UniProtKB - C0HL66 (H33A_DROME)
Protein
Histone H3.3A
Gene
His3.3A
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes and is specifically enriched in modifications associated with active chromatin. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.1 Publication
Miscellaneous
This histone is the predominant form in non-dividing cells.
GO - Molecular functioni
- DNA binding Source: UniProtKB
- protein heterodimerization activity Source: InterPro
- structural constituent of chromatin Source: FlyBase
GO - Biological processi
- DNA replication-independent nucleosome assembly Source: FlyBase
- male meiotic nuclear division Source: FlyBase
- nucleosome assembly Source: UniProtKB
Keywordsi
Molecular function | DNA-binding |
Enzyme and pathway databases
Reactomei | R-DME-212300, PRC2 methylates histones and DNA R-DME-2559580, Oxidative Stress Induced Senescence R-DME-2559582, Senescence-Associated Secretory Phenotype (SASP) R-DME-427359, SIRT1 negatively regulates rRNA expression R-DME-5578749, Transcriptional regulation by small RNAs R-DME-5625886, Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 R-DME-73772, RNA Polymerase I Promoter Escape R-DME-8936459, RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function R-DME-9018519, Estrogen-dependent gene expression R-DME-983231, Factors involved in megakaryocyte development and platelet production |
Names & Taxonomyi
Protein namesi | Recommended name: Histone H3.3A1 PublicationAlternative name(s): H3.AImported |
Gene namesi | ORF Names:CG5825Imported |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0014857, His3.3A |
Subcellular locationi
Nucleus
- Nucleus By similarity
Other locations
- Chromosome By similarity
Nucleus
- nucleus Source: GO_Central
Other locations
- nucleosome Source: FlyBase
- polytene chromosome Source: FlyBase
Keywords - Cellular componenti
Chromosome, Nucleosome core, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000221302 | 2 – 136 | Histone H3.3AAdd BLAST | 135 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 5 | N6,N6,N6-trimethyllysine; alternate1 Publication | 1 | |
Modified residuei | 5 | N6,N6-dimethyllysine; alternate1 Publication | 1 | |
Modified residuei | 5 | N6-methyllysine; alternate1 Publication | 1 | |
Modified residuei | 10 | N6,N6-dimethyllysine; alternate1 Publication | 1 | |
Modified residuei | 10 | N6-acetyllysine; alternate2 Publications | 1 | |
Modified residuei | 10 | N6-methyllysine; alternate1 Publication | 1 | |
Modified residuei | 11 | PhosphoserineBy similarity | 1 | |
Modified residuei | 15 | N6,N6-dimethyllysine; alternate1 Publication | 1 | |
Modified residuei | 15 | N6-acetyllysine; alternate3 Publications | 1 | |
Modified residuei | 15 | N6-methyllysine; alternate1 Publication | 1 | |
Modified residuei | 19 | N6-acetyllysine1 Publication | 1 | |
Modified residuei | 24 | N6-acetyllysine1 Publication | 1 | |
Modified residuei | 28 | N6,N6,N6-trimethyllysine; alternate1 Publication | 1 | |
Modified residuei | 28 | N6,N6-dimethyllysine; alternate1 Publication | 1 | |
Modified residuei | 28 | N6-methyllysine; alternate1 Publication | 1 | |
Modified residuei | 37 | N6,N6-dimethyllysine; alternate1 Publication | 1 | |
Modified residuei | 37 | N6-methyllysine; alternate1 Publication | 1 | |
Modified residuei | 38 | N6,N6-dimethyllysine; alternate1 Publication | 1 | |
