UniProtKB - C0LGR3 (RGI3_ARATH)
LRR receptor-like serine/threonine-protein kinase RGI3
RGI3
Functioni
Together with RGI1, RGI2, RGI4 and RGI5, acts as receptor of RGF peptides (e.g. RGF1, GLV5/CLEL1/RGF2, GLV7/CLEL3/RGF3, GLV3/RGF4, GLV10/CLEL7/RGF5 and RGF10/CLELN), peptide hormones which maintain the postembryonic root stem cell niche by regulating the expression levels and patterns of the transcription factor PLETHORA (PLT, e.g. PLT1 and PLT2) (PubMed:27229311, PubMed:27229312, PubMed:27001831).
Links RGF peptides signal with their downstream components (PubMed:27229311, PubMed:27001831).
3 PublicationsCatalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 788 | ATPPROSITE-ProRule annotation | 1 | |
Sitei | 788 | Essential for autophosphorylation activityBy similarity | 1 | |
Active sitei | 883 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 766 – 774 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- peptide binding Source: UniProtKB
- peptide receptor activity Source: UniProtKB
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: UniProtKB-KW
- protein serine kinase activity Source: RHEA
GO - Biological processi
- maintenance of meristem identity Source: UniProtKB
- maintenance of root meristem identity Source: UniProtKB
- regulation of root development Source: UniProtKB
- regulation of root meristem growth Source: TAIR
Keywordsi
Molecular function | Kinase, Receptor, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: LRR receptor-like serine/threonine-protein kinase RGI3Curated (EC:2.7.11.1PROSITE-ProRule annotation)Alternative name(s): Protein RECEPTOR OF RGF1 11 Publication Protein RGF1 INSENSITIVE 31 Publication |
Gene namesi | Name:RGI31 Publication Synonyms:RGFR11 Publication, RGFR31 Publication Ordered Locus Names:At4g26540Imported ORF Names:M3E9.30Imported |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT4G26540 |
TAIRi | locus:2131518, AT4G26540 |
Subcellular locationi
Plasma membrane
- Cell membrane Sequence analysis; Single-pass type I membrane protein Sequence analysis
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 25 – 703 | ExtracellularSequence analysisAdd BLAST | 679 | |
Transmembranei | 704 – 724 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 725 – 1091 | CytoplasmicSequence analysisAdd BLAST | 367 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 24 | Sequence analysisAdd BLAST | 24 | |
ChainiPRO_0000387553 | 25 – 1091 | LRR receptor-like serine/threonine-protein kinase RGI3Add BLAST | 1067 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 40 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Disulfide bondi | 57 ↔ 64 | By similarity | ||
Glycosylationi | 104 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Glycosylationi | 163 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Glycosylationi | 356 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Glycosylationi | 431 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Glycosylationi | 452 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Glycosylationi | 604 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Glycosylationi | 651 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Glycosylationi | 697 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Modified residuei | 831 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 870 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 933 | PhosphotyrosineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Glycoprotein, Phosphoprotein, Ubl conjugationProteomic databases
PaxDbi | C0LGR3 |
PRIDEi | C0LGR3 |
ProteomicsDBi | 236934 |
PTM databases
iPTMneti | C0LGR3 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
ExpressionAtlasi | C0LGR3, baseline and differential |
Genevisiblei | C0LGR3, AT |
Interactioni
Subunit structurei
Binds to RGF peptides such as RGF1, GLV5/CLEL1/RGF2, GLV7/CLEL3/RGF3, GLV3/RGF4, GLV10/CLEL7/RGF5 and RGF10/CLELN; these interactions trigger the formation of heterodimers with SERK1, SERK2 or BAK1/SERK3 via LRR regions.
