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Protein

Lipoyl synthase 2, chloroplastic

Gene

LIP1P-2

Organism
Zea mays (Maize)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Miscellaneous

This protein may be expected to contain an N-terminal transit peptide but none has been predicted.UniRule annotation

Catalytic activityi

Protein N6-(octanoyl)lysine + an [Fe-S] cluster scaffold protein carrying a [4Fe-4S]2+ cluster + 2 S-adenosyl-L-methionine + 2 oxidized [2Fe-2S] ferredoxin + 6 H+ = protein N6-(dihydrolipoyl)lysine + an [Fe-S] cluster scaffold protein + 2 sulfide + 4 Fe3+ + 2 L-methionine + 2 5'-deoxyadenosine + 2 reduced [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Lipoyl synthase 2, chloroplastic (LIP1P-2), Lipoyl synthase, mitochondrial (LIP1), Lipoyl synthase 1, chloroplastic (LIP1P-1), Lipoyl synthase, chloroplastic (LIP1P), Lipoyl synthase, mitochondrial (LIP1), Lipoyl synthase, mitochondrial (100384288), Lipoyl synthase, chloroplastic (LIP1P), Lipoyl synthase, chloroplastic (LIP1P), Lipoyl synthase, chloroplastic (100384288), Lipoyl synthase, chloroplastic (LIP1P)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi84Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi89Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi95Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi121Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi125Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi128Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionTransferase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayi
UPA00538;UER00593

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase 2, chloroplastic (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthase 2UniRule annotation
Short name:
LS 2UniRule annotation
Short name:
Lip-syn 2UniRule annotation
Lipoate synthase, plastidial 2UniRule annotation
Short name:
LIP1p 2UniRule annotation
Lipoic acid synthase 2UniRule annotation
Gene namesi
Name:LIP1P-2UniRule annotation
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogonodaeAndropogoneaeTripsacinaeZea
Proteomesi
  • UP000007305 Componenti: Chromosome 3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003988611 – 367Lipoyl synthase 2, chloroplasticAdd BLAST367

Proteomic databases

PaxDbiC0PN26
PRIDEiC0PN26

Expressioni

Gene expression databases

ExpressionAtlasiC0PN26 differential
GenevisibleiC0PN26 ZM

Interactioni

Protein-protein interaction databases

STRINGi4577.GRMZM2G101698_P02

Structurei

3D structure databases

ProteinModelPortaliC0PN26
SMRiC0PN26
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2672 Eukaryota
COG0320 LUCA
HOGENOMiHOG000235998
KOiK03644
OMAiDSSENAM
OrthoDBiEOG093604CP

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00206 Lipoyl_synth, 1 hit
MF_03129 Lipoyl_synth_plantC, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR003698 Lipoyl_synth
IPR027526 Lipoyl_synth_chlpt
IPR007197 rSAM
PANTHERiPTHR10949:SF14 PTHR10949:SF14, 1 hit
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF005963 Lipoyl_synth, 1 hit
SFLDiSFLDF00271 lipoyl_synthase, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00510 lipA, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 7 potential isoforms that are computationally mapped.iShow all

C0PN26-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MQSSLARPPV LAGCGERRGL AAVARCRAEA AAPVWTASRA SAGPYTGRDP
60 70 80 90 100
EVKKPAWLRQ RAAQGEKYAR LRESIGELKL NTVCVEAQCP NIGECWNGGG
110 120 130 140 150
GAGGEGDGIA TATIMVLGDT CTRGCRFCAV KTSNKPPPPD PLEPLNTALA
160 170 180 190 200
VASWGVDYVV LTSVDRDDLP DGGSSHFAQT VKALKELKPG ILVECLTSDF
210 220 230 240 250
RGDLEAISSL ANSGLDVYAH NIETVRSLQR VVRDPRAGYD QSLAVLKHAK
260 270 280 290 300
GSREDMITKS SIMLGLGETD EEVKQAMMDL RAIGVDILTL GQYLQPSERH
310 320 330 340 350
LTVREYVTPQ KFQFWKEYGE SVGFRYVASG PLVRSSYRAG ELFIQNLVRN
360
NKTESSMDPY AEITKSN
Length:367
Mass (Da):39,767
Last modified:May 5, 2009 - v1
Checksum:i1D6C0E80C60C5A41
GO

Computationally mapped potential isoform sequencesi

There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1D6NAK4A0A1D6NAK4_MAIZE
Lipoyl synthase, chloroplastic
100384288 LIP1P, ZEAMMB73_Zm00001d043328
356Annotation score:
A0A1R3PWZ0A0A1R3PWZ0_MAIZE
Lipoyl synthase, chloroplastic
LIP1P ZEAMMB73_Zm00001d043328
367Annotation score:
K7V4H8K7V4H8_MAIZE
Lipoyl synthase, chloroplastic
LIP1P ZEAMMB73_Zm00001d012656
361Annotation score:
A0A1D6NAK2A0A1D6NAK2_MAIZE
Lipoyl synthase, mitochondrial
100384288 ZEAMMB73_Zm00001d043328
330Annotation score:
A0A1D6GAM2A0A1D6GAM2_MAIZE
Lipoyl synthase, chloroplastic
LIP1P ZEAMMB73_Zm00001d012656
341Annotation score:
A0A1D6GAM0A0A1D6GAM0_MAIZE
Lipoyl synthase, chloroplastic
LIP1P ZEAMMB73_Zm00001d012656
384Annotation score:
A0A1D6GAL9A0A1D6GAL9_MAIZE
Lipoyl synthase 1, chloroplastic
ZEAMMB73_Zm00001d012656
181Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT069695 mRNA Translation: ACN36592.1
RefSeqiNP_001170321.1, NM_001176850.1
UniGeneiZm.123157
Zm.155719

Genome annotation databases

EnsemblPlantsiZm00001d043328_T003; Zm00001d043328_P003; Zm00001d043328
GeneIDi100384288
GrameneiZm00001d043328_T003; Zm00001d043328_P003; Zm00001d043328
KEGGizma:100384288

Similar proteinsi

Entry informationi

Entry nameiLISC2_MAIZE
AccessioniPrimary (citable) accession number: C0PN26
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: May 5, 2009
Last modified: July 18, 2018
This is version 65 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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