Modified residuei | 38 | N6-methyllysine; alternate1 Publication | 1 | |
Modified residuei | 57 | N6-succinyllysine1 Publication | 1 | |
Modified residuei | 80 | N6,N6-dimethyllysine; alternate2 Publications | 1 | |
Modified residuei | 80 | N6-methyllysine; alternate2 Publications | 1 | |
Modified residuei | 80 | N6-succinyllysine; alternate1 Publication | 1 |
Post-translational modificationi
Phosphorylation at Ser-11 by aurB/ial during mitosis and meiosis is crucial for chromosome condensation and cell-cycle progression. Phosphorylation at Ser-11 by JIL-1 during interphase is linked to gene activation and restricts the formation of heterochromatin at inappropriate sites. Phosphorylation at Ser-11 is enriched on male X chromosome compared to the autosome.4 Publications
Acetylation is generally linked to gene activation. Acetylated on Lys-15 during prophase I of meiosis. Phosphorylation of H2A 'Thr-119' is a prerequisite for H3 Lys-15 acetylation. Acetylation on Lys-15 is enriched on male X chromosome compared to the autosome.3 Publications
Methylation at Lys-5 or Lys-80 is generally associated with active chromatin. Methylation at Lys-80 by gpp occurs at low levels in specific developmental stages and tissues undergoing active cell division, and at highest levels in epidermal cells undergoing differentiation.2 Publications
Keywords - PTMi
Acetylation, Methylation, PhosphoproteinPTM databases
iPTMneti | C0HL66 |
Expressioni
Tissue specificityi
High levels of expression in ovaries and relatively weak expression in the testes. Highest levels of expression in embryos.1 Publication
Gene expression databases
ExpressionAtlasi | C0HL66, baseline and differential |
Genevisiblei | P84249, DM |
Interactioni
Subunit structurei
The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
Interacts with Daxx (via C-terminus) (PubMed:28320872).
1 PublicationGO - Molecular functioni
- protein heterodimerization activity Source: InterPro
Family & Domainsi
Sequence similaritiesi
Belongs to the histone H3 family.Curated
Phylogenomic databases
OMAi | CTMARTK |
OrthoDBi | 1564596at2759 |
Family and domain databases
Gene3Di | 1.10.20.10, 1 hit |
InterProi | View protein in InterPro IPR009072, Histone-fold IPR007125, Histone_H2A/H2B/H3 IPR000164, Histone_H3/CENP-A |
PANTHERi | PTHR11426, PTHR11426, 1 hit |
Pfami | View protein in Pfam PF00125, Histone, 1 hit |
PRINTSi | PR00622, HISTONEH3 |
SMARTi | View protein in SMART SM00428, H3, 1 hit |
SUPFAMi | SSF47113, SSF47113, 1 hit |
PROSITEi | View protein in PROSITE PS00322, HISTONE_H3_1, 1 hit PS00959, HISTONE_H3_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
C0HL66-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MARTKQTARK STGGKAPRKQ LATKAARKSA PSTGGVKKPH RYRPGTVALR
60 70 80 90 100
EIRRYQKSTE LLIRKLPFQR LVREIAQDFK TDLRFQSAAI GALQEASEAY
110 120 130
LVGLFEDTNL CAIHAKRVTI MPKDIQLARR IRGERA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X82257 mRNA Translation: CAA57712.1 AE014134 Genomic DNA Translation: AAF52213.1 AE014134 Genomic DNA Translation: AAN10526.2 AE014134 Genomic DNA Translation: AGB92618.1 AY071057 mRNA Translation: AAL48679.1 AY119629 mRNA Translation: AAM50283.1 KX531337 mRNA Translation: ANY27147.1 |
PIRi | S61220 |
RefSeqi | NP_001259374.1, NM_001272445.2 NP_001260082.1, NM_001273153.1 NP_511095.1, NM_078540.5 NP_523479.1, NM_078755.3 NP_723056.2, NM_164624.3 NP_727314.1, NM_167180.4 NP_788892.1, NM_176719.1 |
