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Protein

Curcumin synthase 1

Gene

CURS1

Organism
Curcuma longa (Turmeric) (Curcuma domestica)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of curcumin by condensing feruloyl-CoA with a diketide-CoA in the curcuminoid biosynthesis.2 Publications

Catalytic activityi

Feruloyl-CoA + feruloylacetyl-CoA + H2O = 2 CoA + curcumin + CO2.2 Publications

Kineticsi

Kcat is 1.1 min(-1) with feruloyl-CoA. Kcat is 0.85 min(-1) with p-coumaroyl-CoA.
  1. KM=18 µM for feruloyl-CoA1 Publication
  2. KM=189 µM for p-coumaroyl-CoA1 Publication

    pH dependencei

    Optimum pH is 9.0.1 Publication

    Temperature dependencei

    Optimum temperature is 50 degrees Celsius.1 Publication

    Pathwayi: flavonoid biosynthesis

    This protein is involved in the pathway flavonoid biosynthesis, which is part of Secondary metabolite biosynthesis.1 Publication
    View all proteins of this organism that are known to be involved in the pathway flavonoid biosynthesis and in Secondary metabolite biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei164By similarity1

    GO - Molecular functioni

    • curcumin synthase activity Source: UniProtKB-EC
    • protein homodimerization activity Source: UniProtKB
    • transferase activity, transferring acyl groups other than amino-acyl groups Source: UniProtKB

    GO - Biological processi

    Keywordsi

    Molecular functionAcyltransferase, Transferase
    Biological processFlavonoid biosynthesis

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-15409
    BRENDAi2.3.1.217 9125
    2.3.1.219 9125
    UniPathwayi
    UPA00154

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Curcumin synthase 1 (EC:2.3.1.217)
    Gene namesi
    Name:CURS1
    OrganismiCurcuma longa (Turmeric) (Curcuma domestica)
    Taxonomic identifieri136217 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaZingiberalesZingiberaceaeCurcuma

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi211G → F or W: Strong reduction in enzyme activity. 1 Publication1
    Mutagenesisi303H → A or Q: Strong reduction in enzyme activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004225711 – 389Curcumin synthase 1Add BLAST389

    Expressioni

    Tissue specificityi

    Expressed in both the leaf and rhizome, with higher expression in the rhizome.1 Publication

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    GO - Molecular functioni

    Structurei

    Secondary structure

    1389
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details

    3D structure databases

    ProteinModelPortaliC0SVZ6
    SMRiC0SVZ6
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the chalcone/stilbene synthases family.Curated

    Phylogenomic databases

    KOiK13234

    Family and domain databases

    Gene3Di3.40.47.10, 2 hits
    InterProiView protein in InterPro
    IPR012328 Chalcone/stilbene_synth_C
    IPR001099 Chalcone/stilbene_synthase_N
    IPR011141 Polyketide_synthase_type-III
    IPR016039 Thiolase-like
    PANTHERiPTHR11877 PTHR11877, 1 hit
    PfamiView protein in Pfam
    PF02797 Chal_sti_synt_C, 1 hit
    PF00195 Chal_sti_synt_N, 1 hit
    PIRSFiPIRSF000451 PKS_III, 1 hit
    SUPFAMiSSF53901 SSF53901, 2 hits

    Sequencei

    Sequence statusi: Complete.

    C0SVZ6-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MANLHALRRE QRAQGPATIM AIGTATPPNL YEQSTFPDFY FRVTNSDDKQ
    60 70 80 90 100
    ELKKKFRRMC EKTMVKKRYL HLTEEILKER PKLCSYKEAS FDDRQDIVVE
    110 120 130 140 150
    EIPRLAKEAA EKAIKEWGRP KSEITHLVFC SISGIDMPGA DYRLATLLGL
    160 170 180 190 200
    PLTVNRLMIY SQACHMGAAM LRIAKDLAEN NRGARVLVVA CEITVLSFRG
    210 220 230 240 250
    PNEGDFEALA GQAGFGDGAG AVVVGADPLE GIEKPIYEIA AAMQETVAES
    260 270 280 290 300
    QGAVGGHLRA FGWTFYFLNQ LPAIIADNLG RSLERALAPL GVREWNDVFW
    310 320 330 340 350
    VAHPGNWAII DAIEAKLQLS PDKLSTARHV FTEYGNMQSA TVYFVMDELR
    360 370 380
    KRSAVEGRST TGDGLQWGVL LGFGPGLSIE TVVLRSMPL
    Length:389
    Mass (Da):43,034
    Last modified:May 26, 2009 - v1
    Checksum:iD6532FE43817B2F3
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB495007 mRNA Translation: BAH56226.1

    Genome annotation databases

    KEGGiag:BAH56226

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB495007 mRNA Translation: BAH56226.1

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3OV2X-ray2.32A/B/C/D1-389[»]
    3OV3X-ray2.50A/B/C/D1-389[»]
    ProteinModelPortaliC0SVZ6
    SMRiC0SVZ6
    ModBaseiSearch...
    MobiDBiSearch...

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    KEGGiag:BAH56226

    Phylogenomic databases

    KOiK13234

    Enzyme and pathway databases

    UniPathwayi
    UPA00154

    BioCyciMetaCyc:MONOMER-15409
    BRENDAi2.3.1.217 9125
    2.3.1.219 9125

    Family and domain databases

    Gene3Di3.40.47.10, 2 hits
    InterProiView protein in InterPro
    IPR012328 Chalcone/stilbene_synth_C
    IPR001099 Chalcone/stilbene_synthase_N
    IPR011141 Polyketide_synthase_type-III
    IPR016039 Thiolase-like
    PANTHERiPTHR11877 PTHR11877, 1 hit
    PfamiView protein in Pfam
    PF02797 Chal_sti_synt_C, 1 hit
    PF00195 Chal_sti_synt_N, 1 hit
    PIRSFiPIRSF000451 PKS_III, 1 hit
    SUPFAMiSSF53901 SSF53901, 2 hits
    ProtoNetiSearch...

    Entry informationi

    Entry nameiCURS1_CURLO
    AccessioniPrimary (citable) accession number: C0SVZ6
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2013
    Last sequence update: May 26, 2009
    Last modified: November 22, 2017
    This is version 36 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health

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