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Protein

S-adenosylmethionine synthase

Gene

metK

Organism
Brevibacillus brevis (strain 47 / JCM 6285 / NBRC 100599)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme.UniRule annotation

Catalytic activityi

ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.UniRule annotation

Cofactori

Protein has several cofactor binding sites:

Pathwayi: S-adenosyl-L-methionine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. S-adenosylmethionine synthase (metK)
This subpathway is part of the pathway S-adenosyl-L-methionine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-adenosyl-L-methionine from L-methionine, the pathway S-adenosyl-L-methionine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei19ATPUniRule annotation1
Metal bindingi21MagnesiumUniRule annotation1
Metal bindingi47PotassiumUniRule annotation1
Binding sitei60MethionineUniRule annotation1
Binding sitei103MethionineUniRule annotation1
Binding sitei254ATP; shared with neighboring subunitUniRule annotation1
Binding sitei254Methionine; shared with neighboring subunitUniRule annotation1
Binding sitei277ATP; via amide nitrogen; shared with neighboring subunitUniRule annotation1
Binding sitei281ATP; shared with neighboring subunitUniRule annotation1
Binding sitei285MethionineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi179 – 181ATPUniRule annotation3
Nucleotide bindingi245 – 246ATPUniRule annotation2
Nucleotide bindingi260 – 261ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferaseUniRule annotationImported
Biological processOne-carbon metabolismUniRule annotation
LigandATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding, PotassiumUniRule annotation

Enzyme and pathway databases

UniPathwayi
UPA00315;UER00080

Names & Taxonomyi

Protein namesi
Recommended name:
S-adenosylmethionine synthaseUniRule annotation (EC:2.5.1.6UniRule annotation)
Short name:
AdoMet synthaseUniRule annotation
Alternative name(s):
MATUniRule annotation
Methionine adenosyltransferaseUniRule annotation
Gene namesi
Name:metKUniRule annotationImported
Ordered Locus Names:BBR47_54050Imported
OrganismiBrevibacillus brevis (strain 47 / JCM 6285 / NBRC 100599)Imported
Taxonomic identifieri358681 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPaenibacillaceaeBrevibacillus
Proteomesi
  • UP000001877 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homotetramer; dimer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi358681.BBR47_54050

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 105S-AdoMet_synt_NInterPro annotationAdd BLAST98
Domaini130 – 246S-AdoMet_synt_MInterPro annotationAdd BLAST117
Domaini248 – 387S-AdoMet_synt_CInterPro annotationAdd BLAST140

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni103 – 113Flexible loopUniRule annotationAdd BLAST11

Sequence similaritiesi

Belongs to the AdoMet synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CPH Bacteria
COG0192 LUCA
HOGENOMiHOG000245710
KOiK00789
OMAiPGHFLFT
OrthoDBiPOG091H00IO

Family and domain databases

HAMAPiMF_00086 S_AdoMet_synth1, 1 hit
InterProiView protein in InterPro
IPR022631 ADOMET_SYNTHASE_CS
IPR022630 S-AdoMet_synt_C
IPR022629 S-AdoMet_synt_central
IPR022628 S-AdoMet_synt_N
IPR002133 S-AdoMet_synthetase
IPR022636 S-AdoMet_synthetase_sfam
PANTHERiPTHR11964 PTHR11964, 1 hit
PfamiView protein in Pfam
PF02773 S-AdoMet_synt_C, 1 hit
PF02772 S-AdoMet_synt_M, 1 hit
PF00438 S-AdoMet_synt_N, 1 hit
PIRSFiPIRSF000497 MAT, 1 hit
SUPFAMiSSF55973 SSF55973, 3 hits
TIGRFAMsiTIGR01034 metK, 1 hit
PROSITEiView protein in PROSITE
PS00376 ADOMET_SYNTHASE_1, 1 hit
PS00377 ADOMET_SYNTHASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

C0Z733-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MALQKGRRLF TSESVTEGHP DKICDQISDS ILDAILSKDP NARVACETSV
60 70 80 90 100
TTGLVLVAGE ITTNTYVDIQ KLVRETIREI GYDRAKFGFD ADTCGVITAI
110 120 130 140 150
GEQSADIALG VDQALEAREG KMTDAEIEAI GAGDQGLMFG FACNETPELM
160 170 180 190 200
PLPISMSHQL ARRLTEVRKN GTLAYLRPDG KTQVTVEYDG DKPVRIDTIV
210 220 230 240 250
ISTQHAPETT LEQIKQDLVE HVIKPVVPAE LLDAETKYFI NPTGRFVIGG
260 270 280 290 300
PQGDAGLTGR KIIVDTYGGY ARHGGGAFSG KDPTKVDRSG AYAARYVAKN
310 320 330 340 350
IVAAGLADKC EVQVAYAIGV AQPVSIAVDT FGTGTISEEA LVKLVRKHFD
360 370 380 390 400
LRPAGIIKQL DLRRPIYKQT AAYGHFGRND LNVPWEQTDK AEVLKQEAAQ

L
Length:401
Mass (Da):43,401
Last modified:May 26, 2009 - v1
Checksum:iA4D2EE7EF199A076
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008955 Genomic DNA Translation: BAH46382.1
RefSeqiWP_015893578.1, NC_012491.1

Genome annotation databases

EnsemblBacteriaiBAH46382; BAH46382; BBR47_54050
GeneIDi29262629
KEGGibbe:BBR47_54050

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008955 Genomic DNA Translation: BAH46382.1
RefSeqiWP_015893578.1, NC_012491.1

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi358681.BBR47_54050

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAH46382; BAH46382; BBR47_54050
GeneIDi29262629
KEGGibbe:BBR47_54050

Phylogenomic databases

eggNOGiENOG4105CPH Bacteria
COG0192 LUCA
HOGENOMiHOG000245710
KOiK00789
OMAiPGHFLFT
OrthoDBiPOG091H00IO

Enzyme and pathway databases

UniPathwayi
UPA00315;UER00080

Family and domain databases

HAMAPiMF_00086 S_AdoMet_synth1, 1 hit
InterProiView protein in InterPro
IPR022631 ADOMET_SYNTHASE_CS
IPR022630 S-AdoMet_synt_C
IPR022629 S-AdoMet_synt_central
IPR022628 S-AdoMet_synt_N
IPR002133 S-AdoMet_synthetase
IPR022636 S-AdoMet_synthetase_sfam
PANTHERiPTHR11964 PTHR11964, 1 hit
PfamiView protein in Pfam
PF02773 S-AdoMet_synt_C, 1 hit
PF02772 S-AdoMet_synt_M, 1 hit
PF00438 S-AdoMet_synt_N, 1 hit
PIRSFiPIRSF000497 MAT, 1 hit
SUPFAMiSSF55973 SSF55973, 3 hits
TIGRFAMsiTIGR01034 metK, 1 hit
PROSITEiView protein in PROSITE
PS00376 ADOMET_SYNTHASE_1, 1 hit
PS00377 ADOMET_SYNTHASE_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiC0Z733_BREBN
AccessioniPrimary (citable) accession number: C0Z733
Entry historyiIntegrated into UniProtKB/TrEMBL: May 26, 2009
Last sequence update: May 26, 2009
Last modified: March 28, 2018
This is version 62 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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