UniProtKB - C3SU43 (C3SU43_ECOLX)
Protein
Multifunctional CCA protein
Gene
cca
Organism
Escherichia coli
Status
Functioni
Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate. Also shows phosphatase, 2'-nucleotidase and 2',3'-cyclic phosphodiesterase activities. These phosphohydrolase activities are probably involved in the repair of the tRNA 3'-CCA terminus degraded by intracellular RNases.UniRule annotationSAAS annotation
Miscellaneous
A single active site specifically recognizes both ATP and CTP and is responsible for their addition.UniRule annotation
Catalytic activityi
- a tRNA precursor + ATP + 2 CTP = a tRNA with a 3' CCA end + 3 diphosphateUniRule annotationSAAS annotationEC:2.7.7.72UniRule annotationSAAS annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 8 | ATP or CTP; via amide nitrogenUniRule annotation | 1 | |
Binding sitei | 11 | ATP or CTPUniRule annotation | 1 | |
Metal bindingi | 21 | MagnesiumUniRule annotation | 1 | |
Metal bindingi | 23 | MagnesiumUniRule annotation | 1 | |
Binding sitei | 91 | ATP or CTPUniRule annotation | 1 | |
Binding sitei | 137 | ATP or CTPUniRule annotation | 1 | |
Binding sitei | 140 | ATP or CTPUniRule annotation | 1 |
GO - Molecular functioni
- ATP:3'-cytidine-cytidine-tRNA adenylyltransferase activity Source: UniProtKB-EC
- ATP binding Source: UniProtKB-UniRule
- CTP:3'-cytidine-tRNA cytidylyltransferase activity Source: UniProtKB-EC
- CTP:tRNA cytidylyltransferase activity Source: UniProtKB-EC
- cyclic-nucleotide phosphodiesterase activity Source: UniProtKB-UniRule
- magnesium ion binding Source: UniProtKB-UniRule
- phosphatase activity Source: UniProtKB-UniRule
- tRNA binding Source: UniProtKB-UniRule
- tRNA cytidylyltransferase activity Source: UniProtKB-UniRule
GO - Biological processi
- RNA repair Source: UniProtKB-KW
- tRNA 3'-terminal CCA addition Source: UniProtKB-UniRule
Keywordsi
Molecular function | HydrolaseUniRule annotationSAAS annotationImported, Multifunctional enzymeUniRule annotationSAAS annotation, NucleotidyltransferaseUniRule annotationSAAS annotationImported, RNA-bindingUniRule annotationSAAS annotation, Transferase |
Biological process | RNA repairUniRule annotationSAAS annotation, tRNA processingUniRule annotationSAAS annotation |
Ligand | ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, NickelUniRule annotationSAAS annotation, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | GCF_001865295:AU473_RS06415-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Multifunctional CCA proteinUniRule annotationIncluding the following 4 domains: Alternative name(s): CCA tRNA nucleotidyltransferaseUniRule annotation tRNA CCA-pyrophosphorylaseUniRule annotation tRNA adenylyl-/cytidylyl-transferaseUniRule annotation tRNA nucleotidyltransferaseUniRule annotation tRNA-NTUniRule annotation |
Gene namesi | Name:ccaUniRule annotationImported ORF Names:ARC77_11720Imported, AU473_06430Imported, BTQ04_03885Imported, BvCms35BK_02950Imported, BWP17_06615Imported, C9E67_04175Imported, CRE06_06105Imported, CVH05_25465Imported, D4M06_13735Imported, D7K63_18215Imported, D7K66_03820Imported, D9L89_04695Imported, D9X97_02445Imported, DAH26_15925Imported, DAH49_14610Imported, DL455_14695Imported, DMI04_03545Imported, DNB37_07935Imported, DOY56_09375Imported, DXX80_008905Imported, EB509_06025Imported, EB510_08115Imported, EB515_06350Imported, ECs3939Imported, ED225_03595Imported, ED607_04590Imported, ED611_03605Imported, ED903_02205Imported, ED944_06585Imported, EEA45_08250Imported, EF173_13505Imported, NCTC13127_01043Imported |
Organismi | Escherichia coliImported |
Taxonomic identifieri | 562 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia |
Proteomesi |
|
Interactioni
Subunit structurei
Monomer. Can also form homodimers and oligomers.
