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UniProtKB - C1KEU0 (METE_KALTU)
Protein
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
Gene
N/A
Organism
Kali turgidum (Prickly saltwort) (Salsola kali)
Status
Functioni
Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.
By similarityCatalytic activityi
- 5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = L-methionine + tetrahydropteroyltri-L-glutamateBy similarityEC:2.1.1.14By similarity
Cofactori
Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity
: L-methionine biosynthesis via de novo pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes L-methionine from L-homocysteine (MetE route).By similarity This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from L-homocysteine (MetE route), the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 18 | 5-methyltetrahydropteroyltri-L-glutamateBy similarity | 1 | |
Binding sitei | 116 | 5-methyltetrahydropteroyltri-L-glutamateBy similarity | 1 | |
Binding sitei | 490 | L-methionineBy similarity | 1 | |
Binding sitei | 567 | 5-methyltetrahydropteroyltri-L-glutamateBy similarity | 1 | |
Binding sitei | 605 | L-homocysteineBy similarity | 1 | |
Binding sitei | 605 | L-methionineBy similarity | 1 | |
Metal bindingi | 647 | Zinc 1; catalytic; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 649 | Zinc 1; catalyticBy similarity | 1 | |
Metal bindingi | 658 | Zinc 2; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 662 | Zinc 2By similarity | 1 | |
Metal bindingi | 671 | Zinc 1; catalyticBy similarity | 1 | |
Active sitei | 701 | Proton donorBy similarity | 1 | |
Metal bindingi | 733 | Zinc 1; catalyticBy similarity | 1 |
GO - Molecular functioni
- 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Source: UniProtKB-EC
- zinc ion binding Source: InterPro
GO - Biological processi
- methionine biosynthetic process Source: UniProtKB-KW
- methylation Source: UniProtKB-KW
Keywordsi
Molecular function | Methyltransferase, Transferase |
Biological process | Amino-acid biosynthesis, Methionine biosynthesis |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
UniPathwayi | UPA00051;UER00082 |
Names & Taxonomyi
Protein namesi | Recommended name: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferaseBy similarity (EC:2.1.1.14By similarity)Alternative name(s): Cobalamin-independent methionine synthase1 Publication Pollen allergen Sal k 31 Publication SkMetE1 Publication Vitamin-B12-independent methionine synthaseCurated Allergen: Sal k 3.0101Curated |
Organismi | Kali turgidum (Prickly saltwort) (Salsola kali)Imported |
Taxonomic identifieri | 151250 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › Caryophyllales › Chenopodiaceae › Salsoloideae › Salsoleae › Kali |
Pathology & Biotechi
Allergenic propertiesi
Causes an allergic reaction in human. Binds to IgE in patients allergic to the pollen of prickly saltwort. Both the recombinant 45 kDa N-terminal fragment and the 39 kDa C-terminal fragment of this protein bind to IgE in 67% of the 12 prickly saltwort pollen-allergic patients tested. The same patients have also a positive skin prick test (SPT) response toward these fragments.1 Publication
Keywords - Diseasei
AllergenProtein family/group databases
Allergomei | 8167, Sal k 3 8177, Sal k 3.0101 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000455335 | 1 – ›757 | 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferaseAdd BLAST | ›757 |
Expressioni
Tissue specificityi
Expressed in pollen (at protein level).1 Publication
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 437 – 439 | L-homocysteine bindingBy similarity | 3 | |
Regioni | 437 – 439 | L-methionine bindingBy similarity | 3 | |
Regioni | 521 – 522 | 5-methyltetrahydropteroyltri-L-glutamate bindingBy similarity | 2 |
Sequence similaritiesi
Belongs to the vitamin-B12 independent methionine synthase family.Curated
Family and domain databases
CDDi | cd03311, CIMS_C_terminal_like, 1 hit |
Gene3Di | 3.20.20.210, 2 hits |
HAMAPi | MF_00172, Meth_synth, 1 hit |
InterProi | View protein in InterPro IPR013215, Cbl-indep_Met_Synth_N IPR006276, Cobalamin-indep_Met_synthase IPR002629, Met_Synth_C/arc IPR038071, UROD/MetE-like_sf |
Pfami | View protein in Pfam PF08267, Meth_synt_1, 1 hit PF01717, Meth_synt_2, 1 hit |
PIRSFi | PIRSF000382, MeTrfase_B12_ind, 1 hit |
SUPFAMi | SSF51726, SSF51726, 2 hits |
TIGRFAMsi | TIGR01371, met_syn_B12ind, 1 hit |
i Sequence
Sequence statusi: Fragment.
