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Protein

Enolase

Gene

eno

Organism
Mycoplasma fermentans (strain ATCC 19989 / NBRC 14854 / NCTC 10117 / PG18)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.UniRule annotation

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.UniRule annotation

Cofactori

Mg2+UniRule annotation

Activity regulationi

The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI)
  4. Enolase (eno)
  5. Pyruvate kinase (MBIO_0233)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei165SubstrateUniRule annotation1
Binding sitei174SubstrateUniRule annotation1
Active sitei215Proton donorUniRule annotation1
Metal bindingi256MagnesiumUniRule annotation1
Metal bindingi318MagnesiumUniRule annotation1
Binding sitei318SubstrateUniRule annotation1
Metal bindingi345MagnesiumUniRule annotation1
Binding sitei345SubstrateUniRule annotation1
Active sitei370Proton acceptorUniRule annotation1
Binding sitei370Substrate (covalent); in inhibited formUniRule annotation1
Binding sitei421SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotation
Biological processGlycolysisUniRule annotation
LigandMagnesiumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

UniPathwayi
UPA00109;UER00187

Protein family/group databases

MoonProtiC4XEI3

Names & Taxonomyi

Protein namesi
Recommended name:
EnolaseUniRule annotation (EC:4.2.1.11UniRule annotation)
Alternative name(s):
2-phospho-D-glycerate hydro-lyaseUniRule annotation
2-phosphoglycerate dehydrataseUniRule annotation
Gene namesi
Name:enoUniRule annotation
Ordered Locus Names:MBIO_0290Imported
OrganismiMycoplasma fermentans (strain ATCC 19989 / NBRC 14854 / NCTC 10117 / PG18)Imported
Taxonomic identifieri496833 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000006810 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Secreted UniRule annotation
  • Cell surface UniRule annotation
  • Note: Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation, SecretedUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi496833.MBIO_0290

Structurei

3D structure databases

ProteinModelPortaliC4XEI3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 144Enolase_NInterPro annotationAdd BLAST135
Domaini149 – 458Enolase_CInterPro annotationAdd BLAST310

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni397 – 400Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the enolase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C70 Bacteria
COG0148 LUCA
KOiK01689
OMAiEFMIIPV

Family and domain databases

CDDicd03313 enolase, 1 hit
Gene3Di3.20.20.120, 1 hit
3.30.390.10, 1 hit
HAMAPiMF_00318 Enolase, 1 hit
InterProiView protein in InterPro
IPR000941 Enolase
IPR036849 Enolase-like_C_sf
IPR029017 Enolase-like_N
IPR034390 Enolase-like_superfamily
IPR020810 Enolase_C
IPR020809 Enolase_CS
IPR020811 Enolase_N
PANTHERiPTHR11902 PTHR11902, 1 hit
PfamiView protein in Pfam
PF00113 Enolase_C, 1 hit
PF03952 Enolase_N, 1 hit
PIRSFiPIRSF001400 Enolase, 1 hit
PRINTSiPR00148 ENOLASE
SFLDiSFLDG00178 enolase, 1 hit
SFLDS00001 Enolase, 1 hit
SMARTiView protein in SMART
SM01192 Enolase_C, 1 hit
SM01193 Enolase_N, 1 hit
SUPFAMiSSF51604 SSF51604, 1 hit
TIGRFAMsiTIGR01060 eno, 1 hit
PROSITEiView protein in PROSITE
PS00164 ENOLASE, 1 hit

Sequencei

Sequence statusi: Complete.

C4XEI3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKLRRKMSAI YKIHSREILD SRGNPTVQVE VWTEAGGYGL ANVPSGASTG
60 70 80 90 100
SKEALELRDQ GTKYEKNWFG GKGVMTAVDH VNKDIAPKLL GMEVTDQRAI
110 120 130 140 150
DMKMIKLDGT ATKSKFGANA ILGVSLAVAR AAADELQLPL YRYIGGTNAH
160 170 180 190 200
VLPLPMLNVM NGGAHASNTV DFQEFMIMPV GAKSIREALQ MANKVFHNLA
210 220 230 240 250
KLLKAAGHGT QVGDEGGFAP DCKSHEEVLD YLVQAIKAAG YKPATSGKDA
260 270 280 290 300
VAIAMDAASS EIYDEKSKKY VFKKLKKAIE AKHPGFEKLT NVKLEYTTDE
310 320 330 340 350
MIEYYGTLIA KYPIISIEDG LAEDDWAGFT KMTTKYGHQI QIVGDDLTVT
360 370 380 390 400
NPKLLARAIK EKAMNAILIK LNQIGSLTET IDAINMAQKA GMACVVSHRS
410 420 430 440 450
GETEDTTLAD VAVALNTGQI KTGSMSRTDR IAKYNRLLAI EEELDGADEF
460
EGREAFYNIK
Length:460
Mass (Da):50,089
Last modified:July 28, 2009 - v1
Checksum:i643A80A8D5904A17
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009608 Genomic DNA Translation: BAH69555.1

Genome annotation databases

EnsemblBacteriaiBAH69555; BAH69555; MBIO_0290
KEGGimfp:MBIO_0290
PATRICifig|496833.3.peg.716

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP009608 Genomic DNA Translation: BAH69555.1

3D structure databases

ProteinModelPortaliC4XEI3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi496833.MBIO_0290

Protein family/group databases

MoonProtiC4XEI3

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAH69555; BAH69555; MBIO_0290
KEGGimfp:MBIO_0290
PATRICifig|496833.3.peg.716

Phylogenomic databases

eggNOGiENOG4105C70 Bacteria
COG0148 LUCA
KOiK01689
OMAiEFMIIPV

Enzyme and pathway databases

UniPathwayi
UPA00109;UER00187

Family and domain databases

CDDicd03313 enolase, 1 hit
Gene3Di3.20.20.120, 1 hit
3.30.390.10, 1 hit
HAMAPiMF_00318 Enolase, 1 hit
InterProiView protein in InterPro
IPR000941 Enolase
IPR036849 Enolase-like_C_sf
IPR029017 Enolase-like_N
IPR034390 Enolase-like_superfamily
IPR020810 Enolase_C
IPR020809 Enolase_CS
IPR020811 Enolase_N
PANTHERiPTHR11902 PTHR11902, 1 hit
PfamiView protein in Pfam
PF00113 Enolase_C, 1 hit
PF03952 Enolase_N, 1 hit
PIRSFiPIRSF001400 Enolase, 1 hit
PRINTSiPR00148 ENOLASE
SFLDiSFLDG00178 enolase, 1 hit
SFLDS00001 Enolase, 1 hit
SMARTiView protein in SMART
SM01192 Enolase_C, 1 hit
SM01193 Enolase_N, 1 hit
SUPFAMiSSF51604 SSF51604, 1 hit
TIGRFAMsiTIGR01060 eno, 1 hit
PROSITEiView protein in PROSITE
PS00164 ENOLASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiC4XEI3_MYCFP
AccessioniPrimary (citable) accession number: C4XEI3
Entry historyiIntegrated into UniProtKB/TrEMBL: July 28, 2009
Last sequence update: July 28, 2009
Last modified: September 12, 2018
This is version 53 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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