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Protein

RNA-directed RNA polymerase catalytic subunit

Gene

PB1

Organism
Influenza A virus (A/California/07/2009(H1N1))
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

RNA-dependent RNA polymerase which is responsible for replication and transcription of virus RNA segments. The transcription of viral mRNAs occurs by a unique mechanism called cap-snatching. 5' methylated caps of cellular mRNAs are cleaved after 10-13 nucleotides by PA. In turn, these short capped RNAs are used as primers by PB1 for transcription of viral mRNAs. During virus replication, PB1 initiates RNA synthesis and copies vRNA into complementary RNA (cRNA) which in turn serves as a template for the production of more vRNAs.UniRule annotation

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).UniRule annotationSAAS annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, RNA-directed RNA polymeraseUniRule annotationSAAS annotation, Transferase
Biological processEukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of host RNA polymerase II by virusUniRule annotation, Viral RNA replicationUniRule annotationSAAS annotation, Viral transcriptionUniRule annotation
LigandNucleotide-bindingUniRule annotationSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase catalytic subunitUniRule annotation (EC:2.7.7.48UniRule annotation)
Alternative name(s):
Polymerase basic protein 1UniRule annotation
Short name:
PB1UniRule annotation
RNA-directed RNA polymerase subunit P1UniRule annotation
Gene namesi
Name:PB1UniRule annotationImported
OrganismiInfluenza A virus (A/California/07/2009(H1N1))Imported
Taxonomic identifieri641809 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesOrthomyxoviridaeAlphainfluenzavirus
Proteomesi
  • UP000206460 Componenti: Genome
  • UP000170266 Componenti: Genome
  • UP000207659 Componenti: Genome
  • UP000206440 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasmUniRule annotation, Host nucleusUniRule annotation

PTM / Processingi

Post-translational modificationi

Phosphorylated by host PRKCA.UniRule annotation

Keywords - PTMi

PhosphoproteinUniRule annotation

Interactioni

Subunit structurei

Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA. Interacts (via N-terminus) with PA (via C-terminus). Interacts (via C-terminus) with PB2 (via N-terminus); this interaction is essential for transcription initiation.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini286 – 483RdRp catalyticInterPro annotationAdd BLAST198

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni249 – 256Promoter-binding siteUniRule annotation8

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi187 – 195Nuclear localization signalUniRule annotation9
Motifi203 – 216Nuclear localization signalUniRule annotationAdd BLAST14

Sequence similaritiesi

Belongs to the influenza viruses polymerase PB1 family.UniRule annotationSAAS annotation

Family and domain databases

HAMAPiMF_04065 INFV_RDRP, 1 hit
InterProiView protein in InterPro
IPR007099 RNA-dir_pol_NSvirus
IPR001407 RNA_pol_PB1_influenza
PfamiView protein in Pfam
PF00602 Flu_PB1, 1 hit
PIRSFiPIRSF000827 RdRPol_OMV, 1 hit
PROSITEiView protein in PROSITE
PS50525 RDRP_SSRNA_NEG_SEG, 1 hit

Sequencei

Sequence statusi: Complete.

C3W5X7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDVNPTLLFL KIPAQNAIST TFPYTGDPPY SHGTGTGYTM DTVNRTHQYS
60 70 80 90 100
EKGKWTTNTE TGAPQLNPID GPLPEDNEPS GYAQTDCVLE AMAFLEESHP
110 120 130 140 150
GIFENSCLET MEVVQQTRVD KLTQGRQTYD WTLNRNQPAA TALANTIEVF
160 170 180 190 200
RSNGLTANES GRLIDFLKDV MESMNKEEIE ITTHFQRKRR VRDNMTKKMV
210 220 230 240 250
TQRTIGKKKQ RLNKRGYLIR ALTLNTMTKD AERGKLKRRA IATPGMQIRG
260 270 280 290 300
FVYFVETLAR SICEKLEQSG LPVGGNEKKA KLANVVRKMM TNSQDTEISF
310 320 330 340 350
TITGDNTKWN ENQNPRMFLA MITYITRNQP EWFRNILSMA PIMFSNKMAR
360 370 380 390 400
LGKGYMFESK RMKIRTQIPA EMLASIDLKY FNESTKKKIE KIRPLLIDGT
410 420 430 440 450
ASLSPGMMMG MFNMLSTVLG VSILNLGQKK YTKTIYWWDG LQSSDDFALI
460 470 480 490 500
VNAPNHEGIQ AGVDRFYRTC KLVGINMSKK KSYINKTGTF EFTSFFYRYG
510 520 530 540 550
FVANFSMELP SFGVSGVNES ADMSIGVTVI KNNMINNDLG PATAQMALQL
560 570 580 590 600
FIKDYRYTYR CHRGDTQIQT RRSFELKKLW DQTQSKVGLL VSDGGPNLYN
610 620 630 640 650
IRNLHIPEVC LKWELMDDDY RGRLCNPLNP FVSHKEIDSV NNAVVMPAHG
660 670 680 690 700
PAKSMEYDAV ATTHSWIPKR NRSILNTSQR GILEDEQMYQ KCCNLFEKFF
710 720 730 740 750
PSSSYRRPVG ISSMVEAMVS RARIDARVDF ESGRIKKEEF SEIMKICSTI

EELRRQK
Length:757
Mass (Da):86,394
Last modified:June 16, 2009 - v1
Checksum:iE99C4D5A76832463
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FJ966978 Viral cRNA Translation: ACP41958.1
FJ969531 Viral cRNA Translation: ACP44176.1
GQ323558 Viral cRNA Translation: ACS72613.1
CY121686 Viral cRNA Translation: AFM72840.1
KC781787 Viral cRNA Translation: AGI54795.1
KU933483 Viral cRNA Translation: AMV49020.1
CY266197 Viral cRNA Translation: AUR34090.1
RefSeqiYP_009118628.1, NC_026435.1

Genome annotation databases

GeneIDi23308122
KEGGivg:23308122

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FJ966978 Viral cRNA Translation: ACP41958.1
FJ969531 Viral cRNA Translation: ACP44176.1
GQ323558 Viral cRNA Translation: ACS72613.1
CY121686 Viral cRNA Translation: AFM72840.1
KC781787 Viral cRNA Translation: AGI54795.1
KU933483 Viral cRNA Translation: AMV49020.1
CY266197 Viral cRNA Translation: AUR34090.1
RefSeqiYP_009118628.1, NC_026435.1

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi23308122
KEGGivg:23308122

Family and domain databases

HAMAPiMF_04065 INFV_RDRP, 1 hit
InterProiView protein in InterPro
IPR007099 RNA-dir_pol_NSvirus
IPR001407 RNA_pol_PB1_influenza
PfamiView protein in Pfam
PF00602 Flu_PB1, 1 hit
PIRSFiPIRSF000827 RdRPol_OMV, 1 hit
PROSITEiView protein in PROSITE
PS50525 RDRP_SSRNA_NEG_SEG, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiC3W5X7_9INFA
AccessioniPrimary (citable) accession number: C3W5X7
Entry historyiIntegrated into UniProtKB/TrEMBL: June 16, 2009
Last sequence update: June 16, 2009
Last modified: April 25, 2018
This is version 51 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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