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UniProtKB - C1K5M3 (PHS1_SOLLC)
Protein
Beta-phellandrene synthase (neryl-diphosphate-cyclizing), chloroplastic
Gene
PHS1
Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Functioni
Monoterpene synthase catalyzing the production of beta-phellandrene from neryl diphosphate. Produces also lower amounts of delta-2-carene, alpha-phellandrene and limonene. When incubated in vitro with geranyl diphosphate, catalyzes the formation of acyclic myrcene and ocimene as major products in addition to beta-phellandrene.
1 PublicationCatalytic activityi
- EC:4.2.3.511 Publication
Cofactori
Mg2+By similarity
Kineticsi
- KM=9.1 µM for neryl diphosphate1 Publication
- KM=2900 µM for geranyl diphosphate1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 531 | Magnesium 1By similarity | 1 | |
Metal bindingi | 531 | Magnesium 2By similarity | 1 | |
Metal bindingi | 676 | Magnesium 3By similarity | 1 | |
Metal bindingi | 684 | Magnesium 3By similarity | 1 |
GO - Molecular functioni
- magnesium ion binding Source: GO_Central
- terpene synthase activity Source: GO_Central
GO - Biological processi
- diterpenoid biosynthetic process Source: GO_Central
- gibberellin biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Lyase |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BRENDAi | 4.2.3.51, 3101 |
Names & Taxonomyi
Protein namesi | Recommended name: Beta-phellandrene synthase (neryl-diphosphate-cyclizing), chloroplastic (EC:4.2.3.51) |
Gene namesi | Name:PHS1 |
Organismi | Solanum lycopersicum (Tomato) (Lycopersicon esculentum) |
Taxonomic identifieri | 4081 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Solanum subgen. Lycopersicon |
Proteomesi |
|
Subcellular locationi
Chloroplast
- chloroplast Curated
Chloroplast
- chloroplast Source: GO_Central
Keywords - Cellular componenti
Chloroplast, PlastidPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 36 | ChloroplastSequence analysisAdd BLAST | 36 | |
ChainiPRO_0000405121 | 37 – 778 | Beta-phellandrene synthase (neryl-diphosphate-cyclizing), chloroplasticAdd BLAST | 742 |
Proteomic databases
PaxDbi | C1K5M3 |
Expressioni
Tissue specificityi
Trichomes.1 Publication
Gene expression databases
ExpressionAtlasi | C1K5M3, baseline and differential |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 531 – 535 | DDXXD motif | 5 |
Domaini
The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.By similarity
Sequence similaritiesi
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | ENOG502SI1N, Eukaryota |
InParanoidi | C1K5M3 |
OrthoDBi | 247204at2759 |
Family and domain databases
Gene3Di | 1.10.600.10, 1 hit 1.50.10.130, 1 hit |
InterProi | View protein in InterPro IPR008949, Isoprenoid_synthase_dom_sf IPR001906, Terpene_synth_N IPR036965, Terpene_synth_N_sf IPR005630, Terpene_synthase_metal-bd IPR008930, Terpenoid_cyclase/PrenylTrfase |
Pfami | View protein in Pfam PF01397, Terpene_synth, 1 hit PF03936, Terpene_synth_C, 1 hit |
SUPFAMi | SSF48239, SSF48239, 2 hits SSF48576, SSF48576, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
C1K5M3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIVGYRSTII TLSHPKLGNG KTISSNAIFQ RSCRVRCSHS TTSSMNGFED
60 70 80 90 100
ARDRIRESFG KLELSPSSYD TAWVAMVPSR HSLNEPCFPQ CLDWIIENQR
110 120 130 140 150
EDGSWGLNPT HPLLLKDSLS STLACLLALT KWRVGDEQIK RGLGFIETYG
160 170 180 190 200
WAVDNKDQIS PLGFEVIFSS MIKSAEKLDL NLPLNLHLVN LVKCKRDSTI
210 220 230 240 250
KRNVEYMGEG VGELCDWKEM IKLHQRQNGS LFDSPATTAA ALIYHQHDQK
260 270 280 290 300
CYQYLNSIFQ QHKNWVPTMY PTKVHSLLCL VDTLQNLGVH RHFKSEIKKA
310 320 330 340 350
LDEIYRLWQQ KNEQIFSNVT HCAMAFRLLR MSYYDVSSDE LAEFVDEEHF
360 370 380 390 400
FATNGKYKSH VEILELHKAS QLAIDHEKDD ILDKINNWTR AFMEQKLLNN
410 420 430 440 450
GFIDRMSKKE VELALRKFYT TSHLAENRRY IKSYEENNFK ILKAAYRSPN
460 470 480 490 500
INNKDLLAFS IHDFELCQAQ HREELQQLKR WFEDYRLDQL GLAERYIHAS
510 520 530 540 550
YLFGVTVIPE PELSDARLMY AKYVMLLTIV DDHFESFASK DECFNIIELV
560 570 580 590 600
ERWDDYASVG YKSEKVKVFF SVFYKSIEEL ATIAEIKQGR SVKNHLINLW
610 620 630 640 650
LELMKLMLME RVEWCSGKTI PSIEEYLYVT SITFCAKLIP LSTQYFLGIK
660 670 680 690 700
ISKDLLESDE ICGLWNCSGR VMRILNDLQD SKREQKEVSI NLVTLLMKSM
710 720 730 740 750
SEEEAIMKIK EILEMNRREL LKMVLVQKKG SQLPQLCKDI FWRTSKWAHF
760 770
TYSQTDGYRI AEEMKNHIDE VFYKPLNH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | FJ797957 mRNA Translation: ACO56896.1 |
RefSeqi | NP_001234629.1, NM_001247700.1 |
Genome annotation databases
GeneIDi | 100316883 |
KEGGi | sly:100316883 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | FJ797957 mRNA Translation: ACO56896.1 |
RefSeqi | NP_001234629.1, NM_001247700.1 |
3D structure databases
SMRi | C1K5M3 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 4081.Solyc08g005670.2.1 |
Proteomic databases
PaxDbi | C1K5M3 |
Genome annotation databases
GeneIDi | 100316883 |
KEGGi | sly:100316883 |
Phylogenomic databases
eggNOGi | ENOG502SI1N, Eukaryota |
InParanoidi | C1K5M3 |
OrthoDBi | 247204at2759 |
Enzyme and pathway databases
BRENDAi | 4.2.3.51, 3101 |
Gene expression databases
ExpressionAtlasi | C1K5M3, baseline and differential |
Family and domain databases
Gene3Di | 1.10.600.10, 1 hit 1.50.10.130, 1 hit |
InterProi | View protein in InterPro IPR008949, Isoprenoid_synthase_dom_sf IPR001906, Terpene_synth_N IPR036965, Terpene_synth_N_sf IPR005630, Terpene_synthase_metal-bd IPR008930, Terpenoid_cyclase/PrenylTrfase |
Pfami | View protein in Pfam PF01397, Terpene_synth, 1 hit PF03936, Terpene_synth_C, 1 hit |
SUPFAMi | SSF48239, SSF48239, 2 hits SSF48576, SSF48576, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PHS1_SOLLC | |
Accessioni | C1K5M3Primary (citable) accession number: C1K5M3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 8, 2011 |
Last sequence update: | May 26, 2009 | |
Last modified: | February 23, 2022 | |
This is version 54 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families