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Protein

Ketol-acid reductoisomerase (NADP(+))

Gene

ilvC

Organism
Laribacter hongkongensis (strain HLHK9)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.UniRule annotation

Catalytic activityi

(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH.UniRule annotation
(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit.UniRule annotation

Pathwayi: L-isoleucine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-isoleucine from 2-oxobutanoate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase (ilvI)
  2. Ketol-acid reductoisomerase (NADP(+)) (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD)
  4. Branched-chain-amino-acid aminotransferase (ilvE)
This subpathway is part of the pathway L-isoleucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-isoleucine from 2-oxobutanoate, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: L-valine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-valine from pyruvate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase (ilvI)
  2. Ketol-acid reductoisomerase (NADP(+)) (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD)
  4. Branched-chain-amino-acid aminotransferase (ilvE)
This subpathway is part of the pathway L-valine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-valine from pyruvate, the pathway L-valine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei47NADPUniRule annotation1
Binding sitei52NADPUniRule annotation1
Active sitei107UniRule annotation1
Binding sitei133NADP; via amide nitrogenUniRule annotation1
Metal bindingi190Magnesium 1UniRule annotation1
Metal bindingi190Magnesium 2UniRule annotation1
Metal bindingi194Magnesium 1UniRule annotation1
Metal bindingi226Magnesium 2UniRule annotation1
Metal bindingi230Magnesium 2UniRule annotation1
Binding sitei251SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi24 – 27NADPUniRule annotation4
Nucleotide bindingi82 – 85NADPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesis
LigandMagnesium, Metal-binding, NADP

Enzyme and pathway databases

BioCyciLHON557598:G1GCR-148-MONOMER
UniPathwayiUPA00047; UER00056
UPA00049; UER00060

Names & Taxonomyi

Protein namesi
Recommended name:
Ketol-acid reductoisomerase (NADP(+))UniRule annotation (EC:1.1.1.86UniRule annotation)
Short name:
KARIUniRule annotation
Alternative name(s):
Acetohydroxy-acid isomeroreductaseUniRule annotation
Short name:
AHIRUniRule annotation
Alpha-keto-beta-hydroxylacyl reductoisomeraseUniRule annotation
Ketol-acid reductoisomerase type 1UniRule annotation
Ketol-acid reductoisomerase type IUniRule annotation
Gene namesi
Name:ilvCUniRule annotation
Ordered Locus Names:LHK_00158
OrganismiLaribacter hongkongensis (strain HLHK9)
Taxonomic identifieri557598 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesChromobacteriaceaeLaribacter
Proteomesi
  • UP000002010 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001909731 – 338Ketol-acid reductoisomerase (NADP(+))Add BLAST338

Proteomic databases

PRIDEiC1DA41

Interactioni

Protein-protein interaction databases

STRINGi557598.LHK_00158

Structurei

3D structure databases

ProteinModelPortaliC1DA41
SMRiC1DA41
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 181KARI N-terminal RossmannPROSITE-ProRule annotationAdd BLAST181
Domaini182 – 327KARI C-terminal knottedPROSITE-ProRule annotationAdd BLAST146

Sequence similaritiesi

Belongs to the ketol-acid reductoisomerase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C6M Bacteria
COG0059 LUCA
HOGENOMiHOG000016230
KOiK00053
OMAiRAMFSWL
OrthoDBiPOG091H063Y

Family and domain databases

HAMAPiMF_00435 IlvC, 1 hit
InterProiView protein in InterPro
IPR008927 6-PGluconate_DH-like_C_sf
IPR013023 KARI
IPR000506 KARI_C
IPR013116 KARI_N
IPR014359 KARI_prok
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR21371 PTHR21371, 1 hit
PfamiView protein in Pfam
PF01450 IlvC, 1 hit
PF07991 IlvN, 1 hit
PIRSFiPIRSF000116 IlvC_gammaproteo, 1 hit
SUPFAMiSSF48179 SSF48179, 1 hit
SSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00465 ilvC, 1 hit
PROSITEiView protein in PROSITE
PS51851 KARI_C, 1 hit
PS51850 KARI_N, 1 hit

Sequencei

Sequence statusi: Complete.

C1DA41-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVYYDKDAD LSIIRDKKVA IIGYGSQGHA HAQNLKESGV DVIVGLRKDG
60 70 80 90 100
ASWKKAEAAG HTVKEVGEAV KTADVVMILL PDETQPDVYR NDIAPNLKKG
110 120 130 140 150
AALAFAHGFN IHYNQIVPPA DIDVIMIAPK GPGHTVRSEF LKGGGVPSLI
160 170 180 190 200
AVYQDHSGRA RDVALSYAAA NGGTKGGVIE TSFREETETD LFGEQAVLCG
210 220 230 240 250
GAVELVKAGF ETLTEAGYAP EMAYFECLHE LKLIVDLMYE GGIANMNYSI
260 270 280 290 300
SNNAEYGEYV TGPEVITAAS KEAMKKALYR IQSGEYAKMF ILEGKTNYPS
310 320 330
MTARRRLTAD HPIEKVGAEL RAMMPWIAKN KLVDQSKN
Length:338
Mass (Da):36,701
Last modified:May 26, 2009 - v1
Checksum:i1EA5FEDCE2B4AFFC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001154 Genomic DNA Translation: ACO73154.1
RefSeqiWP_012695649.1, NC_012559.1

Genome annotation databases

EnsemblBacteriaiACO73154; ACO73154; LHK_00158
KEGGilhk:LHK_00158

Similar proteinsi

Entry informationi

Entry nameiILVC_LARHH
AccessioniPrimary (citable) accession number: C1DA41
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 26, 2009
Last modified: May 23, 2018
This is version 63 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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