C1KEU0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASHVVGYPR MGPKRELKFA LESFWDGKSS AEDLKKVAAD LRSSIWKQMA
60 70 80 90 100
DAGIKYIPSN TFAYYDQVLD TTAMLGAVPA RYGFNGGEIG FDLYFSMARG
110 120 130 140 150
NASLPAMEMT KWFDTNYHYI VPELGPEVKF AYSSHKAVDE YKEAKALGVD
160 170 180 190 200
TVPVLVGPVS YLLLSKAAKG VEKSFPLLSL LPKILPVYKE VIAELKAAGA
210 220 230 240 250
STIQFDEPTL VMDLESHQLK AFTDAYADLE STLSGLNVLV ETYFADLTPE
260 270 280 290 300
AYKTLVSLNG VTAFGFDLVR GTKTLDLIKS GFPSGKYLFA GVVDGRNIWA
310 320 330 340 350
NDLAASLATL QSLESIVGKD KLVVSTSCSL LHTAVDLVNE TKLDDEIKSW
360 370 380 390 400
LAFAAQKVLE VNALAKALAG QKDEAFFSAN AAALASRKSS PRVTNEAVQK
410 420 430 440 450
AAAGLKGSDH RRATTVSARL DAQQKKLNLP VLPTTTIGSF PQTVELRRVR
460 470 480 490 500
REYKAKKISE EEYVKAIKEE ISKVVKLQEE LDIDVLVHGE PERNDMVEYF
510 520 530 540 550
GEQLSGFAFT VNGWVQSYGS RCVKPPIIYG DVSRPKAMTV FWSSLAQSMT
560 570 580 590 600
SRPMKGMLTG PVTILNWSFV RNDQPRHETC YQIALAIEDE AEDLEKAGIN
610 620 630 640 650
VIQIDEAALR EGLPLRKSGH GFYLQWAVHS FRITNVGIQD TTQIHTHMCY
660 670 680 690 700
SNFNDIIHSI IDMDADVITI ENSRSDEKLL SVFREGVKYG AGIGPGVYDI
710 720 730 740 750
HSPRIPPTEE LADRIRKMLA VLESNVLWVN PDCGLKTRKY NEVNPALSNM
VYAAKPI
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Non-terminal residuei | 757 | Imported | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | FJ821454 mRNA Translation: ACO34814.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | FJ821454 mRNA Translation: ACO34814.1 |
3D structure databases
SMRi | C1KEU0 |
ModBasei | Search... |
Protein family/group databases
Allergomei | 8167, Sal k 3 8177, Sal k 3.0101 |
Enzyme and pathway databases
UniPathwayi | UPA00051;UER00082 |
Family and domain databases
CDDi | cd03311, CIMS_C_terminal_like, 1 hit |
Gene3Di | 3.20.20.210, 2 hits |
HAMAPi | MF_00172, Meth_synth, 1 hit |
InterProi | View protein in InterPro IPR013215, Cbl-indep_Met_Synth_N IPR006276, Cobalamin-indep_Met_synthase IPR002629, Met_Synth_C/arc IPR038071, UROD/MetE-like_sf |
Pfami | View protein in Pfam PF08267, Meth_synt_1, 1 hit PF01717, Meth_synt_2, 1 hit |
PIRSFi | PIRSF000382, MeTrfase_B12_ind, 1 hit |
SUPFAMi | SSF51726, SSF51726, 2 hits |
TIGRFAMsi | TIGR01371, met_syn_B12ind, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | METE_KALTU | |
Accessioni | C1KEU0Primary (citable) accession number: C1KEU0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 25, 2022 |
Last sequence update: | May 26, 2009 | |
Last modified: | May 25, 2022 | |
This is version 36 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families