2 PublicationsBinary interactionsi
C0LGR3
With | #Exp. | IntAct |
---|---|---|
At1g69990 [C0LGI5] | 3 | EBI-20659695,EBI-20651225 |
Protein-protein interaction databases
BioGRIDi | 14047, 44 interactors |
IntActi | C0LGR3, 44 interactors |
STRINGi | 3702.AT4G26540.1 |
Structurei
Secondary structure
3D structure databases
SMRi | C0LGR3 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 33 – 56 | LRR 1Sequence analysisAdd BLAST | 24 | |
Repeati | 67 – 91 | LRR 2Sequence analysisAdd BLAST | 25 | |
Repeati | 92 – 115 | LRR 3Sequence analysisAdd BLAST | 24 | |
Repeati | 116 – 140 | LRR 4Sequence analysisAdd BLAST | 25 | |
Repeati | 142 – 166 | LRR 5Sequence analysisAdd BLAST | 25 | |
Repeati | 168 – 188 | LRR 6Sequence analysisAdd BLAST | 21 | |
Repeati | 190 – 213 | LRR 7Sequence analysisAdd BLAST | 24 | |
Repeati | 214 – 237 | LRR 8Sequence analysisAdd BLAST | 24 | |
Repeati | 239 – 261 | LRR 9Sequence analysisAdd BLAST | 23 | |
Repeati | 262 – 285 | LRR 10Sequence analysisAdd BLAST | 24 | |
Repeati | 287 – 309 | LRR 11Sequence analysisAdd BLAST | 23 | |
Repeati | 311 – 332 | LRR 12Sequence analysisAdd BLAST | 22 | |
Repeati | 333 – 357 | LRR 13Sequence analysisAdd BLAST | 25 | |
Repeati | 359 – 383 | LRR 14Sequence analysisAdd BLAST | 25 | |
Repeati | 385 – 405 | LRR 15Sequence analysisAdd BLAST | 21 | |
Repeati | 406 – 429 | LRR 16Sequence analysisAdd BLAST | 24 | |
Repeati | 431 – 453 | LRR 17Sequence analysisAdd BLAST | 23 | |
Repeati | 454 – 477 | LRR 18Sequence analysisAdd BLAST | 24 | |
Repeati | 478 – 501 | LRR 19Sequence analysisAdd BLAST | 24 | |
Repeati | 503 – 524 | LRR 20Sequence analysisAdd BLAST | 22 | |
Repeati | 525 – 548 | LRR 21Sequence analysisAdd BLAST | 24 | |
Repeati | 549 – 572 | LRR 22Sequence analysisAdd BLAST | 24 | |
Repeati | 574 – 596 | LRR 23Sequence analysisAdd BLAST | 23 | |
Repeati | 598 – 620 | LRR 24Sequence analysisAdd BLAST | 23 | |
Repeati | 621 – 644 | LRR 25Sequence analysisAdd BLAST | 24 | |
Repeati | 645 – 668 | LRR 26Sequence analysisAdd BLAST | 24 | |
Repeati | 669 – 690 | LRR 27Sequence analysisAdd BLAST | 22 | |
Domaini | 760 – 1046 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 287 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 173 – 174 | Small peptide recognitionCombined sources1 Publication | 2 | |
Motifi | 195 – 198 | Small peptide recognitionCombined sources1 Publication | 4 | |
Motifi | 218 – 223 | Small peptide recognitionCombined sources1 Publication | 6 | |
Motifi | 246 | Small peptide recognitionCombined sources1 Publication | 1 | |
Motifi | 268 – 270 | Small peptide recognitionCombined sources1 Publication | 3 | |
Motifi | 316 – 319 | Small peptide recognitionCombined sources1 Publication | 4 | |
Motifi | 338 – 340 | Small peptide recognitionCombined sources1 Publication | 3 | |
Motifi | 386 – 390 | Small peptide recognitionCombined sources1 Publication | 5 | |
Motifi | 412 – 415 | Small peptide recognitionCombined sources1 Publication | 4 | |
Motifi | 434 – 438 | Small peptide recognitionCombined sources1 Publication | 5 | |
Motifi | 458 – 460 | Small peptide recognitionCombined sources1 Publication | 3 |
Sequence similaritiesi
Keywords - Domaini
Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QSU9, Eukaryota |
HOGENOMi | CLU_000288_22_1_1 |
InParanoidi | C0LGR3 |
OMAi | PNTPFFH |
OrthoDBi | 826997at2759 |
PhylomeDBi | C0LGR3 |
Family and domain databases
Gene3Di | 3.80.10.10, 7 hits |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR001611, Leu-rich_rpt IPR003591, Leu-rich_rpt_typical-subtyp IPR032675, LRR_dom_sf IPR013210, LRR_N_plant-typ IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF13855, LRR_8, 3 hits PF08263, LRRNT_2, 1 hit PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00369, LRR_TYP, 8 hits SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MPPNIYRLSF FSSLLCFFFI PCFSLDQQGQ ALLSWKSQLN ISGDAFSSWH
60 70 80 90 100
VADTSPCNWV GVKCNRRGEV SEIQLKGMDL QGSLPVTSLR SLKSLTSLTL
110 120 130 140 150
SSLNLTGVIP KEIGDFTELE LLDLSDNSLS GDIPVEIFRL KKLKTLSLNT
160 170 180 190 200
NNLEGHIPME IGNLSGLVEL MLFDNKLSGE IPRSIGELKN LQVLRAGGNK
210 220 230 240 250
NLRGELPWEI GNCENLVMLG LAETSLSGKL PASIGNLKRV QTIAIYTSLL
