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Protein

Methylated-DNA--protein-cysteine methyltransferase

Gene

ogt

Organism
Sulfolobus islandicus (strain Y.N.15.51 / Yellowstone #2)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.UniRule annotation

Miscellaneous

This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.

Catalytic activityi

DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.UniRule annotation
DNA (containing 4-O-methylthymine) + protein L-cysteine = DNA (without 4-O-methylthymine) + protein S-methyl-L-cysteine.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei119Nucleophile; methyl group acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processDNA damage, DNA repair

Enzyme and pathway databases

BioCyciSISL419942:G1GV0-2829-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Methylated-DNA--protein-cysteine methyltransferaseUniRule annotation (EC:2.1.1.63UniRule annotation)
Alternative name(s):
6-O-methylguanine-DNA methyltransferaseUniRule annotation
Short name:
MGMTUniRule annotation
O-6-methylguanine-DNA-alkyltransferaseUniRule annotation
Gene namesi
Name:ogtUniRule annotation
Ordered Locus Names:YN1551_2888
OrganismiSulfolobus islandicus (strain Y.N.15.51 / Yellowstone #2)
Taxonomic identifieri419942 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000006818 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002129051 – 151Methylated-DNA--protein-cysteine methyltransferaseAdd BLAST151

Structurei

3D structure databases

ProteinModelPortaliC3NMY2
SMRiC3NMY2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MGMT family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000244137
KOiK00567
OMAiYGESRSY
OrthoDBiPOG093Z0E1E

Family and domain databases

CDDicd06445 ATase, 1 hit
Gene3Di1.10.10.10, 1 hit
HAMAPiMF_00772 OGT, 1 hit
InterProiView protein in InterPro
IPR001497 MethylDNA_cys_MeTrfase_AS
IPR014048 MethylDNA_cys_MeTrfase_DNA-bd
IPR036217 MethylDNA_cys_MeTrfase_DNAb
IPR023546 MGMT
IPR036631 MGMT_N_sf
IPR036388 WH-like_DNA-bd_sf
PfamiView protein in Pfam
PF01035 DNA_binding_1, 1 hit
SUPFAMiSSF46767 SSF46767, 1 hit
SSF53155 SSF53155, 1 hit
TIGRFAMsiTIGR00589 ogt, 1 hit
PROSITEiView protein in PROSITE
PS00374 MGMT, 1 hit

Sequencei

Sequence statusi: Complete.

C3NMY2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLVYGLYKSP LGYITVAKDD KGFIMLDFCD CVEGNSRDDS SFTEFFHKLD
60 70 80 90 100
LYFEGKPINL REPINLKTYP FRLSVFKEVM KIPWGKVMPY KQIADSLGTS
110 120 130 140 150
PRAVGMALSK NPILLIIPCH RVIAENGIGG YSRGVKLKRA LLELEGVKIP

E
Length:151
Mass (Da):17,033
Last modified:June 16, 2009 - v1
Checksum:i8451408CD5BC8B9D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001404 Genomic DNA Translation: ACP49789.1
RefSeqiWP_012718143.1, NC_012623.1

Genome annotation databases

EnsemblBacteriaiACP49789; ACP49789; YN1551_2888
GeneIDi7808881
KEGGisin:YN1551_2888

Similar proteinsi

Entry informationi

Entry nameiOGT_SULIN
AccessioniPrimary (citable) accession number: C3NMY2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: June 16, 2009
Last modified: February 28, 2018
This is version 54 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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