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Protein

S-adenosylmethionine synthase

Gene

metK

Organism
Micrococcus luteus (strain ATCC 4698 / DSM 20030 / JCM 1464 / NBRC 3333 / NCIMB 9278 / NCTC 2665 / VKM Ac-2230) (Micrococcus lysodeikticus)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme.UniRule annotation

Catalytic activityi

ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • K+UniRule annotationNote: Binds 1 potassium ion per subunit.UniRule annotation
  • Mg2+UniRule annotationNote: Binds 2 divalent ions per subunit.UniRule annotation

Pathwayi: S-adenosyl-L-methionine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. S-adenosylmethionine synthase (metK)
This subpathway is part of the pathway S-adenosyl-L-methionine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine, the pathway S-adenosyl-L-methionine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36ATPUniRule annotation1
Metal bindingi38MagnesiumUniRule annotation1
Metal bindingi64PotassiumUniRule annotation1
Binding sitei77MethionineUniRule annotation1
Binding sitei120MethionineUniRule annotation1
Binding sitei274ATP; shared with neighboring subunitUniRule annotation1
Binding sitei274Methionine; shared with neighboring subunitUniRule annotation1
Binding sitei297ATP; via amide nitrogen; shared with neighboring subunitUniRule annotation1
Binding sitei301ATP; shared with neighboring subunitUniRule annotation1
Binding sitei305MethionineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi197 – 199ATPUniRule annotation3
Nucleotide bindingi265 – 266ATPUniRule annotation2
Nucleotide bindingi280 – 281ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferaseUniRule annotationImported
Biological processOne-carbon metabolismUniRule annotation
LigandATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding, PotassiumUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00315; UER00080

Names & Taxonomyi

Protein namesi
Recommended name:
S-adenosylmethionine synthaseUniRule annotation (EC:2.5.1.6UniRule annotation)
Short name:
AdoMet synthaseUniRule annotation
Alternative name(s):
MATUniRule annotation
Methionine adenosyltransferaseUniRule annotation
Gene namesi
Name:metKUniRule annotation
Ordered Locus Names:Mlut_12520Imported
OrganismiMicrococcus luteus (strain ATCC 4698 / DSM 20030 / JCM 1464 / NBRC 3333 / NCIMB 9278 / NCTC 2665 / VKM Ac-2230) (Micrococcus lysodeikticus)Imported
Taxonomic identifieri465515 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicrococcalesMicrococcaceaeMicrococcus
Proteomesi
  • UP000000738 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

PTM / Processingi

Proteomic databases

PRIDEiC5CCE5

Interactioni

Subunit structurei

Homotetramer; dimer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi465515.MlutN2_010100000862

Structurei

3D structure databases

ProteinModelPortaliC5CCE5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 121S-AdoMet_synt_NInterPro annotationAdd BLAST96
Domaini145 – 266S-AdoMet_synt_MInterPro annotationAdd BLAST122
Domaini268 – 407S-AdoMet_synt_CInterPro annotationAdd BLAST140

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni120 – 130Flexible loopUniRule annotationAdd BLAST11

Sequence similaritiesi

Belongs to the AdoMet synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CPH Bacteria
COG0192 LUCA
HOGENOMiHOG000245710
KOiK00789
OMAiPGHFLFT
OrthoDBiPOG091H00IO

Family and domain databases

HAMAPiMF_00086 S_AdoMet_synth1, 1 hit
InterProiView protein in InterPro
IPR022631 ADOMET_SYNTHASE_CS
IPR022630 S-AdoMet_synt_C
IPR022629 S-AdoMet_synt_central
IPR022628 S-AdoMet_synt_N
IPR002133 S-AdoMet_synthetase
IPR022636 S-AdoMet_synthetase_sfam
PANTHERiPTHR11964 PTHR11964, 1 hit
PfamiView protein in Pfam
PF02773 S-AdoMet_synt_C, 1 hit
PF02772 S-AdoMet_synt_M, 1 hit
PF00438 S-AdoMet_synt_N, 1 hit
PIRSFiPIRSF000497 MAT, 1 hit
SUPFAMiSSF55973 SSF55973, 3 hits
TIGRFAMsiTIGR01034 metK, 1 hit
PROSITEiView protein in PROSITE
PS00376 ADOMET_SYNTHASE_1, 1 hit
PS00377 ADOMET_SYNTHASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

C5CCE5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDAASLSPA RHADPYSGVG GGELRLFTSE SVTAGHPDKI CDQISDAILD
60 70 80 90 100
AILAADPSAK VAVETLTTTG LVQVAGEVNT SAYVEIPKIV RETILDIGYD
110 120 130 140 150
SSANGFDGAQ CGVNISIGQQ SSDIFAGVSR SLEAREYGSA DEVDEQGAGD
160 170 180 190 200
QGIMFGYATD ETPAYMPMPI YLAHRLSERL TAVRRAENTP MPYLLPDGKT
210 220 230 240 250
QVTIGYAPGH VPTTVEAVVV STQHHEWVTQ EQLRADVEEH VIRPVIERSG
260 270 280 290 300
LDTSGMRIII NPGGKFVIGG PVGDAGLTGR KIIVDTYGGM ARHGGGAFSG
310 320 330 340 350
KDPSKVDRSA AYMLRWVAKN VVAAGLASRA EFQVAYAIGS ARPVGLYVDT
360 370 380 390 400
FGTATVPEEK IIEAVNAVFD LRPAAIVKEL NLLRPIYRRT AANGHFGRDD
410 420
ADFTWERTDR VEQLRAAAGL
Length:420
Mass (Da):44,759
Last modified:July 28, 2009 - v1
Checksum:iF722C4FA00BCDCB7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001628 Genomic DNA Translation: ACS30757.1

Genome annotation databases

EnsemblBacteriaiACS30757; ACS30757; Mlut_12520
KEGGimlu:Mlut_12520

Similar proteinsi

Entry informationi

Entry nameiC5CCE5_MICLC
AccessioniPrimary (citable) accession number: C5CCE5
Entry historyiIntegrated into UniProtKB/TrEMBL: July 28, 2009
Last sequence update: July 28, 2009
Last modified: May 23, 2018
This is version 69 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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