UniRule annotationSAAS annotationFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 228 – 329 | HDInterPro annotationAdd BLAST | 102 |
Domaini
Comprises two domains: an N-terminal domain containing the nucleotidyltransferase activity and a C-terminal HD domain associated with both phosphodiesterase and phosphatase activities.UniRule annotation
Sequence similaritiesi
Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily.UniRule annotationSAAS annotation
Phylogenomic databases
eggNOGi | ENOG4105D4J Bacteria COG0617 LUCA |
Family and domain databases
CDDi | cd00077 HDc, 1 hit cd05398 NT_ClassII-CCAase, 1 hit |
HAMAPi | MF_01261 CCA_bact_type1, 1 hit MF_01262 CCA_bact_type2, 1 hit |
InterProi | View protein in InterPro IPR012006 CCA_bact IPR003607 HD/PDEase_dom IPR006674 HD_domain IPR002646 PolA_pol_head_dom IPR032828 PolyA_RNA-bd |
Pfami | View protein in Pfam PF01966 HD, 1 hit PF01743 PolyA_pol, 1 hit PF12627 PolyA_pol_RNAbd, 1 hit |
PIRSFi | PIRSF000813 CCA_bact, 1 hit |
PROSITEi | View protein in PROSITE PS51831 HD, 1 hit |
i Sequence
Sequence statusi: Complete.
C3SU43-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKIYLVGGAV RDALLGLPVK DRDWVVVGST PQEMLDAGYQ QVGRDFPVFL
60 70 80 90 100
HPQTHEEYAL ARTERKSGSG YTGFTCYAAP DVTLEDDLKR RDLTINALAQ
110 120 130 140 150
DDNGEIIDPY NGLGDLQNRL LRHVSPAFGE DPLRVLRVAR FAARYAHLGF
160 170 180 190 200
RIADETLTLM REMTHAGELE HLTPERVWKE TENALTTRNP QVFFQVLRDC
210 220 230 240 250
GALRVLFPEI DALFGVPAPA RWHPEIDTGI HTLMTLSMAA MLSPQVDVRF
260 270 280 290 300
TTLCHDLGKG LTPPELWPRH HGHGPAGVKL VEQLCQRLRV PNEIRDLARL
310 320 330 340 350
VAEFHDLIHT FPMLNPKTIV KLFDSIDAWR KPQRVEQLAL TSEADVRGRT
360 370 380 390 400
GFESADYPQG RWLREAWEVA QSVPTKAVVE AGFKGVEIRE ELTRRRIAAV
410
ASWKEQRCPK PE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EU895421 Genomic DNA Translation: ACI77982.1 EU895422 Genomic DNA Translation: ACI77983.1 EU895424 Genomic DNA Translation: ACI77985.1 CP015020 Genomic DNA Translation: AMW42856.1 CP017669 Genomic DNA Translation: APA40557.1 BIAN01000085 Genomic DNA Translation: GCH48299.1 RLEX01000008 Genomic DNA Translation: MDQ89066.1 RLEV01000004 Genomic DNA Translation: MDS17233.1 RLJY01000009 Genomic DNA Translation: MDW74126.1 RMHX01000003 Genomic DNA Translation: MEV95167.1 RMNN01000010 Genomic DNA Translation: MFC81752.1 RMOH01000004 Genomic DNA Translation: MFD98408.1 RNHG01000003 Genomic DNA Translation: MFY58149.1 RNHF01000007 Genomic DNA Translation: MFZ53479.1 RNJD01000004 Genomic DNA Translation: MGB96511.1 RTBW01000018 Genomic DNA Translation: MJD40069.1 RTDX01000003 Genomic DNA Translation: MJF34846.1 RTHS01000008 Genomic DNA Translation: MJK80882.1 RTMR01000009 Genomic DNA Translation: MJR33733.1 RTVT01000017 Genomic DNA Translation: MKB18972.1 RUOF01000002 Genomic DNA Translation: MKX24598.1 RVQE01000003 Genomic DNA Translation: MME82373.1 RVVI01000018 Genomic DNA Translation: MML49724.1 RVZZ01000003 Genomic DNA Translation: MMR44333.1 RWFH01000009 Genomic DNA Translation: MMW37782.1 MTFS01000006 Genomic DNA Translation: OOC82912.1 MRVX01000011 Genomic DNA Translation: PAU17720.1 PGUD01000305 Genomic DNA Translation: PJG30902.1 CP028607 Genomic DNA Translation: QCL46458.1 PDAQ01000004 Genomic DNA Translation: RFQ88394.1 UGCM01000003 Genomic DNA Translation: STI63365.1 QAFU01000010 Genomic DNA Translation: TGF12889.1 QAEX01000011 Genomic DNA Translation: TGG25519.1 QSMR02000002 Genomic DNA Translation: TKT35625.1 |
