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Protein

Basic phospholipase A2 daboxin P

Gene
N/A
Organism
Daboia russelii (Russel's viper) (Vipera russelii)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Snake venom phospholipase A2 (PLA2) that exhibits anticoagulant activity, probably by binding to factor X and its activated form factor Xa (F10). Shows no cytotoxicity. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.1 Publication

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.PROSITE-ProRule annotation1 Publication

Cofactori

Ca2+1 Publication

Kineticsi

  1. KM=6.6 mM for diheptanoyl thio-phosphatidylcholine1 Publication
  1. Vmax=1.14 mmol/min/mg enzyme1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi27Calcium; via carbonyl oxygenBy similarity1
Metal bindingi29Calcium; via carbonyl oxygenBy similarity1
Metal bindingi31Calcium; via carbonyl oxygenBy similarity1
Active sitei47PROSITE-ProRule annotation1
Metal bindingi48CalciumBy similarity1
Active sitei89PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionBlood coagulation cascade inhibiting toxin, Hemostasis impairing toxin, Hydrolase, Toxin
Biological processLipid degradation, Lipid metabolism
LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Basic phospholipase A2 daboxin P1 Publication (EC:3.1.1.4PROSITE-ProRule annotation1 Publication)
Short name:
svPLA2Curated
Alternative name(s):
Phosphatidylcholine 2-acylhydrolaseCurated
OrganismiDaboia russelii (Russel's viper) (Vipera russelii)
Taxonomic identifieri8707 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeViperinaeDaboia

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004368481 – 121Basic phospholipase A2 daboxin PAdd BLAST121

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi26 ↔ 115By similarity
Disulfide bondi28 ↔ 44By similarity
Disulfide bondi43 ↔ 95By similarity
Disulfide bondi49 ↔ 121By similarity
Disulfide bondi50 ↔ 88By similarity
Disulfide bondi57 ↔ 81By similarity
Disulfide bondi75 ↔ 86By similarity

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.1 Publication

Structurei

3D structure databases

SMRiC0HK16
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

CDDicd00125 PLA2c, 1 hit
Gene3Di1.20.90.10, 1 hit
InterProiView protein in InterPro
IPR001211 PLipase_A2
IPR033112 PLipase_A2_Asp_AS
IPR016090 PLipase_A2_dom
IPR036444 PLipase_A2_dom_sf
IPR033113 PLipase_A2_His_AS
PANTHERiPTHR11716 PTHR11716, 1 hit
PfamiView protein in Pfam
PF00068 Phospholip_A2_1, 1 hit
PRINTSiPR00389 PHPHLIPASEA2
SMARTiView protein in SMART
SM00085 PA2c, 1 hit
SUPFAMiSSF48619 SSF48619, 1 hit
PROSITEiView protein in PROSITE
PS00119 PA2_ASP, 1 hit
PS00118 PA2_HIS, 1 hit

Sequencei

Sequence statusi: Complete.

C0HK16-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
SLLEFGKMIL EETGKLAIPS YSSYGCYCGW GGKGTPKDAT DRCCFVHDCC
60 70 80 90 100
YGNLPDCNNK SKRYRYKKVN GAIVCEKGTS CENRICECDK AAAICFRQNL
110 120
NTYSKKYMLY PDFLCKGELV C
Length:121
Mass (Da):13,612
Last modified:July 6, 2016 - v1
Checksum:iFCDD41EBB1C9EF29
GO

Mass spectrometryi

Molecular mass is 13597.62±1.28 Da from positions 1 - 121. Determined by ESI. 1 Publication

Similar proteinsi

Cross-referencesi

3D structure databases

SMRiC0HK16
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

CDDicd00125 PLA2c, 1 hit
Gene3Di1.20.90.10, 1 hit
InterProiView protein in InterPro
IPR001211 PLipase_A2
IPR033112 PLipase_A2_Asp_AS
IPR016090 PLipase_A2_dom
IPR036444 PLipase_A2_dom_sf
IPR033113 PLipase_A2_His_AS
PANTHERiPTHR11716 PTHR11716, 1 hit
PfamiView protein in Pfam
PF00068 Phospholip_A2_1, 1 hit
PRINTSiPR00389 PHPHLIPASEA2
SMARTiView protein in SMART
SM00085 PA2c, 1 hit
SUPFAMiSSF48619 SSF48619, 1 hit
PROSITEiView protein in PROSITE
PS00119 PA2_ASP, 1 hit
PS00118 PA2_HIS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPA2BD_DABRR
AccessioniPrimary (citable) accession number: C0HK16
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 6, 2016
Last sequence update: July 6, 2016
Last modified: July 18, 2018
This is version 10 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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