Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Dihydromethanopterin reductase

Gene

dmrA

Organism
Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of dihydromethanopterin (H2MPT) to tetrahydromethanopterin (H4MPT). Shows preference for NADPH rather than NADH as electron donor. Does not reduce dihydrofolate.2 Publications

Catalytic activityi

5,6,7,8-tetrahydromethanopterin + NAD(P)+ = 7,8-dihydromethanopterin + NAD(P)H.1 Publication

pH dependencei

Optimum pH is 5.3.1 Publication

Temperature dependencei

Optimum temperature is 30 degrees Celsius (at pH 5.3).1 Publication

Pathwayi: 5,6,7,8-tetrahydromethanopterin biosynthesis

This protein is involved in the pathway 5,6,7,8-tetrahydromethanopterin biosynthesis, which is part of Cofactor biosynthesis.1 Publication
View all proteins of this organism that are known to be involved in the pathway 5,6,7,8-tetrahydromethanopterin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei9NADP; via amide nitrogen and carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi16 – 21NADPBy similarity6
Nucleotide bindingi52 – 54NADPBy similarity3
Nucleotide bindingi93 – 97NADPBy similarity5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandNADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-18688
UniPathwayiUPA00065

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydromethanopterin reductase (EC:1.5.1.47)
Alternative name(s):
H(2)MPT reductase
Gene namesi
Name:dmrA
Ordered Locus Names:MexAM1_META1p4312
OrganismiMethylobacterium extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1)
Taxonomic identifieri272630 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
Proteomesi
  • UP000009081 Componenti: Chromosome

Pathology & Biotechi

Disruption phenotypei

Mutants have a C1-defective and methanol-sensitive phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004240831 – 135Dihydromethanopterin reductaseAdd BLAST135

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi272630.MexAM1_META1p4312

Structurei

3D structure databases

ProteinModelPortaliC5B2R8
SMRiC5B2R8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiENOG4105HJ3 Bacteria
ENOG4111QMF LUCA
HOGENOMiHOG000032899
KOiK17364
OMAiVRVICAI

Family and domain databases

Gene3Di3.40.430.10, 1 hit
InterProiView protein in InterPro
IPR024072 DHFR-like_dom_sf
IPR001796 DHFR_dom
PfamiView protein in Pfam
PF00186 DHFR_1, 1 hit
SUPFAMiSSF53597 SSF53597, 1 hit

Sequencei

Sequence statusi: Complete.

C5B2R8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIDVRCICAI GQRGQLGLNG HLPWEGNTDP LFVEDVTRFF ALTMGHVLIA
60 70 80 90 100
GPKTVASVPE FAFKDRTIDV IRSHEDPEAV LKRYPGRRIF VGGGIAVWNV
110 120 130
YAKYIQHWDV TRLPYDGEAD RWFDPAWLVG GPLRS
Length:135
Mass (Da):15,222
Last modified:July 28, 2009 - v1
Checksum:iA00C054D51CA5E5D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY093431 Genomic DNA Translation: AAM19724.1
CP001510 Genomic DNA Translation: ACS41948.1
RefSeqiWP_003606487.1, NC_012808.1

Genome annotation databases

EnsemblBacteriaiACS41948; ACS41948; MexAM1_META1p4312
KEGGimea:Mex_1p4312

Similar proteinsi

Entry informationi

Entry nameiDMRA_METEA
AccessioniPrimary (citable) accession number: C5B2R8
Secondary accession number(s): Q8KQF4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 16, 2013
Last sequence update: July 28, 2009
Last modified: February 28, 2018
This is version 39 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health