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Protein

Thiamine thiazole synthase

Gene

thi4

Organism
Sulfolobus islandicus (strain M.14.25 / Kamchatka #1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.UniRule annotation

Cofactori

Fe2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei41NADUniRule annotation1
Binding sitei68NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei132NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi162Iron; shared with adjacent protomerUniRule annotation1
Metal bindingi177IronUniRule annotation1
Binding sitei227NAD; via amide nitrogenUniRule annotation1
Binding sitei237GlycineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi60 – 61NADUniRule annotation2
Nucleotide bindingi160 – 162NAD; shared with adjacent protomerUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processThiamine biosynthesis
LigandIron, Metal-binding, NAD

Enzyme and pathway databases

BioCyciSISL427317:G1GUY-1721-MONOMER
UniPathwayi
UPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthaseUniRule annotation (EC:4.-.-.-UniRule annotation)
Gene namesi
Name:thi4UniRule annotation
Ordered Locus Names:M1425_1670
OrganismiSulfolobus islandicus (strain M.14.25 / Kamchatka #1)
Taxonomic identifieri427317 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001350 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002050081 – 267Thiamine thiazole synthaseAdd BLAST267

Interactioni

Subunit structurei

Homooctamer; tetramer of dimers.UniRule annotation

Structurei

3D structure databases

SMRiC3MWW9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000106048
KOiK22699
OMAiMTEFTDI
OrthoDBiPOG093Z09PU

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_00304 Thi4, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR002922 Thi4_fam
IPR022828 Thi4_prok
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR00292 TIGR00292, 1 hit

Sequencei

Sequence statusi: Complete.

C3MWW9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEVKIKQVEE VKISRYIIKE TMEDWYQFVE SDVVIVGAGP SGLSAAYYLA
60 70 80 90 100
KAGLKTLVFE RRLSFGGGIG GGAMLFHKLI IEKPADEILR EVNVRLKEVE
110 120 130 140 150
EGVYVVDSAE FMAKLATAAI DAGAKIIHGV TVDDVIFREN PLRVAGVAVE
160 170 180 190 200
WTATQMASLH VDPIFISAKA VVDATGHDAE VISVAARKIP ELGIVIPGEK
210 220 230 240 250
SAYSERAEEL TVINTGKVAE GLYAAGMAVT EVKGLPRMGP IFGAMVLSGK
260
AVAEEITKDL LKSEIRT
Length:267
Mass (Da):28,702
Last modified:June 16, 2009 - v1
Checksum:i4EB28B391187655A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001400 Genomic DNA Translation: ACP38419.1
RefSeqiWP_012711650.1, NC_012588.1

Genome annotation databases

EnsemblBacteriaiACP38419; ACP38419; M1425_1670
GeneIDi7794128
KEGGisia:M1425_1670

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001400 Genomic DNA Translation: ACP38419.1
RefSeqiWP_012711650.1, NC_012588.1

3D structure databases

SMRiC3MWW9
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACP38419; ACP38419; M1425_1670
GeneIDi7794128
KEGGisia:M1425_1670

Phylogenomic databases

HOGENOMiHOG000106048
KOiK22699
OMAiMTEFTDI
OrthoDBiPOG093Z09PU

Enzyme and pathway databases

UniPathwayi
UPA00060

BioCyciSISL427317:G1GUY-1721-MONOMER

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_00304 Thi4, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR002922 Thi4_fam
IPR022828 Thi4_prok
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR00292 TIGR00292, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTHI4_SULIM
AccessioniPrimary (citable) accession number: C3MWW9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: June 16, 2009
Last modified: October 10, 2018
This is version 57 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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