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Protein

Thiamine thiazole synthase

Gene

THI4

Organism
Lachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284) (Yeast) (Kluyveromyces thermotolerans)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance.UniRule annotation

Cofactori

Fe cationUniRule annotationNote: Binds 1 Fe cation per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei74Substrate; via amide nitrogenUniRule annotation1
Binding sitei103Substrate; via amide nitrogenUniRule annotation1
Binding sitei168Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei206SubstrateUniRule annotation1
Binding sitei236SubstrateUniRule annotation1
Binding sitei290Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processThiamine biosynthesisUniRule annotation
LigandIronUniRule annotation, Metal-bindingUniRule annotation, NADUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthaseUniRule annotation
Alternative name(s):
Thiazole biosynthetic enzymeUniRule annotation
Gene namesi
Name:THI4UniRule annotation
Ordered Locus Names:KLTH0B03916gImported
OrganismiLachancea thermotolerans (strain ATCC 56472 / CBS 6340 / NRRL Y-8284) (Yeast) (Kluyveromyces thermotolerans)Imported
Taxonomic identifieri559295 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeLachancea
Proteomesi
  • UP000002036 Componenti: Chromosome B

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

CytoplasmUniRule annotation, NucleusUniRule annotation

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2042,3-didehydroalanine (Cys)UniRule annotation1

Post-translational modificationi

During the catalytic reaction, a sulfide is transferred from Cys-204 to a reaction intermediate, generating a dehydroalanine residue.UniRule annotation

Interactioni

Subunit structurei

Homooctamer.UniRule annotation

Protein-protein interaction databases

STRINGi381046.XP_002551959.1

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni95 – 96Substrate bindingUniRule annotation2
Regioni300 – 302Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000106048
InParanoidiC5DCL0
KOiK03146
OrthoDBiEOG092C3CAV

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_03158 THI4, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR027495 Sti35
IPR002922 Thi4_fam
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR00292 TIGR00292, 1 hit

Sequencei

Sequence statusi: Complete.

C5DCL0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSTTTTNTET AQLQVSAGTI RHALSDVVKQ EDWSDFQFAP IRESTVSRAM
60 70 80 90 100
TSRYFQDMDK FAVSDVVIVG AGSSGLSAAY VIAKNRPDLK VACVESNVAP
110 120 130 140 150
GGGAWLGGQL FSAMIMRKPA HLFLDELELP YEDEGDYVVV KHAALFTSTV
160 170 180 190 200
LSKVLLFPNF KLFNATAVED LVTRPADDNT GGVSVAGVVT NWTLVSMAHD
210 220 230 240 250
LQSCMDPNVI ELSGYQNDGT RDPSKKHGVI LSTTGHDGPF GAFSAKRIVS
260 270 280 290 300
IDKNKKLGGM KGLDMNRAEA GVVKGAGAYS GVANMYFAGM EVAELEGCNR
310 320
MGPTFGAMAV SGIKAAEEIL RHFAE
Length:325
Mass (Da):34,687
Last modified:July 28, 2009 - v1
Checksum:i72DD18F8EA6DDDA3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928166 Genomic DNA Translation: CAR21521.1
RefSeqiXP_002551959.1, XM_002551913.1

Genome annotation databases

EnsemblFungiiCAR21521; CAR21521; KLTH0B03916g
GeneIDi8290793
KEGGilth:KLTH0B03916g

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928166 Genomic DNA Translation: CAR21521.1
RefSeqiXP_002551959.1, XM_002551913.1

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi381046.XP_002551959.1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAR21521; CAR21521; KLTH0B03916g
GeneIDi8290793
KEGGilth:KLTH0B03916g

Phylogenomic databases

HOGENOMiHOG000106048
InParanoidiC5DCL0
KOiK03146
OrthoDBiEOG092C3CAV

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_03158 THI4, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR027495 Sti35
IPR002922 Thi4_fam
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR00292 TIGR00292, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiC5DCL0_LACTC
AccessioniPrimary (citable) accession number: C5DCL0
Entry historyiIntegrated into UniProtKB/TrEMBL: July 28, 2009
Last sequence update: July 28, 2009
Last modified: October 25, 2017
This is version 48 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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