Genome annotation databases
EnsemblMetazoai | FBtr0071345; FBpp0071280; FBgn0004828 FBtr0071346; FBpp0071281; FBgn0004828 FBtr0071347; FBpp0071282; FBgn0004828 FBtr0079010; FBpp0078649; FBgn0014857 FBtr0333536; FBpp0305716; FBgn0014857 FBtr0333537; FBpp0305717; FBgn0014857 FBtr0340343; FBpp0309302; FBgn0004828 |
GeneIDi | 31848 33736 |
KEGGi | dme:Dmel_CG5825 dme:Dmel_CG8989 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X82257 mRNA Translation: CAA57712.1 AE014134 Genomic DNA Translation: AAF52213.1 AE014134 Genomic DNA Translation: AAN10526.2 AE014134 Genomic DNA Translation: AGB92618.1 AY071057 mRNA Translation: AAL48679.1 AY119629 mRNA Translation: AAM50283.1 KX531337 mRNA Translation: ANY27147.1 |
PIRi | S61220 |
RefSeqi | NP_001259374.1, NM_001272445.2 NP_001260082.1, NM_001273153.1 NP_511095.1, NM_078540.5 NP_523479.1, NM_078755.3 NP_723056.2, NM_164624.3 NP_727314.1, NM_167180.4 NP_788892.1, NM_176719.1 |
3D structure databases
SMRi | C0HL66 |
ModBasei | Search... |
PTM databases
iPTMneti | C0HL66 |
Genome annotation databases
EnsemblMetazoai | FBtr0071345; FBpp0071280; FBgn0004828 FBtr0071346; FBpp0071281; FBgn0004828 FBtr0071347; FBpp0071282; FBgn0004828 FBtr0079010; FBpp0078649; FBgn0014857 FBtr0333536; FBpp0305716; FBgn0014857 FBtr0333537; FBpp0305717; FBgn0014857 FBtr0340343; FBpp0309302; FBgn0004828 |
GeneIDi | 31848 33736 |
KEGGi | dme:Dmel_CG5825 dme:Dmel_CG8989 |
Organism-specific databases
CTDi | 31848 33736 |
FlyBasei | FBgn0014857, His3.3A |
Phylogenomic databases
OMAi | CTMARTK |
OrthoDBi | 1564596at2759 |
Enzyme and pathway databases
Reactomei | R-DME-212300, PRC2 methylates histones and DNA R-DME-2559580, Oxidative Stress Induced Senescence R-DME-2559582, Senescence-Associated Secretory Phenotype (SASP) R-DME-427359, SIRT1 negatively regulates rRNA expression R-DME-5578749, Transcriptional regulation by small RNAs R-DME-5625886, Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3 R-DME-73772, RNA Polymerase I Promoter Escape R-DME-8936459, RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function R-DME-9018519, Estrogen-dependent gene expression R-DME-983231, Factors involved in megakaryocyte development and platelet production |
Miscellaneous databases
PROi | PR:C0HL66 |
Gene expression databases
ExpressionAtlasi | C0HL66, baseline and differential |
Genevisiblei | P84249, DM |
Family and domain databases
Gene3Di | 1.10.20.10, 1 hit |
InterProi | View protein in InterPro IPR009072, Histone-fold IPR007125, Histone_H2A/H2B/H3 IPR000164, Histone_H3/CENP-A |
PANTHERi | PTHR11426, PTHR11426, 1 hit |
Pfami | View protein in Pfam PF00125, Histone, 1 hit |
PRINTSi | PR00622, HISTONEH3 |
SMARTi | View protein in SMART SM00428, H3, 1 hit |
SUPFAMi | SSF47113, SSF47113, 1 hit |
PROSITEi | View protein in PROSITE PS00322, HISTONE_H3_1, 1 hit PS00959, HISTONE_H3_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | H33A_DROME | |
Accessioni | C0HL66Primary (citable) accession number: C0HL66 Secondary accession number(s): A4V466 Q9V3W4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 28, 2018 |
Last sequence update: | February 28, 2018 | |
Last modified: | December 2, 2020 | |
This is version 18 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families