RefSeqi | WP_000708496.1, NZ_VODI01000123.1 |
Genome annotation databases
EnsemblBacteriai | AMW42856; AMW42856; ARC77_11720 OEG68525; OEG68525; A7H95_02125 |
PATRICi | fig|562.7073.peg.458 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EU895421 Genomic DNA Translation: ACI77982.1 EU895422 Genomic DNA Translation: ACI77983.1 EU895424 Genomic DNA Translation: ACI77985.1 CP015020 Genomic DNA Translation: AMW42856.1 CP017669 Genomic DNA Translation: APA40557.1 BIAN01000085 Genomic DNA Translation: GCH48299.1 RLEX01000008 Genomic DNA Translation: MDQ89066.1 RLEV01000004 Genomic DNA Translation: MDS17233.1 RLJY01000009 Genomic DNA Translation: MDW74126.1 RMHX01000003 Genomic DNA Translation: MEV95167.1 RMNN01000010 Genomic DNA Translation: MFC81752.1 RMOH01000004 Genomic DNA Translation: MFD98408.1 RNHG01000003 Genomic DNA Translation: MFY58149.1 RNHF01000007 Genomic DNA Translation: MFZ53479.1 RNJD01000004 Genomic DNA Translation: MGB96511.1 RTBW01000018 Genomic DNA Translation: MJD40069.1 RTDX01000003 Genomic DNA Translation: MJF34846.1 RTHS01000008 Genomic DNA Translation: MJK80882.1 RTMR01000009 Genomic DNA Translation: MJR33733.1 RTVT01000017 Genomic DNA Translation: MKB18972.1 RUOF01000002 Genomic DNA Translation: MKX24598.1 RVQE01000003 Genomic DNA Translation: MME82373.1 RVVI01000018 Genomic DNA Translation: MML49724.1 RVZZ01000003 Genomic DNA Translation: MMR44333.1 RWFH01000009 Genomic DNA Translation: MMW37782.1 MTFS01000006 Genomic DNA Translation: OOC82912.1 MRVX01000011 Genomic DNA Translation: PAU17720.1 PGUD01000305 Genomic DNA Translation: PJG30902.1 CP028607 Genomic DNA Translation: QCL46458.1 PDAQ01000004 Genomic DNA Translation: RFQ88394.1 UGCM01000003 Genomic DNA Translation: STI63365.1 QAFU01000010 Genomic DNA Translation: TGF12889.1 QAEX01000011 Genomic DNA Translation: TGG25519.1 QSMR02000002 Genomic DNA Translation: TKT35625.1 |
RefSeqi | WP_000708496.1, NZ_VODI01000123.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Genome annotation databases
EnsemblBacteriai | AMW42856; AMW42856; ARC77_11720 OEG68525; OEG68525; A7H95_02125 |
PATRICi | fig|562.7073.peg.458 |
Phylogenomic databases
eggNOGi | ENOG4105D4J Bacteria COG0617 LUCA |
Enzyme and pathway databases
BioCyci | GCF_001865295:AU473_RS06415-MONOMER |
Family and domain databases
CDDi | cd00077 HDc, 1 hit cd05398 NT_ClassII-CCAase, 1 hit |
HAMAPi | MF_01261 CCA_bact_type1, 1 hit MF_01262 CCA_bact_type2, 1 hit |
InterProi | View protein in InterPro IPR012006 CCA_bact IPR003607 HD/PDEase_dom IPR006674 HD_domain IPR002646 PolA_pol_head_dom IPR032828 PolyA_RNA-bd |
Pfami | View protein in Pfam PF01966 HD, 1 hit PF01743 PolyA_pol, 1 hit PF12627 PolyA_pol_RNAbd, 1 hit |
PIRSFi | PIRSF000813 CCA_bact, 1 hit |
PROSITEi | View protein in PROSITE PS51831 HD, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | C3SU43_ECOLX | |
Accessioni | C3SU43Primary (citable) accession number: C3SU43 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | June 16, 2009 |
Last sequence update: | June 16, 2009 | |
Last modified: | November 13, 2019 | |
This is version 88 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |