UniProtKB - C4LK22 (SSRP_CORK4)
Protein
SsrA-binding protein
Gene
smpB
Organism
Corynebacterium kroppenstedtii (strain DSM 44385 / JCM 11950 / CIP 105744 / CCUG 35717)
Status
Functioni
Required for rescue of stalled ribosomes mediated by trans-translation. Binds to transfer-messenger RNA (tmRNA), required for stable association of tmRNA with ribosomes. tmRNA and SmpB together mimic tRNA shape, replacing the anticodon stem-loop with SmpB. tmRNA is encoded by the ssrA gene; the 2 termini fold to resemble tRNA(Ala) and it encodes a 'tag peptide', a short internal open reading frame. During trans-translation Ala-aminoacylated tmRNA acts like a tRNA, entering the A-site of stalled ribosomes, displacing the stalled mRNA. The ribosome then switches to translate the ORF on the tmRNA; the nascent peptide is terminated with the 'tag peptide' encoded by the tmRNA and targeted for degradation. The ribosome is freed to recommence translation, which seems to be the essential function of trans-translation.UniRule annotation
GO - Molecular functioni
- RNA binding Source: UniProtKB-UniRule
GO - Biological processi
- trans-translation Source: UniProtKB-UniRule
Keywordsi
Molecular function | RNA-binding |
Names & Taxonomyi
Protein namesi | Recommended name: SsrA-binding proteinUniRule annotationAlternative name(s): Small protein BUniRule annotation |
Gene namesi | Name:smpBUniRule annotation Ordered Locus Names:ckrop_1439 |
Organismi | Corynebacterium kroppenstedtii (strain DSM 44385 / JCM 11950 / CIP 105744 / CCUG 35717) |
Taxonomic identifieri | 645127 [NCBI] |
Taxonomic lineagei | Bacteria › Actinobacteria › Corynebacteriales › Corynebacteriaceae › Corynebacterium › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Note: The tmRNA-SmpB complex associates with stalled 70S ribosomes.UniRule annotation
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_1000201929 | 1 – 183 | SsrA-binding proteinAdd BLAST | 183 |
Family & Domainsi
Sequence similaritiesi
Belongs to the SmpB family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0691, Bacteria |
HOGENOMi | CLU_108953_2_1_11 |
OMAi | WTNHSAR |
OrthoDBi | 1720952at2 |
Family and domain databases
CDDi | cd09294, SmpB, 1 hit |
Gene3Di | 2.40.280.10, 1 hit |
HAMAPi | MF_00023, SmpB, 1 hit |
InterProi | View protein in InterPro IPR023620, SmpB IPR000037, SsrA-bd_prot IPR020081, SsrA-bd_prot_CS |
PANTHERi | PTHR30308, PTHR30308, 1 hit |
Pfami | View protein in Pfam PF01668, SmpB, 1 hit |
SUPFAMi | SSF74982, SSF74982, 1 hit |
TIGRFAMsi | TIGR00086, smpB, 1 hit |
PROSITEi | View protein in PROSITE PS01317, SSRP, 1 hit |
i Sequence
Sequence statusi: Complete.
C4LK22-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAKKATLVDH GAAKGKKKAQ SKVSKKNANK RLVVATNRRA RHDYTILDTW
60 70 80 90 100
EAGIQLVGTE VKSLREGKAS LIDSFATIDD GEVWLRHLHI PEYSKGHWTN
110 120 130 140 150
HSPRRTRKLL LHRNEIDSIM GKVRDGNNTL IPLSLYFSDG KLKVELALGR
160 170 180
GKQDYDRRQD IKRRTEEREA TRALGRRVKG LTG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP001620 Genomic DNA Translation: ACR18177.1 |
RefSeqi | WP_012732064.1, NC_012704.1 |
Genome annotation databases
EnsemblBacteriai | ACR18177; ACR18177; ckrop_1439 |
KEGGi | ckp:ckrop_1439 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CP001620 Genomic DNA Translation: ACR18177.1 |
RefSeqi | WP_012732064.1, NC_012704.1 |
3D structure databases
SMRi | C4LK22 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 645127.ckrop_1439 |
Genome annotation databases
EnsemblBacteriai | ACR18177; ACR18177; ckrop_1439 |
KEGGi | ckp:ckrop_1439 |
Phylogenomic databases
eggNOGi | COG0691, Bacteria |
HOGENOMi | CLU_108953_2_1_11 |
OMAi | WTNHSAR |
OrthoDBi | 1720952at2 |
Family and domain databases
CDDi | cd09294, SmpB, 1 hit |
Gene3Di | 2.40.280.10, 1 hit |
HAMAPi | MF_00023, SmpB, 1 hit |
InterProi | View protein in InterPro IPR023620, SmpB IPR000037, SsrA-bd_prot IPR020081, SsrA-bd_prot_CS |
PANTHERi | PTHR30308, PTHR30308, 1 hit |
Pfami | View protein in Pfam PF01668, SmpB, 1 hit |
SUPFAMi | SSF74982, SSF74982, 1 hit |
TIGRFAMsi | TIGR00086, smpB, 1 hit |
PROSITEi | View protein in PROSITE PS01317, SSRP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SSRP_CORK4 | |
Accessioni | C4LK22Primary (citable) accession number: C4LK22 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 22, 2009 |
Last sequence update: | July 7, 2009 | |
Last modified: | December 2, 2020 | |
This is version 66 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families