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Protein

Thiamine thiazole synthase

Gene

thi4

Organism
Thermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Cofactori

Fe2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei35NADUniRule annotation1
Binding sitei62NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei126NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi154Iron; shared with adjacent protomerUniRule annotation1
Metal bindingi169IronUniRule annotation1
Binding sitei216NAD; via amide nitrogenUniRule annotation1
Binding sitei226GlycineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi54 – 55NADUniRule annotation2
Nucleotide bindingi152 – 154NAD; shared with adjacent protomerUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotation
Biological processThiamine biosynthesisUniRule annotation
LigandIronUniRule annotation, Metal-bindingUniRule annotation, NADUniRule annotation

Enzyme and pathway databases

BioCyciTGAM593117:G1GUW-1303-MONOMER
UniPathwayiUPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthaseUniRule annotation (EC:4.-.-.-UniRule annotation)
Gene namesi
Name:thi4UniRule annotation
Ordered Locus Names:TGAM_1270Imported
OrganismiThermococcus gammatolerans (strain DSM 15229 / JCM 11827 / EJ3)Imported
Taxonomic identifieri593117 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaeThermococcus
Proteomesi
  • UP000001488 Componenti: Chromosome

PTM / Processingi

Proteomic databases

PaxDbiC5A6B0

Interactioni

Subunit structurei

Homooctamer; tetramer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi593117.TGAM_1270

Structurei

3D structure databases

ProteinModelPortaliC5A6B0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00574 Archaea
COG1635 LUCA
HOGENOMiHOG000106048
KOiK22699
OMAiMWGGGMM
OrthoDBiPOG093Z09PU

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_00304 Thi4, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR002922 Thi4_fam
IPR022828 Thi4_prok
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR00292 TIGR00292, 1 hit

Sequencei

Sequence statusi: Complete.

C5A6B0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLREIEISRA IIEAYTAELL ESLSLDVAIV GAGPSGMVAG YYLAKNGAKV
60 70 80 90 100
AIFEKKLSIG GGIWGGAMGF NKIVVQEEAR EILDEFGINY RPFGNGLYVA
110 120 130 140 150
DAIETATTIA SRAVKAGVRF FNMIEVEDLV LKNNRVAGIV INWTPVMRTG
160 170 180 190 200
LHVDPLTVEA RFVVDSTGHG AQVSQHLVRR GLLQVPGEGP MWAEKGEELT
210 220 230 240 250
VKHTREVFPG LYVTGMAANA IAGAPRMGPI FGGMFLSGRK AALEILEKLG
Length:250
Mass (Da):26,842
Last modified:July 28, 2009 - v1
Checksum:i2D6CA57ABA527C0A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001398 Genomic DNA Translation: ACS33772.1
RefSeqiWP_015858884.1, NC_012804.1

Genome annotation databases

EnsemblBacteriaiACS33772; ACS33772; TGAM_1270
GeneIDi7987916
KEGGitga:TGAM_1270
PATRICifig|593117.10.peg.1268

Similar proteinsi

Entry informationi

Entry nameiC5A6B0_THEGJ
AccessioniPrimary (citable) accession number: C5A6B0
Entry historyiIntegrated into UniProtKB/TrEMBL: July 28, 2009
Last sequence update: July 28, 2009
Last modified: July 18, 2018
This is version 60 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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