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Protein

Pyridoxal 5'-phosphate synthase subunit PdxT

Gene

pdxT

Organism
Kosmotoga olearia (strain TBF 19.5.1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PdxS.UniRule annotation

Catalytic activityi

D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate.UniRule annotation
L-glutamine + H2O = L-glutamate + NH3.UniRule annotation

Pathwayi: pyridoxal 5'-phosphate biosynthesis

This protein is involved in the pathway pyridoxal 5'-phosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway pyridoxal 5'-phosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei78NucleophileUniRule annotation1
Binding sitei106L-glutamineUniRule annotation1
Active sitei171Charge relay systemUniRule annotation1
Active sitei173Charge relay systemUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Lyase
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciKOLE521045:G1GF2-812-MONOMER
UniPathwayiUPA00245

Names & Taxonomyi

Protein namesi
Recommended name:
Pyridoxal 5'-phosphate synthase subunit PdxTUniRule annotation (EC:4.3.3.6UniRule annotation)
Alternative name(s):
Pdx2UniRule annotation
Pyridoxal 5'-phosphate synthase glutaminase subunitUniRule annotation (EC:3.5.1.2UniRule annotation)
Gene namesi
Name:pdxTUniRule annotation
Ordered Locus Names:Kole_0803
OrganismiKosmotoga olearia (strain TBF 19.5.1)
Taxonomic identifieri521045 [NCBI]
Taxonomic lineageiBacteriaThermotogaeKosmotogalesKosmotogaceaeKosmotoga
Proteomesi
  • UP000002382 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002157161 – 192Pyridoxal 5'-phosphate synthase subunit PdxTAdd BLAST192

Interactioni

Subunit structurei

In the presence of PdxS, forms a dodecamer of heterodimers. Only shows activity in the heterodimer.UniRule annotation

Protein-protein interaction databases

STRINGi521045.Kole_0803

Structurei

3D structure databases

ProteinModelPortaliC5CG73
SMRiC5CG73
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni46 – 48L-glutamine bindingUniRule annotation3
Regioni135 – 136L-glutamine bindingUniRule annotation2

Sequence similaritiesi

Belongs to the glutaminase PdxT/SNO family.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4108UHX Bacteria
COG0311 LUCA
HOGENOMiHOG000039949
KOiK08681
OMAiVFIRAPI
OrthoDBiPOG091H084H

Family and domain databases

CDDicd01749 GATase1_PB, 1 hit
Gene3Di3.40.50.880, 1 hit
HAMAPiMF_01615 PdxT, 1 hit
InterProiView protein in InterPro
IPR029062 Class_I_gatase-like
IPR002161 PdxT/SNO
PANTHERiPTHR31559 PTHR31559, 1 hit
PfamiView protein in Pfam
PF01174 SNO, 1 hit
PIRSFiPIRSF005639 Glut_amidoT_SNO, 1 hit
SUPFAMiSSF52317 SSF52317, 1 hit
TIGRFAMsiTIGR03800 PLP_synth_Pdx2, 1 hit
PROSITEiView protein in PROSITE
PS51130 PDXT_SNO_2, 1 hit

Sequencei

Sequence statusi: Complete.

C5CG73-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIGVLGIQG DIQEHLRMIE KTGNEPLWVK STSELAEVSG LIMPGGESTT
60 70 80 90 100
MIRLMKKYEL WDALREAIAS GLPVYATCAG MILLAREIIN YPEQETLGVL
110 120 130 140 150
DIAVERNGYG RQVASFETDL EIPAIGDTPF RAIFIRAPII EKCGDSVEVL
160 170 180 190
STYKEKPVLV KQGKILASSF HPELTDDLRI HEYFVKEIIG KE
Length:192
Mass (Da):21,378
Last modified:July 28, 2009 - v1
Checksum:iAA8950038D528C44
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001634 Genomic DNA Translation: ACR79514.1
RefSeqiWP_015868176.1, NC_012785.1

Genome annotation databases

EnsemblBacteriaiACR79514; ACR79514; Kole_0803
KEGGikol:Kole_0803

Similar proteinsi

Entry informationi

Entry nameiPDXT_KOSOT
AccessioniPrimary (citable) accession number: C5CG73
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: February 28, 2018
This is version 46 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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