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Protein

Glutamate 5-kinase

Gene

proB

Organism
Kosmotoga olearia (strain TBF 19.5.1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB)
  2. Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei7ATPUniRule annotation1
Binding sitei47SubstrateUniRule annotation1
Binding sitei135SubstrateUniRule annotation1
Binding sitei147Substrate; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi202 – 208ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAmino-acid biosynthesis, Proline biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciKOLE521045:G1GF2-1428-MONOMER
UniPathwayiUPA00098; UER00359

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:Kole_1419
OrganismiKosmotoga olearia (strain TBF 19.5.1)
Taxonomic identifieri521045 [NCBI]
Taxonomic lineageiBacteriaThermotogaeKosmotogalesKosmotogaceaeKosmotoga
Proteomesi
  • UP000002382 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002062721 – 359Glutamate 5-kinaseAdd BLAST359

Interactioni

Protein-protein interaction databases

STRINGi521045.Kole_1419

Structurei

3D structure databases

ProteinModelPortaliC5CE10
SMRiC5CE10
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini266 – 343PUAUniRule annotationAdd BLAST78

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CGT Bacteria
COG0263 LUCA
HOGENOMiHOG000246369
KOiK00931
OMAiTHEIRFG
OrthoDBiPOG091H00OF

Family and domain databases

Gene3Di2.30.130.10, 1 hit
3.40.1160.10, 1 hit
HAMAPiMF_00456 ProB, 1 hit
InterProiView protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR001048 Asp/Glu/Uridylate_kinase
IPR001057 Glu/AcGlu_kinase
IPR011529 Glu_5kinase
IPR005715 Glu_5kinase/COase_Synthase
IPR019797 Glutamate_5-kinase_CS
IPR002478 PUA
IPR015947 PUA-like_sf
IPR036974 PUA_sf
PfamiView protein in Pfam
PF00696 AA_kinase, 1 hit
PF01472 PUA, 1 hit
PIRSFiPIRSF000729 GK, 1 hit
PRINTSiPR00474 GLU5KINASE
SMARTiView protein in SMART
SM00359 PUA, 1 hit
SUPFAMiSSF53633 SSF53633, 1 hit
SSF88697 SSF88697, 1 hit
TIGRFAMsiTIGR01027 proB, 1 hit
PROSITEiView protein in PROSITE
PS00902 GLUTAMATE_5_KINASE, 1 hit
PS50890 PUA, 1 hit

Sequencei

Sequence statusi: Complete.

C5CE10-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKITIKVGS NLLVQQDGQL DKRYIVELCR EIGNLMSEGH QVVLVSSGAR
60 70 80 90 100
AAGYGYLNKS NAQEADLYMK QALCAVGQVQ LMKLYESAMS FYGIKVAQIL
110 120 130 140 150
LTREDFSHRK RFLNLRNTLI GLTEMGILPI VNENDSVATE EIMFGDNDVL
160 170 180 190 200
ASMFAIGWNA DYLLLMTSVD GVIDQNGKVI PFYREDTTNM AIAKNASSRW
210 220 230 240 250
GSGGITSKIR AARAAAAAGI QTSICNGKKL ENIAQFVLTG QTGTVFERVG
260 270 280 290 300
PIKAKKAWIG FLSKPKGSIF INEGAKIAIE NNRSLLPVGV VHIEGDFQAG
310 320 330 340 350
DVVEIRLDDG TFLGKGIINF SSAEAKKIVG LKSDQLEDVL GYSCSKVLIH

IDNLWKRDN
Length:359
Mass (Da):39,174
Last modified:July 28, 2009 - v1
Checksum:i43137309B2A700A7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001634 Genomic DNA Translation: ACR80112.1
RefSeqiWP_015868759.1, NC_012785.1

Genome annotation databases

EnsemblBacteriaiACR80112; ACR80112; Kole_1419
KEGGikol:Kole_1419

Similar proteinsi

Entry informationi

Entry nameiPROB_KOSOT
AccessioniPrimary (citable) accession number: C5CE10
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 28, 2009
Last modified: February 28, 2018
This is version 53 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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