UniProtKB - C0SJS3 (ANGS_PASSA)
Angelicin synthase
CYP71AJ4
Functioni
Involved in the biosynthesis of coumarins and furanocoumarins (FCs), natural products required for defense responses against attacks by predators with potential medical and agroindustrial usages such as anticoagulant, rodenticide and artificial vanilla substitutes (PubMed:29971079).
Involved in angular furanocumarin biosynthesis. Converts (+)-columbianetin to angelicin (PubMed:19098286).
Produces also some hydroxycolumbianetin as a by-product (PubMed:19098286).
No activity with demethylsuberosin, (+)-marmesin, 5-hydroxymarmesin, psoralen, bergaptol, xanthotoxol, bergapten, xanthotoxin, isopimpinellin, cinnamic acid, 4-coumaric acid, 2-coumaric acid, ferulic acid, coumarin, herniarin, scopoletin, or umbelliferone (PubMed:19098286).
The reaction mechanism is initiated by the abstraction of syn-C-3'-hydrogen (PubMed:19098286).
1 Publication1 PublicationCatalytic activityi
- (S)-columbianetin + O2 + reduced [NADPH—hemoprotein reductase] = acetone + angelicin + H+ + 2 H2O + oxidized [NADPH—hemoprotein reductase]1 PublicationEC:1.14.14.1481 Publication
Cofactori
Activity regulationi
Kineticsi
- KM=2.1 µM for (+)-columbianetin1 Publication
- KM=1.4 µM for syn-[3'-D]columbianetin1 Publication
pH dependencei
Temperature dependencei
: Secondary metabolite biosynthesis Pathwayi
This protein is involved in Secondary metabolite biosynthesis.1 PublicationView all proteins of this organism that are known to be involved in Secondary metabolite biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 109 | Substrate specificityBy similarity | 1 | |
Sitei | 293 | Substrate specificityBy similarity | 1 | |
Sitei | 297 | Substrate specificityBy similarity | 1 | |
Metal bindingi | 431 | Iron (heme axial ligand)By similarity | 1 |
GO - Molecular functioni
- angelicin synthase activity Source: UniProtKB-EC
- heme binding Source: InterPro
- iron ion binding Source: InterPro
GO - Biological processi
- coumarin biosynthetic process Source: UniProtKB
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Ligand | Heme, Iron, Metal-binding |
Enzyme and pathway databases
BRENDAi | 1.14.14.148, 4562 |
Names & Taxonomyi
Protein namesi | Recommended name: Angelicin synthase1 Publication (EC:1.14.14.1481 Publication)Alternative name(s): Cytochrome P450 CYP71AJ41 Publication |
Gene namesi | Name:CYP71AJ41 Publication |
Organismi | Pastinaca sativa (Wild parsnip) (Anethum pastinaca) |
Taxonomic identifieri | 4041 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › asterids › campanulids › Apiales › Apiaceae › Apioideae › apioid superclade › Tordylieae › Tordyliinae › Pastinaca |
Subcellular locationi
Endoplasmic reticulum
- Microsome membrane By similarity; Single-pass membrane protein Sequence analysis
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB-KW
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 1 – 17 | HelicalSequence analysisAdd BLAST | 17 |
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, MicrosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000401480 | ‹1 – ›478 | Angelicin synthaseAdd BLAST | ›478 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 357 – 361 | Substrate specificityBy similarity | 5 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixFamily and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
i Sequence
Sequence statusi: Fragment.
10 20 30 40 50
YFFSLFLVTI FLYKWLAKKT PSKNLPPSPP RLPIIGNLHQ IGPDLHISLR
60 70 80 90 100
DLARKYGPLM QLQLGRIPVL VVSSAEATRE VLKTHDVVFS QRPITSAIDK
110 120 130 140 150
LCYKGRDVAF SRYSEYWRQV RSTCVTQLLS NSRVHSFHNI REEEVALLIQ
160 170 180 190 200
NIENSASEVI NLGEQLIQLT RNVVCRVSVG SEYLSGHKGK LYQKLLAEVT
210 220 230 240 250
EMLAYTYSIG DFIPLLGWVD WLSGSKAKVE KTAKEVDAFL EGALRDHIKT
260 270 280 290 300
MASNKGSAND DFLSILLEIR EADAGSTLDE ECIKAIVWDM ILGGTETTST
310 320 330 340 350
TLEWIVAAII KNPDVMFKLQ KEVREIGKGK SKIEEVDLVK MNYLKAVMKE
360 370 380 390 400
SMRLYITAFL LPREAKQDVK LMGYDISSGT QVLINTWETA RDPSLWDNPE
410 420 430 440 450
EFRPERFLNS PIDYKGLHYE YLPFGGGRRG CPGIQFAMAV NELAVANVVY
460 470
KFDFKMPDGE RFEDLDMSGV PGISLYRK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Non-terminal residuei | 1 | 1 | ||
Non-terminal residuei | 478 | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EF191021 mRNA Translation: ABO84854.1 |
Genome annotation databases
KEGGi | ag:ABO84854 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | EF191021 mRNA Translation: ABO84854.1 |
3D structure databases
SMRi | C0SJS3 |
ModBasei | Search... |
Genome annotation databases
KEGGi | ag:ABO84854 |
Enzyme and pathway databases
BRENDAi | 1.14.14.148, 4562 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ANGS_PASSA | |
Accessioni | C0SJS3Primary (citable) accession number: C0SJS3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 30, 2010 |
Last sequence update: | May 5, 2009 | |
Last modified: | February 23, 2022 | |
This is version 45 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families