260 270 280 290 300
SGPIPDEIGY CTELQNLYLY QNSISGSIPT TIGGLKKLQS LLLWQNNLVG
310 320 330 340 350
KIPTELGNCP ELWLIDFSEN LLTGTIPRSF GKLENLQELQ LSVNQISGTI
360 370 380 390 400
PEELTNCTKL THLEIDNNLI TGEIPSLMSN LRSLTMFFAW QNKLTGNIPQ
410 420 430 440 450
SLSQCRELQA IDLSYNSLSG SIPKEIFGLR NLTKLLLLSN DLSGFIPPDI
460 470 480 490 500
GNCTNLYRLR LNGNRLAGSI PSEIGNLKNL NFVDISENRL VGSIPPAISG
510 520 530 540 550
CESLEFLDLH TNSLSGSLLG TTLPKSLKFI DFSDNALSST LPPGIGLLTE
560 570 580 590 600
LTKLNLAKNR LSGEIPREIS TCRSLQLLNL GENDFSGEIP DELGQIPSLA
610 620 630 640 650
ISLNLSCNRF VGEIPSRFSD LKNLGVLDVS HNQLTGNLNV LTDLQNLVSL
660 670 680 690 700
NISYNDFSGD LPNTPFFRRL PLSDLASNRG LYISNAISTR PDPTTRNSSV
710 720 730 740 750
VRLTILILVV VTAVLVLMAV YTLVRARAAG KQLLGEEIDS WEVTLYQKLD
760 770 780 790 800
FSIDDIVKNL TSANVIGTGS SGVVYRITIP SGESLAVKKM WSKEESGAFN
810 820 830 840 850
SEIKTLGSIR HRNIVRLLGW CSNRNLKLLF YDYLPNGSLS SRLHGAGKGG
860 870 880 890 900
CVDWEARYDV VLGVAHALAY LHHDCLPTII HGDVKAMNVL LGPHFEPYLA
910 920 930 940 950
DFGLARTISG YPNTGIDLAK PTNRPPMAGS YGYMAPEHAS MQRITEKSDV
960 970 980 990 1000
YSYGVVLLEV LTGKHPLDPD LPGGAHLVKW VRDHLAEKKD PSRLLDPRLD
1010 1020 1030 1040 1050
GRTDSIMHEM LQTLAVAFLC VSNKANERPL MKDVVAMLTE IRHIDVGRSE
1060 1070 1080 1090
TEKIKAGGCG SKEPQQFMSN EKIINSHGSS NCSFAFSDDS V
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 221 | L → P in AAL32011 (PubMed:14593172).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL022223 Genomic DNA Translation: CAA18216.1 Sequence problems. AL161565 Genomic DNA Translation: CAB79509.1 Sequence problems. CP002687 Genomic DNA Translation: AEE85216.1 AF436829 mRNA Translation: AAL32011.1 Frameshift. FJ708754 mRNA Translation: ACN59348.1 |
PIRi | T05050 |
RefSeqi | NP_567748.5, NM_118787.6 |
Genome annotation databases
EnsemblPlantsi | AT4G26540.1; AT4G26540.1; AT4G26540 |
GeneIDi | 828760 |
Gramenei | AT4G26540.1; AT4G26540.1; AT4G26540 |
KEGGi | ath:AT4G26540 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL022223 Genomic DNA Translation: CAA18216.1 Sequence problems. AL161565 Genomic DNA Translation: CAB79509.1 Sequence problems. CP002687 Genomic DNA Translation: AEE85216.1 AF436829 mRNA Translation: AAL32011.1 Frameshift. FJ708754 mRNA Translation: ACN59348.1 |
PIRi | T05050 |
RefSeqi | NP_567748.5, NM_118787.6 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5HYX | X-ray | 2.60 | B | 57-689 | [»] | |
5HZ0 | X-ray | 2.56 | B | 57-689 | [»] | |
5HZ1 | X-ray | 2.59 | B | 57-689 | [»] | |
5HZ3 | X-ray | 2.86 | B | 57-689 | [»] | |
SMRi | C0LGR3 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 14047, 44 interactors |
IntActi | C0LGR3, 44 interactors |
STRINGi | 3702.AT4G26540.1 |
PTM databases
iPTMneti | C0LGR3 |
Proteomic databases
PaxDbi | C0LGR3 |
PRIDEi | C0LGR3 |
ProteomicsDBi | 236934 |
Genome annotation databases
EnsemblPlantsi | AT4G26540.1; AT4G26540.1; AT4G26540 |
GeneIDi | 828760 |
Gramenei | AT4G26540.1; AT4G26540.1; AT4G26540 |
KEGGi | ath:AT4G26540 |
Organism-specific databases
Araporti | AT4G26540 |
TAIRi | locus:2131518, AT4G26540 |
Phylogenomic databases
eggNOGi | ENOG502QSU9, Eukaryota |
HOGENOMi | CLU_000288_22_1_1 |
InParanoidi | C0LGR3 |
OMAi | PNTPFFH |
OrthoDBi | 826997at2759 |
PhylomeDBi | C0LGR3 |
Miscellaneous databases
PROi | PR:C0LGR3 |
Gene expression databases
ExpressionAtlasi | C0LGR3, baseline and differential |
Genevisiblei | C0LGR3, AT |
Family and domain databases
Gene3Di | 3.80.10.10, 7 hits |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR001611, Leu-rich_rpt IPR003591, Leu-rich_rpt_typical-subtyp IPR032675, LRR_dom_sf IPR013210, LRR_N_plant-typ IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF13855, LRR_8, 3 hits PF08263, LRRNT_2, 1 hit PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00369, LRR_TYP, 8 hits SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RGI3_ARATH | |
Accessioni | C0LGR3Primary (citable) accession number: C0LGR3 Secondary accession number(s): O65580, Q8W556 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 3, 2009 |
Last sequence update: | May 5, 2009 | |
Last modified: | February 23, 2022 | |
This is version 90 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families