D1
Entry historyiIntegrated into UniProtKB/TrEMBL: July 28, 2009
Last sequence update: July 28, 2009
Last modified: November 7, 2018
This is version 51 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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