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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Corynebacterium kroppenstedtii (strain DSM 44385 / JCM 11950 / CIP 105744 / CCUG 35717)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei82SubstrateUniRule annotation1
Metal bindingi229ManganeseUniRule annotation1
Metal bindingi249Manganese; via tele nitrogenUniRule annotation1
Binding sitei271SubstrateUniRule annotation1
Active sitei273UniRule annotation1
Metal bindingi296ManganeseUniRule annotation1
Binding sitei388GTPUniRule annotation1
Binding sitei419GTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi272 – 277GTPUniRule annotation6
Nucleotide bindingi514 – 517GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylase, Lyase
Biological processGluconeogenesis
LigandGTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCKRO645127:CKROP_RS01235-MONOMER
UniPathwayi
UPA00138

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:ckrop_0253
OrganismiCorynebacterium kroppenstedtii (strain DSM 44385 / JCM 11950 / CIP 105744 / CCUG 35717)
Taxonomic identifieri645127 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000001473 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002062321 – 621Phosphoenolpyruvate carboxykinase [GTP]Add BLAST621

Proteomic databases

PRIDEiC4LGT5

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi645127.ckrop_0253

Structurei

3D structure databases

SMRiC4LGT5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni220 – 222Substrate bindingUniRule annotation3
Regioni386 – 388Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0M Bacteria
COG1274 LUCA
HOGENOMiHOG000191700
KOiK01596
OMAiCWLALTD
OrthoDBiPOG091H0B4F

Family and domain databases

CDDicd00819 PEPCK_GTP, 1 hit
Gene3Di3.40.449.10, 2 hits
3.90.228.20, 2 hits
HAMAPiMF_00452 PEPCK_GTP, 1 hit
InterProiView protein in InterPro
IPR013035 PEP_carboxykinase_C
IPR008209 PEP_carboxykinase_GTP
IPR035077 PEP_carboxykinase_GTP_C
IPR035078 PEP_carboxykinase_GTP_N
IPR008210 PEP_carboxykinase_N
PANTHERiPTHR11561 PTHR11561, 1 hit
PfamiView protein in Pfam
PF00821 PEPCK_C, 1 hit
PF17297 PEPCK_N, 1 hit
PIRSFiPIRSF001348 PEP_carboxykinase_GTP, 1 hit
SUPFAMiSSF68923 SSF68923, 1 hit

Sequencei

Sequence statusi: Complete.

C4LGT5-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSAPTIPGLE GNAPTTNQDM LNWIAECAEL CQPDKVVFCD GSDEEWEAIA
60 70 80 90 100
KDLVEKGTLI KLNEEKRPNS YLASSDPADV ARVESRTFIC SKTEDGAGPT
110 120 130 140 150
NNWRDPDEMR AEMSEHFKGS MKGRTMYVVP FCMGPITDPD PKLGIELTDS
160 170 180 190 200
GYVVMSMRIM TRMGKEALDK IGDGPFVKGL HSVGAPLEPG QEDVKWPCNE
210 220 230 240 250
TKYITQFPED RVIWSYGSGY GGNAILAKKC YALRIASVMA KDEGWMAEHM
260 270 280 290 300
LILKLISPEG KAYHICAAFP SQCGKTNLAM IQPTIPGWKA EVIGDDIAWL
310 320 330 340 350
HFGDDGRLYA VNPENGFFGV APGTNYKSNP NAMKTMEAGN NIFTNVALTD
360 370 380 390 400
DGDVWWEGLE NEPDHLIDWK GKDWTPDEDC LSSHPNSRYC VPIEQCPVAA
410 420 430 440 450
PEFNDPKGVP VDAILFGGRR PDTVPLVTEA IDWQHATFIG ATLASGQTAA
460 470 480 490 500
AAEAKVGTLR YDPMAMLPFI GYNVGDYLQH WLDIGKKGGD KLPKVFLVNW
510 520 530 540 550
FRRGDDGRFL WPGFGENSRV LKWIVDRIEG RVDAKKTVVG YTAYSKDIDI
560 570 580 590 600
DGLDTDPKDV KAALTAPADK WQMDLSDTED WLKSLGPKVP QEIWDEFDFL
610 620
KTRIKTANKD GNKALDNMKT K
Length:621
Mass (Da):68,814
Last modified:July 7, 2009 - v1
Checksum:iD70178D80F67B5FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001620 Genomic DNA Translation: ACR17040.1
RefSeqiWP_012730928.1, NC_012704.1

Genome annotation databases

EnsemblBacteriaiACR17040; ACR17040; ckrop_0253
KEGGickp:ckrop_0253

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001620 Genomic DNA Translation: ACR17040.1
RefSeqiWP_012730928.1, NC_012704.1

3D structure databases

SMRiC4LGT5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi645127.ckrop_0253

Proteomic databases

PRIDEiC4LGT5

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACR17040; ACR17040; ckrop_0253
KEGGickp:ckrop_0253

Phylogenomic databases

eggNOGiENOG4105C0M Bacteria
COG1274 LUCA
HOGENOMiHOG000191700
KOiK01596
OMAiCWLALTD
OrthoDBiPOG091H0B4F

Enzyme and pathway databases

UniPathwayi
UPA00138

BioCyciCKRO645127:CKROP_RS01235-MONOMER

Family and domain databases

CDDicd00819 PEPCK_GTP, 1 hit
Gene3Di3.40.449.10, 2 hits
3.90.228.20, 2 hits
HAMAPiMF_00452 PEPCK_GTP, 1 hit
InterProiView protein in InterPro
IPR013035 PEP_carboxykinase_C
IPR008209 PEP_carboxykinase_GTP
IPR035077 PEP_carboxykinase_GTP_C
IPR035078 PEP_carboxykinase_GTP_N
IPR008210 PEP_carboxykinase_N
PANTHERiPTHR11561 PTHR11561, 1 hit
PfamiView protein in Pfam
PF00821 PEPCK_C, 1 hit
PF17297 PEPCK_N, 1 hit
PIRSFiPIRSF001348 PEP_carboxykinase_GTP, 1 hit
SUPFAMiSSF68923 SSF68923, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPCKG_CORK4
AccessioniPrimary (citable) accession number: C4LGT5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 7, 2009
Last modified: October 10, 2018
This is version 57 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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