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UniProtKB - C0SJS4 (C71AJ_APIGR)
Protein
Psoralen synthase
Gene
CYP71AJ2
Organism
Apium graveolens (Celery)
Status
Functioni
Involved in linear furanocumarin (psoralen) biosynthesis. Converts marmesin to psoralen and, with much lower affinity, 5-hydroxymarmesin to bergaptol.
1 PublicationCatalytic activityi
- (7S)-marmesin + O2 + reduced [NADPH—hemoprotein reductase] = acetone + H+ + 2 H2O + oxidized [NADPH—hemoprotein reductase] + psoralen1 PublicationEC:1.14.14.1411 Publication
Kineticsi
- KM=0.54 µM for marmesin1 Publication
- KM=54 µM for 5-hydroxymarmesin1 Publication
pH dependencei
Optimum pH is 7.5.1 Publication
GO - Molecular functioni
- heme binding Source: InterPro
- iron ion binding Source: InterPro
- psoralen synthase activity Source: UniProtKB-EC
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Ligand | Heme, Iron, Metal-binding |
Enzyme and pathway databases
BRENDAi | 1.14.14.141, 388 |
Names & Taxonomyi
Protein namesi | Recommended name: Psoralen synthase (EC:1.14.14.1411 Publication)Alternative name(s): Cytochrome P450 CYP71AJ2 |
Gene namesi | Name:CYP71AJ2 |
Organismi | Apium graveolens (Celery) |
Taxonomic identifieri | 4045 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › asterids › campanulids › Apiales › Apiaceae › Apioideae › apioid superclade › Apieae › Apium |
Subcellular locationi
Endoplasmic reticulum
- Microsome membrane By similarity; Single-pass membrane protein By similarity
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB-KW
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 1 – 18 | HelicalBy similarityAdd BLAST | 18 |
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, MicrosomePathology & Biotechi
Biotechnological usei
Psoralen possesses photocarcinogen properties. It intercalates in double-stranded DNA and cross-links pyrimidine bases under UV-A irradiation. Psoralen is widely used in combination with UV-A radiation to treat a variety of skin disorders like psoriasis or eczema.
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000401482 | ‹1 – ›476 | Psoralen synthaseAdd BLAST | ›476 |
Family & Domainsi
Sequence similaritiesi
Belongs to the cytochrome P450 family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixFamily and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
i Sequence
Sequence statusi: Fragment.
C0SJS4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
YFFSLFLVTV FVYKLLTLKK TPSKNLPPSP PRYPIIGNLH QIGPDPQHSL
60 70 80 90 100
RDLALKYGPL MSLKFGTVPV LVVSSADAAR EVLKTHDLIF ADRPYSSVAN
110 120 130 140 150
KVFYNGKDMV FARYTEYWRQ VKSICVTQLL SNKRVNSFQN VREEEVDLLV
160 170 180 190 200
QNIENSCSKV INLTELLIEV TGNVVCKVSV GSGDKVDSYK ILILEIMEML
210 220 230 240 250
GYSRSIEDFF PMFGWVDWLT GLRGKVAKAA KGVDDFLEGV LKEHLTARAS
260 270 280 290 300
NNASADNDFV SILLEIQEAD AGSTMDNECI KSLIWDMLGA GTETISTALE
310 320 330 340 350
WTLAALIKNP DAMLKLQNEV REIGKGKSKI SEADLGKMTY LQAVMKESMR
360 370 380 390 400
LYFTAPLLVP RESRQDVKFM GYDISAGTQV LINVWAIARD PSLWEKPEEF
410 420 430 440 450
RPERFLNSHI DYKGFNYEYL PFGAGRRGCP GIQFAMAVNE LVVANVIHKF
460 470
NFELPDGERL EDLDMTAVSG ITLRKK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Non-terminal residuei | 1 | 1 | ||
Non-terminal residuei | 476 | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EF191022 mRNA Translation: ABO84855.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EF191022 mRNA Translation: ABO84855.1 |
3D structure databases
SMRi | C0SJS4 |
ModBasei | Search... |
Enzyme and pathway databases
BRENDAi | 1.14.14.141, 388 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | C71AJ_APIGR | |
Accessioni | C0SJS4Primary (citable) accession number: C0SJS4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 30, 2010 |
Last sequence update: | May 5, 2009 | |
Last modified: | June 2, 2021 | |
This is version 40 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |